We narrowed to 855 results for: NTS

...Accelerate Research Discoveries) Challenge provides grants for rare disease research. This year is our fourth...candidate genes in euploid iPSCs. “The BeHEARD Addgene reagents will facilitate our aims to correct X chromosome.... So far, there have been thirty GNB1 missense variants identified from 58 individuals with GNB1 disorder...biosensors and iPSC-derived cortical neurons from patients with GNB1 mutations, the lab hopes to capture ...upon stimulation. “To study cellular transduction events, we will utilize BRET-, FRET-, and intensity-based...

...turnover is radioactive pulse-chase experiments. However, these experiments are problematic because they require...annual symposium is organized by international PhD students at the Max Planck Institute for Biophysical Chemistry...MPI in Frankfurt am Main and perform similar experiments. Visualizing newly synthesized proteins with ...microscope she likes to travel, discover new restaurants, go to concerts and bake. you can contact her...

... makes polyclonal antibodies unique and what experiments you should choose them for. How polyclonal antibodies...Pre-adsorbed polyclonal antibodies are designed for experiments where antibodies raised in several different ...antibodies (which recognize other antibodies) in experiments that require multiple antibodies that each detect... For the same reason, they’re also useful in experiments where the availability of some regions of the...want to use the same positive control across experiments to be aware of signal strength. In general, polyclonal...

...based on their audience. How you talk about your experiments to your PI, your colleagues, or your non-scientist... other backgrounds at conferences or networking events. And if you’re a wet lab scientist who works frequently...formal definitions in our lexicon. We created documents and presentations, gave real life examples of ...documentation, including SOPs or project-related documents, we define new and potentially ambiguous terms... and asking can help the team identify possible points of confusion in the future. Improving cross team...

...make sure that they’ll be compatible with your instruments, each other, and your target. I love FPbase’s...reaction, can be detected using specialized imaging instruments or X-ray film and lends itself well to assays...consider a broader selection of antibodies for your experiments.  Many of the methods for antibody conjugation...If needed, you can often remove the incompatible agents by dialysis or gel filtration.    There is, of ...Conjugates: From Heterogeneous Populations to Defined Reagents. Antibodies 4:197–224. https://doi.org/10.3390...

...Stanford University. Sex and gender are critical components of biological research that are often forgotten...discovery. Tackling sex and gender in research Governments, universities, and, increasingly, corporations...researchers consider the sex of their cells when doing experiments—which can lead to failed research. For example...meant that, by the very nature of the way the experiments were performed, they did not see anything unique...of human clinical trials. And, as Janine Clayton points out, late-stage clinical studies that draw from...

...From FPs optimized for oxidizing environments to photoconvertible variants, it seems like GFP is always learning... found suitable pairs, they constructed three components: GBPa-VP16 (activation domain); GBPb-GAL4 (DNA-binding...possibility of adapting GFP lines for optogenetics experiments in specific cell populations. Listen to Our Podcast...proteins with a modular structure, constructing split variants should be relatively straightforward, but it is...

...sure that what we observe through the microscope represents reality as accurately as possible. This is especially...discover more resources for your genomic tagging experiments. Browse the Allen Institute for Cell Science ...of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...easier for you to use genomic tagging in your experiments. Many thanks to our guest bloggers from the ...

...rely on fusion proteins while others re-engineer components of Cas systems themselves. SunTag, SAM, and VPR... VPR have all shown significant improvements upon the initial dCas9-VP64 method, so there are multiple...much higher activation, dCas9-VP64 is great for experiments that requires modest gene activation. Synergistic...making it a popular method for gene activation experiments. In cases of multiplex gene regulation (activating...lines (Sajwan et al., 2019). SPH SPH combines components of SAM and SunTag activators, fusing the epitope...

...papers  You’ll need to start following relevant accounts in order to turn Twitter into a paper-finding-...paper-finding-machine. There are four general types of accounts you can follow: Scientists: Following other scientists...investing the time to read them. Organizations: Accounts for nonprofits, research organizations, and universities...research they support. Monitoring these types of accounts can help you find papers related to a particular...tip Checking the number of retweets, likes, and comments on a tweet about a paper can help you gauge how...