We narrowed to 845 results for: LEA
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TypeBlog PostPublishedFeb. 28, 2017, 3:30 p.m....Read More about Fluorescent Protein-Based Tools Learn Some of the Pitfalls When Using Fluorescent Proteins...
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Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....of antibody for research, monoclonal antibodies Learn about the advantages of using recombinant plasmid-based... -
Tagging Optogenetics and Chemogenetics Receptors: Fluorescent Proteins and Other Options
TypeBlog PostPublishedMay 11, 2021, 1:15 p.m....its localization in the membrane. The lesson we learned from these studies is that the tag protein can ... -
It’s Bioelectric! An Exciting Interdisciplinary Field of Research
TypeBlog PostPublishedOct. 29, 2019, 2:52 p.m....an otherwise normal genomic background, can even lead to the formation of whole eyes from cell types that... -
Troubleshooting Your Plasmid Cloning Experiment
TypeBlog PostPublishedSept. 24, 2019, 12:55 p.m....for digestion. PCR Design primers that overlap at least 24 bp with the template and if the sequence has ... -
Stabilized Bacterial Promoters: Constant Gene Expression at any Copy Number
TypeBlog PostPublishedSept. 4, 2018, 12:53 p.m.... IPTG concentrations. Higher IPTG concentration leads to higher RepA expression which results in high ... -
CRISPR Meets Synthetic Biology: A Conversation with MIT’s Christopher Voigt
TypeBlog PostPublishedApril 22, 2015, 2:06 p.m....through Addgene, and where all of it is likely to lead us in the future. Building circuits from CRISPRs... -
Design Tips for Prime Editing
TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m.... interview. Each interview is an opportunity to learn something and then apply it to the next interview... -
Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....ever like to write about a recent plasmid deposit please sign up here. Here's what you'll find in this ... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m.... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m.... your research! If they do (or if they don't!), please consider sharing your experience with other researchers... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....the fluorescent signature upon antibody binding, leading to discrepancies during unmixing. Additionally,... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....science happens in a vacuum! Munro and Pelham learned of these new antibodies and identified Myc1-9E10...