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We narrowed to 895 results for: ANT;

Showing: 781 - 800 of 895 results

  1. Multiplexed Overexpression of Regulatory Factors (MORF) collection

    Type
    Blog Post
    Published
    March 30, 2023, 1:15 p.m.
    ...Zhang lab’s previously published protocol and its relevant modifications on the pooled library webpage. Disease... that any researcher could use to generate the relevant disease model. Accurate modeling of a disease ...

  2. 25 Work From Home Tips for Scientists

    Type
    Blog Post
    Published
    March 19, 2020, 1:15 p.m.
    ...calling this “physical distancing” since it’s important to stay virtually connected with the people in...webinars. Share your work online. Maybe you’ve always wanted to start using social media professionally but ...

  3. Visualizing Genomic Loci with CRISPR-Sirius

    Type
    Blog Post
    Published
    April 18, 2019, 12:53 p.m.
    ... different fluorescent protein to various Cas9 variants, SpCas9, NmCas9, and St1Cas9. These fusions localized...low-copy genomic loci or single copy genes. If you want to visualize genomic loci brightly, give CRISPR-...

  4. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...bacteria are permeable to different classes of antibiotics. The classification system is also useful in ...inserted into a specific bacterium, it can be important to consider the type of bacteria it is being used...

  5. Pushing the Limits of DNA Assembly

    Type
    Blog Post
    Published
    Sept. 6, 2017, 1:01 p.m.
    ...while still creating scarless plasmid inserts.  Want to Learn More about DNA Assembly? Check Out Our ...polymerase used in the amplification. This tool allows instant updates as fragments are added, deleted, or reordered...

  6. Addgene Welcomes John Doench to the Advisory Board

    Type
    Blog Post
    Published
    Aug. 13, 2015, 2:30 p.m.
    ...his love for science and later realizing that he wanted to continue doing research, Doench went on to apply...regulatory process. This early research is still relevant to his work at the Broad Institute today, where...

  8. New and Upcoming Viral Vectors - May 2020

    Type
    Blog Post
    Published
    May 28, 2020, 1:15 p.m.
    ...tools for light mediated neuronal control. The advantages of optogenetics lie in the spatiotemporal control...recombination of lox sites. This system takes advantage of the extensive supply of transgenic reporter...

  9. OCaMP: A New Calcium Indicator for Neural Imaging

    Type
    Blog Post
    Published
    Aug. 19, 2025, 1:15 p.m.
    ...using existing indicators. For example, you might want to use OCaMP to monitor Ca2+ signals, while using... pAAV CMV The Lohman lab has a limited quantity of AAVs that can be requested by contacting Alexander...

  11. Celebrating 15 Years of Scientific Sharing

    Type
    Blog Post
    Published
    Jan. 14, 2019, 2:08 p.m.
    ...journals like Nature and in mainstream media like The Atlantic. We’ve been in documentaries in 3 languages (Japanese...with flying colors. While of course always being vigilant about resources, I’m not worried about the stewardship...

  12. Summer SciComm Series: Cognitive Load

    Type
    Blog Post
    Published
    June 16, 2022, 1:15 p.m.
    ...for your audience. I ask myself that question constantly (and so should you!)  Amount of information and... too little)?  The answer is: it depends! The important point here is to pay attention to exactly what...

  13. Lighting Up Cell Signaling with Photoswitchable Kinases

    Type
    Blog Post
    Published
    July 25, 2017, 1:20 p.m.
    ...all-optical psMEK1 + ERK KTR-mRuby2 system has several advantages over existing methods. First, it avoids activating...protein of interest. If you plan to try this, it’s important to check that the function of the protein and ...

  14. CRISPRainbow and Genome Visualization

    Type
    Blog Post
    Published
    Feb. 28, 2017, 3:30 p.m.
    ...similar to that of a comparable FISH method. Importantly, they did not observe labeling when a. no gRNA... other orthologs. Ma et al. tested their dCas9 variants using gRNAs specific for telomeric sequences and...

  15. Uncertainty about Labor Law Brings More Uncertainty to Postdoc Wages

    Type
    Blog Post
    Published
    Jan. 11, 2017, 3:30 p.m.
    ...preliminary injunction against the FLSA updates was granted nationwide. Again, we at Future of Research believe... up on this issue. These salary raises are an important step forward in helping the postdoctoral population...

  16. Developing Lab Management Software for Biology

    Type
    Blog Post
    Published
    Aug. 25, 2016, 2:30 p.m.
    ...of said containers, think carefully about how you want your software to treat those encoded numbers. Loosely...being treated in the lab, because of this, it is important to write loosely coupled, modular code. Use tried-and-true...

  17. Popular Retroviral Vectors and Their Uses in Scientific Research

    Type
    Blog Post
    Published
    July 24, 2018, 12:51 p.m.
    ...lentiCRISPR v1. Special note from the Zhang lab: We are constantly improving our CRISPR reagents. Please check www.genome-engineering.org...ecotropic or amphotropic MuLE lentivirus provides significant genetic power and allows high throughput genetic...

  19. Fluorescent Tagging of Endogenous Genes with SapTrap

    Type
    Blog Post
    Published
    Oct. 12, 2017, 2:26 p.m.
    ... perform high-throughput experiments. If a user wants to carry out a high-throughput genetic screen, it...scarless tag insertion.   In addition to the obvious advantages SapTrap offers for tagging a single genetic locus...

  20. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...plasmid page (discussed below). Great! Now, if I want to use this plasmid in a publication, how do I know...the plasmid is shipped. Copy number (if known), antibiotic resistance, and optimal growth temperature are...
Showing: 781 - 800 of 895 results