We narrowed to 906 results for: Ide
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TypeBlog PostPublishedJuly 21, 2015, 2:30 p.m....Science Careers’ myIDP tool. Using myIDP, you can identify suitable careers based on your current interests...anxiety of graduate and postdoc training. On the other side, the PI has an opportunity to check in with lab ...
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Tag Your Favorite Yeast Genes with Ease
TypeBlog PostPublishedNov. 19, 2013, 2:37 p.m.... own unique protein fusions. This collection is ideal for tandem affinity purification of protein complexes... describe a modular collection of plasmids for a wide variety of genome modifications in S. Pombe, including... -
New Norepinephrine Indicators: nLightG and nLightR
TypeBlog PostPublishedDec. 12, 2023, 2:15 p.m....antagonist. This is important because alpha2-AR can provide inhibitory feedback in response to norepinephrine...Optogenetics Plasmids Visit the Fluorescent Protein Guide Pages ... -
pSiM24: Simplifying Plant Genetic Engineering
TypeBlog PostPublishedSept. 29, 2015, 2:30 p.m....respectively, making it ideal for large gene transfer. The M24 promoter provides constitutive, high-level... -
6 Steps to Submitting a Resume That Gets Seen
TypeBlog PostPublishedJuly 9, 2015, 2:30 p.m....dramatically increase the chances of being seriously considered for an open position. Check out Joanne's Reddit...network your way to a hiring manager. Learn to confidently describe your strengths in your resume and don't... -
Future of Research Conference - Remarkable Opening Session
TypeBlog PostPublishedOct. 7, 2014, 3 p.m....Boston University last week. This fantastic city-wide effort was led by two amazing postdocs, Kristin ...about academia and alternate training needs to be provided to take advantage of all the possible science ... -
London Calling - Addgene’s New Europe Office in London
TypeBlog PostPublishedJune 10, 2014, 3:20 p.m....way to share their plasmids. Now I am on the other side of the force and as an Addgenie I will do all my...scientific discovery or invention, and sharing those ideas with others. This love of interacting with people... -
Sensing Neuronal Dopamine
TypeBlog PostPublishedJuly 30, 2019, 12:59 p.m....well-known reward chemical and neurotransmitter. But aside from DA’s famous role as the “happy hormone,” it...Addgene's viral service Find viral protocols and videos Find biosensor AAVs for your research ... -
Viral Vectors 101: Parts of the AAV Packaging Plasmid
TypeBlog PostPublishedDec. 3, 2020, 2:15 p.m....occurring AAV serotypes that have thus far been identified: AAV1-11. Nine regions of variability in the ...type tropism. Introducing changes and/or short peptides sequences into these variable loops is one method... -
Hot Plasmids - October 2022
TypeBlog PostPublishedOct. 6, 2022, 1:15 p.m....from the labs of Mingshan Xue and John Spudich identifies a novel class of light-gated potassium channelrhodopsins...KCRs), from a fungus-like protist Hyphochytrium catenoides (Govorunova et al. 2022). The authors show that... -
What's the Best Way to Elute and Store Your Plasmid DNA?
TypeBlog PostPublishedJune 4, 2020, 1:22 p.m....nuclease free water is a great option because it provides a great versatility on how to use your plasmid...Storing DNA: temperature and longevity You should consider a couple of more things when you elute your DNA... -
Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation
TypeBlog PostPublishedMay 21, 2020, 1:15 p.m....the SPARC system, attP sites are located on either side of a stop cassette, upstream of an effector gene...Cell-type-specific and projection-specific brain-wide reconstruction of single neurons. Nature Methods... -
An Addgene Summer Internship
TypeBlog PostPublishedAug. 8, 2024, 7:22 p.m....back from other summer programs. Amy took into consideration just how busy I was and ensured that my decision...concentration of a reagent during our HiFi assembly but decided to go ahead anyway. We were running on a time crunch... -
SunTag and Fluorescent Imaging
TypeBlog PostPublishedMarch 28, 2017, 2:30 p.m....SunTag, they attempted single-molecule imaging deep inside the cell, in the nucleus and cytoplasm. Again, ...Protein Empy Backbones Visit Our Fluorescent Protein Guide Page Find Luciferase Plasmids ... -
Management for Scientists: What Makes a Good Manager Anyway?
TypeBlog PostPublishedDec. 9, 2014, 2:22 p.m....Intelligence or to hone delegation skills, for example. So aside from a focus on removing obstacles, what makes a... (this actually varies, a manager does best to identify the work style of each team member) Need challenging... -
Hot Plasmids September 2018 - Optogenetics, RNA Localization, Fluorescent Protein, and Base-editing Tools
TypeBlog PostPublishedSept. 18, 2018, 1:33 p.m....through our hot plasmids articles. These articles provide brief summaries of recent plasmid deposits and ...manipulations on cells, and has potential use for a wide range of applications in cellular biology. Van ... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....sequencing projects were launched in the Quake Lab. Identifying IgnaviCas9, a hyperthermostable Cas9 This year...Cas9 Resources on Addgene.org Read our CRISPR guide Find CRISPR plasmids for your research Find links... -
What the HEK?
TypeBlog PostPublishedApril 18, 2023, 1:15 p.m....aborted fetus. HEKs have an unusual ploidy and are considered hypotriploid: most chromosomes are represented...293 is known for reproducible results. It’s also widely available, which can make for a seamless transition... -
Antibodies 101: Epitope Availability
TypeBlog PostPublishedJan. 11, 2022, 2 p.m....available for binding change as well. If this change hides the epitope that the antibodies used to recognize...bind to the epitopes that are available on the outside of the cell. The same protein may have different... -
A New Optogenetic Tool Based on AraC Controls Gene Expression with Blue Light
TypeBlog PostPublishedJune 17, 2021, 1:17 p.m.... Di Ventura on Twitter, you might have seen the video of her singing about the paper recently published... transcription from the PBAD promoter. “AraC is widely used in synthetic biology, biotechnology, and almost...