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...and could be contaminated.Learn how Addgene uses barcodes to track samples Keep track of passage numbers...

...investigators to identify specific clones with desirable properties, either initially, or using secondary...

...This will make it difficult to properly gate your desired cell population. By compensating between fluorescent...

...specific details about the individuals, each example provides a scenario where both people get something valuable...

...components into mammalian cell lines is quite broad and includes several different methods, so we've summarized...

...the site. Like Labguru, Quartzy offers software designed with scientists workflow in mind and can offer...

... to a new plate containing the mastermix which includes primers and a ddPCR supermix. Note that the supermix...

...minigenome system Minigenome systems, such as the one described here, are extremely useful research tools. In ...

...activation. Including a prey-only control also provides a baseline level of reporter activation. Vary ...

...that extra two miles which is way more fun. And despite those who may wish to tell you so – towel corners...

... plasmid delivery to a cell. In contrast, TREE provides a read out for plasmid delivery and subsequent...

...an abasic site. Like in most other base editor designs, the Cas9 nicks the non-edited strand to encourage...

...will identify a ‘scientific mentor’: someone who provides career advice and offers direction on your scientific...

...collaborators than you realize. Being at a university provides the perfect platform for practicing initiative...

... this re-inversion issue. It became possible to design FLEx vectors (Flip-excision vectors) that only ...

...difficult and time consuming. GAL4/UAS was first described in 1993 by Norbert Perrimon’s lab and has been...

...if I had planned my day extensively, my lack of desire to go sit in the tissue culture hood may have prevented...

...even better. The Thomas Kilmann Conflict Model describes 5 basic approaches to conflict resolution based...

...Biol 24, 758-766 e753 (2017). Baloban, M. et al. Designing brighter near-infrared fluorescent proteins: insights...

...with a site mutation (R11G). This site mutation destabilizes the ligase, allowing for active biotin molecules...