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  2. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...is an indication of popularity. A yellow flame indicates that a plasmid has been ordered more than 20 times...

  5. A Guide to Starting Your Own Journal Club

    Type
    Blog Post
    Published
    Feb. 6, 2020, 3 p.m.
    ...scheduling conflicts. This helped us find a time that accommodates more people. The feedback could be solicited...

  6. Lentiviral Vector Uses and Overview

    Type
    Blog Post
    Published
    May 19, 2016, 2:30 p.m.
    ...transcriptional activation from the 5’ LTR) and Rev (facilitates nuclear export of transcripts). A different viral...

  7. CRISPR Antimicrobials

    Type
    Blog Post
    Published
    May 3, 2016, 2:30 p.m.
    ...the world is unprepared. Each year in the United States, two million people will be infected by antibiotic...

  9. Your Lentiviral Plasmid FAQs Answered

    Type
    Blog Post
    Published
    April 23, 2014, 1:08 p.m.
    ...envelope plasmid you use as the choice of envelope dictates the tropism of the virus.  VSV-G is very common...

  10. How to Write a Scientific Review Article

    Type
    Blog Post
    Published
    Feb. 16, 2017, 3:30 p.m.
    ...was most productive in short bursts (even twenty minutes was enough to make some progress). For most researchers...

  12. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...machinery (Novick, 1987). In cases where one plasmid outcompetes the other, usually a smaller, higher copy plasmid...

  15. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...you want to activate, you should also be ready to test three or four guides directed towards that gene ...

  18. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ... the strong yeast promoter pTEF). As ethanol accumulates, it binds to the repressor, enabling it to bind...

  19. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ... clustering and discovery algorithms find new candidates for CRISPR proteins. A type VII is already on...

  20. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...descriptions first before entering the data. This step associates an Addgene ID with your plasmids, and adding ...
Showing: 781 - 800 of 800 results