We narrowed to 890 results for: ANT;
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TypeBlog PostPublishedMarch 30, 2023, 1:15 p.m....Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...
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25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....calling this “physical distancing” since it’s important to stay virtually connected with the people in...webinars. Share your work online. Maybe you’ve always wanted to start using social media professionally but ... -
Addgene Welcomes John Doench to the Advisory Board
TypeBlog PostPublishedAug. 13, 2015, 2:30 p.m....his love for science and later realizing that he wanted to continue doing research, Doench went on to apply...regulatory process. This early research is still relevant to his work at the Broad Institute today, where... -
Plasmids 101: How to Verify Your Plasmid Using a Restriction Digest Analysis
TypeBlog PostPublishedAug. 28, 2014, 3:34 p.m....circular DNA takes on several conformations the most abundant being: supercoiled, relaxed and nicked. If your...your uncut lane then there is something wrong! Quantify your DNA. Loading too much DNA will make it difficult... -
New and Upcoming Viral Vectors - May 2020
TypeBlog PostPublishedMay 28, 2020, 1:15 p.m....tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter... -
Pooled CRISPR Libraries Offer Genome-Wide Control for Large-Scale Functional Screens
TypeBlog PostPublishedFeb. 24, 2015, 7:50 p.m.... genome-wide screening,” Konermann said. “If you want to activate all 20,000 genes, it is not possible...genes that, when turned on, make melanoma cells resistant to treatment with a BRAF inhibitor. (The test ... -
Lighting Up Cell Signaling with Photoswitchable Kinases
TypeBlog PostPublishedJuly 25, 2017, 1:20 p.m....all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ... -
CRISPRainbow and Genome Visualization
TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and... -
Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages
TypeBlog PostPublishedJan. 11, 2017, 3:30 p.m....preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe... up on this issue. These salary raises are an important step forward in helping the postdoctoral population... -
Celebrating 15 Years of Scientific Sharing
TypeBlog PostPublishedJan. 14, 2019, 2:08 p.m....journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship... -
Summer SciComm Series: Cognitive Load
TypeBlog PostPublishedJune 16, 2022, 1:15 p.m....for your audience. I ask myself that question constantly (and so should you!) Amount of information and... too little)? The answer is: it depends! The important point here is to pay attention to exactly what... -
Developing Lab Management Software for Biology
TypeBlog PostPublishedAug. 25, 2016, 2:30 p.m....of said containers, think carefully about how you want your software to treat those encoded numbers. Loosely...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true... -
Popular Retroviral Vectors and Their Uses in Scientific Research
TypeBlog PostPublishedJuly 24, 2018, 12:51 p.m....lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic... -
Getting Started with Behavioral Neuroscience: Standardizing Experiments and Using Viral Vectors
TypeBlog PostPublishedAug. 20, 2019, 12:31 p.m....Modifying gene expression Generally speaking, one may want to achieve either inhibition or activation of neurons...Sena-Esteves M. Gene transfer to the CNS using recombinant adeno-associated virus. Curr Protoc Microbiol... -
Fluorescent Tagging of Endogenous Genes with SapTrap
TypeBlog PostPublishedOct. 12, 2017, 2:26 p.m.... perform high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it...scarless tag insertion. In addition to the obvious advantages SapTrap offers for tagging a single genetic locus... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....plasmid page (discussed below). Great! Now, if I want to use this plasmid in a publication, how do I know...the plasmid is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are... -
Rett Syndrome: A History of Research and Therapeutic Outlooks
TypeBlog PostPublishedNov. 19, 2020, 2:15 p.m....CRISPR/Cas9 and RNA-mediated correction of MECP2 mutant alleles, in particular, have shown exciting and... (AAV) vector. The editase repaired half of the mutant MECP2 RNA in each of three neuronal subtypes of... -
Twenty Years of Sharing: Expanding Accessibility
TypeBlog PostPublishedNov. 21, 2024, 10:47 p.m....and the distances the packages needed to travel meant the accessibility varied country to country. Figure...to identify and reduce barriers for scientists wanting to share their tools with Addgene. In our next... -
How to Deposit Your Plasmids with Addgene
TypeBlog PostPublishedFeb. 27, 2024, 2:15 p.m.... grown using standard conditions (37 °C, LB, antibiotics, etc.) and stored as glycerol stocks We will...and alternative name boxes as needed. Use the "Relevant mutations/deletions" box to describe any mutations... -
Plasmids 101 eBook 4th Edition
TypeBlog PostPublishedSept. 5, 2023, 1:15 p.m....don't have to take our word for it! “When people want to learn about plasmids, they don’t go to Wikipedia...