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We narrowed to 842 results for: NTS

Showing: 401 - 410 of 842 results

  1. Plasmids for Endogenous Gene Tagging in Human Cells

    Type
    Blog Post
    Published
    April 6, 2017, 1:02 p.m.
    ...sure that what we observe through the microscope represents reality as accurately as possible. This is especially...discover more resources for your genomic tagging experiments. Browse the Allen Institute for Cell Science ...of the gene of interest. To get the molecular components of CRISPR/Cas9 (Cas9 protein and the crRNA-tracrRNA...destabilizes the cell’s outer membrane and allows the components to physically enter the cells so they can go ...easier for you to use genomic tagging in your experiments. Many thanks to our guest bloggers from the ...

  2. Story of a SynBio Startup: RevBio's Epiphany (or Lack Thereof)

    Type
    Blog Post
    Published
    June 5, 2014, 3:32 p.m.
    ...companies that produce GMO food plants. When a press piece ran on the detector plants, we expected to see that...Facebook comment.  In our day job, we worked on plants engineered to detect explosives and rapidly indicate...that same anger. Instead, social media comments were more often along the lines of “I hate GMOs, but what...entrepreneurship, share your thoughts with us in the comments below. We’re happy to answer questions about this...

  3. Common Injection Routes in Mice

    Type
    Blog Post
    Published
    Jan. 22, 2025, 9:42 p.m.
    ...proper basic training prior to conducting mouse experiments. Some of these techniques are what we call “beginner...as this method is useful for analyzing localized events. Intradermal injections require a bit of extra ...used for delivery of viral vectors, though drug treatments are also possible. One additional requirement...through a feeding tube with a bulb on the end, which prevents accidental tears or punctures to the throat. The...overseeing your work. Good luck with your in vivo experiments! Table 1: Summary of common injection routes ...

  4. Magnetic Control of Proteins: More than a Dream

    Type
    Blog Post
    Published
    Sept. 24, 2024, 1:15 p.m.
    ... 2024 under CC-BY license. They did more experiments to see if any small molecules act as a cofactor...mutagenesis and screening, the EGFP and mScarlet variants were showing no obvious signs of improved response...deprioritize the project, Maria’s screens of AsLOV2 variants showed some significant gains! After a few more... Andrew and Maria expect that further-improved variants of MagLOV will be possible with other engineering... Anderson, E. D., Bell, A. M., Tzouanas, C. N., Avants, B. W., & Robinson, J. T. (2019). Magnetic Entropy...

  5. Of Myc and Men

    Type
    Blog Post
    Published
    Jan. 19, 2023, 2:15 p.m.
    ...also find anti-Myc [9E10] Fab fragments and single-chain variable fragments.  Abi, on the other hand...Who first started using it in protein tagging experiments? Why Myc? When did the commonly used anti-c-Myc...allow users to better troubleshoot and design experiments. It turns out that even with the most popular...Munro S, Pelham HRB (1987) A C-terminal signal prevents secretion of luminal ER proteins. Cell 48:899–...

  6. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

    Type
    Blog Post
    Published
    June 18, 2020, 1:15 p.m.
    ...they have fewer hydrophilic surfaces, require detergents or lipids to remain folded, and are often less... Generation Toolbox and commercially available reagents in three major steps - all without the need to...candidates are then reverse transcribed into cDNA fragments that are efficiently subcloned into the phagemid...research, this protocol holds promise for developing treatments for other diseases or for studying other membrane-bound... Cloning System for Characterization of Enzyme Variants. In: Methods in Molecular Biology. Humana Press...

  7. Quick Guide to Working with Drosophila Part 1: Getting Started with Flies

    Type
    Blog Post
    Published
    July 13, 2017, 2:30 p.m.
    ...method for maintaining stable lines of genetic mutants, including in situations where maintaining heterozygosity...function, while others offer fly stocks and other reagents. If you’re ever confused about the best way to...tremendous resource for the fly community. Drosophila reagents are generously shared among the research community...Bloomington, but you can search for various fly reagents on the Bloomington website as well. Additionally...Functional Genomics Resources website has in vitro reagents and tools to help you knock down, knock out, and...

  8. Addgene's Cancer Collection Pages Connect the Research Community

    Type
    Blog Post
    Published
    Sept. 6, 2016, 2:30 p.m.
    ...therapeutic agents. Nevertheless, a glimmer of light remains for this elusive target. Recent improvements in computer... circulatory and lymph systems are not ideal environments for many cell types. From proteins to pathways...Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling pathway. Its...resource! Please leave us your thoughts in the comments section below. Michael G. Lemieux is a Scientist...

  9. PITChing MMEJ as an Alternative Route for Gene Editing

    Type
    Blog Post
    Published
    Feb. 23, 2016, 3:30 p.m.
    ...doesn’t get a lot of press. However, this process accounts for a percentage of the mutations seen with TALENs...even if a repair template is present. HDR also presents a cloning dilemma - the longer the homology, the...explored. More broadly, Sakuma et al.’s use of MMEJ represents another strategy researchers can exploit for ...In organisms where HDR is downregulated, MMEJ represents another method for making targeted modifications...CRISPR Guide Pages Find CRISPR Plasmids for Your Experiments Deposit Your New Plasmid Tools with Us  ...

  10. Drew Endy Introduces the Biobrick Public Agreement Plasmid Collection

    Type
    Blog Post
    Published
    Dec. 12, 2013, 3:35 p.m.
    ...bacterial transcription and translation initiation elements. Open science with BioBricks The BioBrick Foundation's...Integrase Logic (BIL) gates collection of parts implements the basic logical functions, which we know and...out of 15 are free. Now, the BioBricks Foundation wants to develop a language for programming life. We can... prokaryotic bacteria, not in fungal systems or plants. We are really plugging into the "stuff sector"... collection of reusable, off-the-shelf genetic elements that allow you to begin to imagine designing 10...
Showing: 401 - 410 of 842 results