We narrowed to 848 results for: LEA

1. Addgene: The First Twenty Years

   Type

   Blog Post

   Published

   Dec. 19, 2024, 2:15 p.m.

   ...Award, which recognizes researchers who have at least one plasmid deposited at Addgene that has been distributed...

2. Choosing the B(right)est Fluorescent Protein: Aggregation Tendency

   Type

   Blog Post

   Published

   June 15, 2017, 2:30 p.m.

   ...Your Fluorescent Proteins for Multicolor Imaging Learn about Fluorescent Protein Biosensors Additional...

3. CRISPRainbow and Genome Visualization

   Type

   Blog Post

   Published

   Feb. 28, 2017, 3:30 p.m.

   ...Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...

4. Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number

   Type

   Blog Post

   Published

   Sept. 4, 2018, 12:53 p.m.

   ... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ...

5. Antibodies 101: Polyclonal Antibodies

   Type

   Blog Post

   Published

   July 14, 2021, 1:15 p.m.

   ...of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based...

6. Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options

   Type

   Blog Post

   Published

   May 11, 2021, 1:15 p.m.

   ...its localization in the membrane. The lesson we learned from these studies is that the tag protein can ...

7. Troubleshooting Your Plasmid Cloning Experiment

   Type

   Blog Post

   Published

   Sept. 24, 2019, 12:55 p.m.

   ...for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ...

8. It’s Bioelectric! An Exciting Interdisciplinary Field of Research

   Type

   Blog Post

   Published

   Oct. 29, 2019, 2:52 p.m.

   ...an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that...

9. CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt

   Type

   Blog Post

   Published

   April 22, 2015, 2:06 p.m.

   ...through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs...

10. Design Tips for Prime Editing

    Type

    Blog Post

    Published

    Jan. 23, 2025, 2:15 p.m.

    ...strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...

11. CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!

    Type

    Blog Post

    Published

    Oct. 6, 2020, 1:15 p.m.

    ... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the...

12. How to Prepare for an Industry Interview

    Type

    Blog Post

    Published

    July 9, 2020, 1:15 p.m.

    ... interview. Each interview is an opportunity to learn something and then apply it to the next interview...

13. Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)

    Type

    Blog Post

    Published

    Aug. 24, 2021, 1:15 p.m.

    ...03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ...

14. Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them

    Type

    Blog Post

    Published

    May 4, 2021, 1:15 p.m.

    ...Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ...

15. Hot Plasmids and Viral Preps - January 2021

    Type

    Blog Post

    Published

    Jan. 5, 2021, 2:15 p.m.

    ...ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in this ...

16. Hot Plasmids: Winter 2025

    Type

    Blog Post

    Published

    March 11, 2025, 1:15 p.m.

    ... your research! If they do (or if they don't!), please consider sharing your experience with other researchers...

17. The AAVantages of AAV in CRISPR Screens

    Type

    Blog Post

    Published

    Feb. 4, 2025, 2:15 p.m.

    ...be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified...

18. Common Injection Routes in Mice

    Type

    Blog Post

    Published

    Jan. 22, 2025, 9:42 p.m.

    ...preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge...

19. Production of Virus in Insect Versus Mammalian Cells

    Type

    Blog Post

    Published

    Nov. 5, 2024, 8:32 p.m.

    ... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach...

20. Antibodies 101: Flow Compensation

    Type

    Blog Post

    Published

    May 16, 2024, 1:15 p.m.

    ...compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells...