We narrowed to 813 results for: TIM

1. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

   Type

   Blog Post

   Published

   Dec. 14, 2017, 2:08 p.m.

   ...episomal vector system generated by the group is an optimized mixture of three episomal plasmids (Table 1) encoding...

2. Plasmids 101: Gateway Cloning

   Type

   Blog Post

   Published

   Jan. 12, 2017, 3:30 p.m.

   ...that will work well with a given set of resources, time, and experimental needs. Since its invention in ...

3. CRISPR Activation: A Practical Guide

   Type

   Blog Post

   Published

   Aug. 18, 2016, 2:30 p.m.

   ...appropriate isoform(s) of a given gene, as, many times, the isoform(s) common to a particular cell type...

4. Adeno-associated Viruses (AAVs) for Genome Editing

   Type

   Blog Post

   Published

   March 27, 2018, 1:32 p.m.

   ...in vivo applications.  Third, even in the most optimized tissue-culture based system, the efficiency of...

5. Plasmids 101: Expressing shRNAs for RNA Interference

   Type

   Blog Post

   Published

   June 17, 2025, 1:15 p.m.

   ...dedicated field of study for improving these tools. Next time you need to knock down a gene, maybe you’ll consider...

6. Antibodies 101: Beyond Surface Labeling

   Type

   Blog Post

   Published

   June 27, 2024, 1:15 p.m.

   ...using biorender.com.   Intracellular labeling Oftentimes, a marker of interest is not located on the cell...

7. Plasmids 101: Biotinylation

   Type

   Blog Post

   Published

   Nov. 15, 2018, 1:50 p.m.

   ...(McInnes et al., 1990). Photobiotinylation is sometimes easier and less expensive than other enzymatic...

8. Targeting HIV-1 with CRISPR: Shock and Kill or Cut it Out?

   Type

   Blog Post

   Published

   May 10, 2016, 2:30 p.m.

   ...genomes will need to be sequenced to determine the optimal gRNAs for either “shock and kill” or viral excision...

9. Plasmids 101: Common Lab E. coli Strains

   Type

   Blog Post

   Published

   Nov. 7, 2014, 2:56 p.m.

   ...designing your plasmid – you carefully selected the optimal promoter for your gene of interest, painstakingly...

10. A Control for All Seasons

    Type

    Blog Post

    Published

    Oct. 5, 2023, 1:15 p.m.

    ..., you could repeat the tau western blot several times with the same samples and compare the results across...

11. Generating Mouse Models Using CRISPR/Cas9

    Type

    Blog Post

    Published

    July 12, 2016, 2:30 p.m.

    ...CRISPR is easier than more conventional methods. Time and money savings come from the fact that CRISPR...

12. PiggyBac-ing Through the Genome Editing Field

    Type

    Blog Post

    Published

    May 31, 2016, 3:30 p.m.

    ... technology using a site-specific nuclease to stimulate homologous recombination and Footprint-Free™ removal...

13. CRISPR 101: Cytosine and Adenine Base Editors

    Type

    Blog Post

    Published

    Feb. 13, 2025, 2:15 p.m.

    ...cytidine and adenine base editors by expression optimization and ancestral reconstruction. Nature Biotechnology...