We narrowed to 807 results for: App;
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TypeBlog PostPublishedAug. 6, 2015, 2:30 p.m....institutions are not local but are national issues. I applied and was successfully granted a travel award to ...
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Experimenting in an Open Source lab: from CRISPR to Cats
TypeBlog PostPublishedJuly 23, 2015, 2:30 p.m.... in biology but don’t really know how to start applying their skills to it. The Kittybiome project gives... -
AAV Vector Quality Control: Going the Extra Mile with NGS
TypeBlog PostPublishedSept. 12, 2017, 1:44 p.m....suspected, we proceed to de novo assembly of the unmapped reads, employing our bioinformatics software to... -
Quick Guide to Working with Drosophila Part 1: Getting Started with Flies
TypeBlog PostPublishedJuly 13, 2017, 2:30 p.m....but have run into a wall trying to study it? It happens. Is it an evolutionarily conserved gene? Can you... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....expertise available in your lab or organization, and approvals from the organization overseeing your work. Good... -
Transferable Skills Guide: Managing a Team
TypeBlog PostPublishedJan. 30, 2018, 2:42 p.m.... couple of weeks now”. I had no idea this was happening even though I met with X on a weekly basis! The... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....those flames about? Flame logo: The flame that appears beside some of Addgene’s plasmids is an indication... -
Genome-wide Screening Using CRISPR
TypeBlog PostPublishedAug. 18, 2015, 2:30 p.m....negatives – As with any experiment, the use of appropriate controls, multiple replicates and several cell... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....substantial. It's estimated that genetic engineering as applied to products on the domestic market generates $300... -
CRISPR 101: RNA Editing with Cas13
TypeBlog PostPublishedJuly 31, 2020, 12:30 p.m....Addressing the potential use of RESCUE in therapeutic applications, the authors tested various dRanCas13b truncations... -
Negative Can Be Positive: Open AAV Data with Addgene
TypeBlog PostPublishedSept. 6, 2022, 1:15 p.m....this information can be cited, tracked, and appropriately credited, broadening the knowledge base of AAV... -
Deep Dive: Fixing and Permeabilizing for Immunofluorescence
TypeBlog PostPublishedAug. 30, 2022, 1:15 p.m....the same drawbacks to each reagent type, so this approach is not suitable for all antibodies. Detergents... -
DNA Purification Without a Kit
TypeBlog PostPublishedFeb. 11, 2020, 2:15 p.m....rather a glass powder slurry that has a milk-like appearance. To make DNA stick to the glass particles in ... -
Simplify Cloning with in vivo Assembly
TypeBlog PostPublishedOct. 18, 2018, 12:37 p.m....protocol prior to transformation (Figure 1). As this approach requires no special reagents or purification kits... -
Plasmids 101: Protein tags
TypeBlog PostPublishedDec. 11, 2014, 4:26 p.m....deciding on which one to use will depend on your application (see table below). The antibodies available for... -
Save Time with Transient Plant Leaf Transformations
TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m....placed in an Agrobacterium solution and a vacuum is applied with a vacuum bell or desiccator. Successful infiltration... -
Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names
TypeBlog PostPublishedApril 2, 2019, 1:44 p.m....Often times, a gene gets its name based on what happens to an organism when a gene/protein is depleted ... -
CRISPR-mediated Plant Base Editors
TypeBlog PostPublishedJan. 3, 2019, 1:35 p.m....question probably comes to mind: can CRISPR be applied to fix the defective base precisely? Until 2016... -
The PAM Requirement and Expanding CRISPR Beyond SpCas9
TypeBlog PostPublishedAug. 20, 2020, 2:30 p.m....to dramatically increase the number of CRISPR applications. The first non-Cas9 CRISPR protein adapted for... -
Plasmids 101: Stringent Regulation of Replication
TypeBlog PostPublishedJune 20, 2024, 1:15 p.m....carefully regulated to ensure that it occurs at the appropriate time—for example, the cell must have enough nutrients...