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  1. Experimenting in an Open Source lab: from CRISPR to Cats

    Type
    Blog Post
    Published
    July 23, 2015, 2:30 p.m.
    ..., in the form of workshops, presentations, and lectures. There are currently around 1,000 active makerspaces...discarded. For instance, the centrifuge in the picture had a broken controller and would not spin. It ...

  3. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...triplicate.  The nice thing about qPCR is that since it measures relative quantification - that is, since you’re...comparison of assays for accurate copy number measurement of the low-affinity Fc gamma receptor genes FCGR3A...

  4. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...here, the SMASh tag does offer a few other unique features. If your protein is not amenable to being tagged...effective it will be, given the diversity of protein structures, interacting partners, and localization. Thankfully...

  5. PhD Applications After COVID

    Type
    Blog Post
    Published
    Jan. 5, 2023, 2:15 p.m.
    ...presentation. It can help soothe your nerves and make sure you cover everything you need to during the interview...students; an online meeting can take some of that pressure off. This question and answer process can also...

  6. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...thousands of investigators studying them, how do we ensure that the research community has access to all of...24651010. 3. Ledford, Heidi. "The ras renaissance." Nature 520.7547 (2015): 278-280. PubMed PMID: 25877186...

  7. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...much more predictable than NHEJ. As seen in the figure above, short (5-25 bp) regions of homology flanking...knocking the GFP-Puro cassette into the locus (see figure below). The double-MMEJ strategy looks very similar...

  8. How to Be an Excellent Trainee

    Type
    Blog Post
    Published
    Sept. 26, 2023, 1:15 p.m.
    ... a little assistance at some point during the procedure. Be an active partner Everyone’s learning style...printed protocol, or maybe you would like to take pictures/videos of a machine set up while your mentor is...

  9. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ... two sgRNAs isn’t feasible for your experiment, ensure your sgRNA sequence has little homology to other...mediates efficient homology-directed repair and temperature-controlled genome editing. Nat. Commun. 2017 ...

  11. Transferable Skills Guide: Conflict Resolution

    Type
    Blog Post
    Published
    Jan. 4, 2022, 2:15 p.m.
    ...process that will avoid such miscommunications in the future. Assumptions: Making incorrect assumptions can...Resolving conflicts is great, but avoiding unnecessary future conflicts while giving people the tools to resolve...

  12. New Antibodies in the Lab Animation!

    Type
    Blog Post
    Published
    Nov. 19, 2024, 2:15 p.m.
    ...-based applications in your experiment...but not sure where to start or what your options are? Addgene's...

  13. Plasmids 101 eBook 4th Edition

    Type
    Blog Post
    Published
    Sept. 5, 2023, 1:15 p.m.
    ...101 covers everything from plasmids parts and nomenclature to popular cloning techniques and troubleshooting...

  15. Introducing...Addgene Quick Tips!

    Type
    Blog Post
    Published
    Sept. 19, 2024, 3 p.m.
    ...parts of every quick tip video on our TikTok! Be sure to subscribe to our YouTube channel for quick tips...

  18. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...photos and slideshows shared some of the moments we captured over the years, including an album of Addgenie...

  19. #Deckthelab Contest Winners 2017

    Type
    Blog Post
    Published
    Jan. 31, 2018, 2:44 p.m.
    ...you inspired by our 2017 #Deckthelab winners? Be sure to enter our 2018 #Deckthelab contest when the next...
Showing: 781 - 800 of 873 results