We narrowed to 839 results for: LEA
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TypeBlog PostPublishedJan. 23, 2025, 2:15 p.m....strand before heteroduplex resolution, which can lead to indels (Anzalone et al., 2019). Figure...
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Antibodies 101: ELISA (Enzyme-linked Immunosorbent Assay)
TypeBlog PostPublishedAug. 24, 2021, 1:15 p.m....03239-6 Additional resources on the Addgene blog Learn about the advantages and disadvantages of using ... -
Plasmid-based Recombinant Monoclonal Antibodies: What They Are and Why You Should Be Excited About Them
TypeBlog PostPublishedMay 4, 2021, 1:15 p.m....Antibodies 101: Introduction to Antibodies blog post Learn more about hybridomas Watch our Antibodies 101: ... -
Hot Plasmids and Viral Preps - January 2021
TypeBlog PostPublishedJan. 5, 2021, 2:15 p.m....ever like to write about a recent plasmid deposit please sign up here. Here's what you'll find in this ... -
How to Prepare for an Industry Interview
TypeBlog PostPublishedJuly 9, 2020, 1:15 p.m.... interview. Each interview is an opportunity to learn something and then apply it to the next interview... -
CRISPR Activators: A Comparison Between dCas9-VP64, SAM, SunTag, VPR, and More!
TypeBlog PostPublishedOct. 6, 2020, 1:15 p.m.... dCas9, a CRISPR protein variant lacking its endonuclease ability, to bind to genes without editing the... -
Hot Plasmids: Winter 2025
TypeBlog PostPublishedMarch 11, 2025, 1:15 p.m.... your research! If they do (or if they don't!), please consider sharing your experience with other researchers... -
The AAVantages of AAV in CRISPR Screens
TypeBlog PostPublishedFeb. 4, 2025, 2:15 p.m....be packaged in a variety of capsids. To date, at least 12 different capsids, or serotypes, have been identified... -
Common Injection Routes in Mice
TypeBlog PostPublishedJan. 22, 2025, 9:42 p.m....preferences. It sounds complicated but has a pretty low learning curve! Restraint: scruffing Location: throat Gauge... -
Production of Virus in Insect Versus Mammalian Cells
TypeBlog PostPublishedNov. 5, 2024, 8:32 p.m.... cells, and its lifecycle ends in cell lysis, releasing the virus to the supernatant. The virus will reach... -
Antibodies 101: Flow Compensation
TypeBlog PostPublishedMay 16, 2024, 1:15 p.m....compensation values later. This can be helpful as you're learning! Ashlyn has a research background in CD8+ T cells... -
Antibodies 101: Conventional vs Spectral Flow Cytometry
TypeBlog PostPublishedAug. 3, 2023, 1:15 p.m....the fluorescent signature upon antibody binding, leading to discrepancies during unmixing. Additionally,... -
Of Myc and Men
TypeBlog PostPublishedJan. 19, 2023, 2:15 p.m....science happens in a vacuum! Munro and Pelham learned of these new antibodies and identified Myc1-9E10... -
Polymerase Chain Reaction Overview and Applications
TypeBlog PostPublishedAug. 10, 2021, 1:15 p.m....currently uses ddPCR for AAV titrating. You can learn all about ddPCR for AAV titration and find helpful... -
CrispyCrunch: High-throughput Design and Analysis of CRISPR+HDR Experiments
TypeBlog PostPublishedFeb. 7, 2019, 2:16 p.m....the Addgene blog Read our CRISPR 101 blog posts Learn about some considerations when designing gRNAs Find... -
Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More
TypeBlog PostPublishedJune 12, 2014, 3:30 p.m.... have your parts. That was the motivation for releasing a large parts kit. Once you have so many parts... -
Tips for Using BLAST to Verify Plasmids
TypeBlog PostPublishedMay 29, 2014, 1:29 p.m....: An Inside Look at NGS Plasmid Quality Control Learn about our Snapgene-powered plasmid maps. Resources... -
Antibodies 101: Producing Recombinant Antibodies
TypeBlog PostPublishedAug. 13, 2024, 1:15 p.m....quantified, and validated, it’s ready for use! You can learn more about common antibody applications in our Antibodies... -
Plasmids 101: Choosing an Antibiotic Resistance Gene
TypeBlog PostPublishedOct. 10, 2023, 1 p.m....gene does not fully suppress Zeocin-induced DNA cleavage in human cells. Mutagenesis, 20(2), 111–114. https... -
Getting to Know Expansion Microscopy
TypeBlog PostPublishedJune 20, 2023, 1:15 p.m....This limitation may be the both most obvious and least flexible one. Tissue expansion is not compatible...