We narrowed to 878 results for: IND
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TypeBlog PostPublishedApril 26, 2022, 1:15 p.m.... Sybr Green is a fluorescent dye that works by binding to all double-stranded DNA, while Taqman uses an...variance in your variant options, you’re likely to find an application that matches your needs. Happy quantifying...
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Multiplexed Capture of Promoter-enhancer 3D Chromatin Structures Using CRISPR
TypeBlog PostPublishedJuly 2, 2020, 12:40 p.m....allows CAPTURE 2.0 to be used with primary cells. Find plasmids for CAPTURE 2.0! When compared to the original... CREs Here Additional Resources on Addgene.org Find Other Cas9 Variants for Purifying Genomic Regions... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....wavelengths (See this handy emission spectra chart to find your antibody's spectra). Your cytometer will therefore...diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value... -
Light Sheet Fluorescence Microscopy
TypeBlog PostPublishedJuly 16, 2015, 2:30 p.m....the University of Miami Miller School of Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas...the University of Miami Miller School of Medicine. Find plasmids from the Tsoulfas lab here. References... -
Transgenic Organisms, Cas9 Gene Drives, and Appropriate Safeguards
TypeBlog PostPublishedMay 22, 2015, 4:58 p.m.... to build a gene drive in a model organism would find our work and consequently use these precautions ...geographic area where escaped organisms won't be able to find mates2. For example, ongoing experiments attempting... -
Plasmids 101: Multicistronic Vectors
TypeBlog PostPublishedSept. 9, 2014, 8:20 p.m....-expression by using a plasmid with multiple, individual expression cassettes, having the genes expressed... the ability to spark translation initiation independent of the 5’ cap. IRES elements are very useful... -
Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection
TypeBlog PostPublishedDec. 12, 2013, 3:35 p.m....science with BioBricks The BioBrick Foundation's Linda Kahl explains the Addgene collaboration this way...analyzing human language and observed that we humans find it useful to describe and interact with the world... -
Viral Vectors 101: The Retroviral Lifecycle
TypeBlog PostPublishedJuly 27, 2023, 1:15 p.m....2015). Total Virus and Bacteria Concentrations in Indoor and Outdoor Air. Environmental Science & Technology...RT and IN incorporated into HIV-1 particles independently of the Gag/Pol precursor protein. The EMBO Journal... -
Plasmids 101: Colony PCR
TypeBlog PostPublishedMay 12, 2016, 2:30 p.m.... insert-specific sequence. This is a “yes or no” kind of test, with a positive clone amplifying a product...insert-specific and back-bone specific primers. Find Empty Backbones at Addgene Each approach has advantages... -
The Importance of a Fun Workplace: Company Culture at Addgene
TypeBlog PostPublishedSept. 3, 2015, 2:30 p.m....collaborators have completed their work thoroughly. It’s kind of a chicken and egg situation- do we work so hard...rose-colored-glasses-esque picture of how everyone is kind and collaborative and all we do is have fun all ... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....information or clarification on these vectors. Read on to find the answers to our most frequently asked lentiviral... designed specifically for transient expression (find some useful backbones here) or from viral transduction... -
Advancing Biology with Zebrafish: Genetic Tools for Developmental Studies and More
TypeBlog PostPublishedAug. 13, 2019, 12:58 p.m....which are less prolific and more expensive per individual to maintain. Also in contrast to mice, the transparent... collection Read our molecular biology reference Find plasmids for your research ... -
New Coomassie Protocol Video
TypeBlog PostPublishedOct. 31, 2023, 1:15 p.m.... proteins. If this video is helpful, you can find our complete collection of protocol videos on the... -
New Western Blot Protocol Video!
TypeBlog PostPublishedMarch 28, 2023, 1:15 p.m....out all of our protocols on our Protocol page, and find our protocol videos on our YouTube page. ... -
Thanks to Addgene Depositors, We’re Now Sharing over 100,000 Reagents!
TypeBlog PostPublishedApril 8, 2021, 7:25 p.m....depositing labs (currently at 4,651 as of April 8, 2021) Find these stats and more on the addgene.org homepage... -
Introducing...Addgene Quick Tips!
TypeBlog PostPublishedSept. 19, 2024, 3 p.m....Bench Layout,” is below. And you’ll be able to find all published videos in our Quick Tips playlist:... -
Help Us Name Blugene’s New Virus Friend
TypeBlog PostPublishedJan. 7, 2020, 1:55 p.m....Hmmm, they need a name. And we need your help in finding a good name for Blugene’s virus friend. During... -
Meet Aavery, Blugene's New Virus Friend!
TypeBlog PostPublishedFeb. 13, 2020, 2:34 p.m....between some meaningful names…. Watch the video to find out the final four! (Note: For cases where the same... -
Hot Plasmids Podcast Episode 3: Optogenetics Tools, Improved CRISPR Base Editors, & More!
TypeBlog PostPublishedOct. 25, 2017, 1 p.m....new CRISPR base editors, DIY DNA ladders. You can find additional hot plasmids in our newsletter or on ... -
Hot Plasmids Podcast Episode 2: New RFPs, AAVs, & More
TypeBlog PostPublishedSept. 8, 2017, 7:52 p.m...., and vectors for zebrafish engineering. You can find additional hot plasmids in our newsletter or on ...