We narrowed to 895 results for: Ide
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TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....the SPARC system, attP sites are located on either side of a stop cassette, upstream of an effector gene...Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Nature Methods...
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Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....well-known reward chemical and neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it...Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research ... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what makes a... (this actually varies, a manager does best to identify the work style of each team member) Need challenging... -
New Viral Vectors - Winter 2025
TypeBlog PostPublishedJan. 28, 2025, noon...Bundling up for winter? The weather outside might be frightful, but browsing the 49 new preps available... vectors? Browse our in-stock viral vectors or consider our AAV Packaged on Request service, which offers... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....primary antibodies can be expensive and may not provide enough signal to visualize low-abundance targets...your primary and secondary antibodies as normal. Ideally, your secondary will only bind to your primary ... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m.... Di Ventura on Twitter, you might have seen the video of her singing about the paper recently published... transcription from the PBAD promoter. “AraC is widely used in synthetic biology, biotechnology, and almost... -
CRISPR Challenges: Standardization and Homology Directed Repair
TypeBlog PostPublishedFeb. 14, 2018, 2:36 p.m....CRISPOR, a tool to help you design, pick, and close guides for Cas9 and Cpf1. This tool supports over 250 ...Mechanisms of precise genome editing using oligonucleotide donors". Kan, Yinan, Brian Ruis, Taylor Takasugi... -
New and Upcoming Viral Vectors - December 2019
TypeBlog PostPublishedDec. 19, 2019, 2:40 p.m....sensors from the Li Lab. These GPCR based sensors provide the spatiotemporal resolution required for precise...Addgene's viral service Find viral protocols and videos Find AAVs for your research ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....sequencing projects were launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year...Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van ... -
Summer SciComm: Science Storytelling
TypeBlog PostPublishedSept. 16, 2022, 1:15 p.m....make them decide to read (or listen or watch) further. Second, the introduction needs to provide enough ... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....cells to take up protein directly? It’s not a new idea, but it comes with a few caveats. The most common...transduction strategy is to fuse a cell penetrating peptide (CPP) to a protein of interest thereby giving it... -
Early Career Researcher Toolbox: Free Tools for Science Writing
TypeBlog PostPublishedApril 28, 2021, 1:15 p.m.... schedule tools How to write a lot: A practical guide by Paula J. Silvia This book has tips for how to...suggestions. De-jargonizer The De-jargonizer can identify jargon in your writing. While it’s not possible... -
New and Upcoming Viral Vectors - September 2019
TypeBlog PostPublishedSept. 12, 2019, 1 p.m....control AAV inventory to give scientists access to a wide array of promoters and fluorophores for validating...Addgene's viral service Find viral protocols and videos Find AAVs for your research ... -
Four Factors that Differentiate the Stem Cell Field
TypeBlog PostPublishedSept. 28, 2016, 2:35 p.m....Studer discussed his work with Parkinson's Disease; Hideyuki Okano presented ground-breaking work on spinal...affordable. 4. Stem cell scientists are generous Besides some of the scientists I've already mentioned, ... -
Luminescent Imaging with Nano-lanterns
TypeBlog PostPublishedMay 25, 2017, 2:30 p.m....chemiluminescent proteins for imaging, but it has precluded widespread adoption of the technology. In 2012, Saito et... protein. This technique is very similar to the widely used FRET, but it eliminates the need for an excitation... -
Plasmids 101: Inducible Promoters
TypeBlog PostPublishedJan. 18, 2018, 2:34 p.m....? Here’s a list of some important variables to consider when choosing an inducible promoter. Experimental...Tetracycline (Tet) Inducible Systems at Addgene Cre-lox Guide and Plasmids ...