We narrowed to 806 results for: Tes
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TypeBlog PostPublishedJuly 16, 2024, 1:15 p.m...., E., Hernandez, A., Boye, S. L., Boye, S. E., Levites, Y., … Golde, T. E. (2020). Utilizing minimally...
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Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....diagonal population, this is a sign of spillover and indicates that it is necessary to apply a compensation value... -
Twenty Years of Addgene Sharing: CRISPR
TypeBlog PostPublishedApril 18, 2024, 1:15 p.m.... and the longevity of tools seen in Figure 3, indicates that the usefulness of popular CRISPR tools is... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....AntibodiesAntibodies 101: Buffers, Storage, and Conjugates Resources on Addgene.org Addgene's Antibody GuideAddgene's... -
Degrading DNA with Cascade-Cas3
TypeBlog PostPublishedFeb. 11, 2025, 2:15 p.m....unwinding of dsDNA occur simultaneously, which creates a loop in the target strand that is cut occasionally... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m....to collect your viral supernatant. Baculovirus replicates inside insect cells, and its lifecycle ends in... -
Academic vs. Industry Postdocs
TypeBlog PostPublishedNov. 13, 2018, 1:22 p.m.... companies, such as Novartis, have specific web sites for postdoc listings, so the method of posting varies... -
Anatomy of a Plasmid Page at Addgene
TypeBlog PostPublishedFeb. 4, 2016, 3:30 p.m....is an indication of popularity. A yellow flame indicates that a plasmid has been ordered more than 20 times... -
Transferable Skills Guide: Identifying Your Transferable Skills
TypeBlog PostPublishedJan. 11, 2018, 3:29 p.m....about your communication style, your potential teammates want to be assured that you will contribute openness... -
A Guide to Starting Your Own Journal Club
TypeBlog PostPublishedFeb. 6, 2020, 3 p.m....scheduling conflicts. This helped us find a time that accommodates more people. The feedback could be solicited... -
New Optimized Genome-wide CRISPRko, CRISPRi, and CRISPRa Libraries
TypeBlog PostPublishedOct. 4, 2018, 12:44 p.m....effects in human genomes (Doench et al., 2016). To test the library, they conducted genome-wide negative... -
Lentiviral Vector Uses and Overview
TypeBlog PostPublishedMay 19, 2016, 2:30 p.m....transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral... -
CRISPR Antimicrobials
TypeBlog PostPublishedMay 3, 2016, 2:30 p.m....the world is unprepared. Each year in the United States, two million people will be infected by antibiotic... -
Interview: Hodaka Fujii on enChIP, New CRISPR Tools, and More
TypeBlog PostPublishedDec. 2, 2014, 7:23 p.m....early research career. I've found that research institutes in different places have very different cultures... -
Your Lentiviral Plasmid FAQs Answered
TypeBlog PostPublishedApril 23, 2014, 1:08 p.m....envelope plasmid you use as the choice of envelope dictates the tropism of the virus. VSV-G is very common... -
How to Write a Scientific Review Article
TypeBlog PostPublishedFeb. 16, 2017, 3:30 p.m....was most productive in short bursts (even twenty minutes was enough to make some progress). For most researchers... -
Plasmids 101: Plasmid Incompatibility
TypeBlog PostPublishedMarch 31, 2020, 1:15 p.m....machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid... -
Twenty Years of Sharing: Addgene's Viral Vector Service
TypeBlog PostPublishedJuly 30, 2024, 1:15 p.m....protein tools that are activated, respectively, by substrates, light, or engineered ligands (Figure 2). Biosensors... -
Viral Vectors 101: Preparing Pooled Libraries
TypeBlog PostPublishedJuly 13, 2023, 1:15 p.m....amplification. Transformed E. coli are allowed to grow on plates or in liquid culture to amplify the library, followed... -
5 Reasons to Use Reddit for Science Communication
TypeBlog PostPublishedOct. 20, 2016, 2:30 p.m....too many to describe here, but I have over 2,000 notes which Evernote even allows me to access on my phone...