We narrowed to 816 results for: ache
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TypeBlog PostPublishedJuly 25, 2019, 12:03 p.m.... a virus and the plant's post-transcriptional machinery to silence genes of interest (Unver and Budak,...
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Hot Plasmids Spring 2024
TypeBlog PostPublishedMay 23, 2024, 1:15 p.m....Paul Blainey’s lab has developed a multiplexing approach to Cas9-based pooled CRISPR screens called CROPseq-multi... -
CRISPR 101: Targeting RNA with Cas13a (C2c2)
TypeBlog PostPublishedSept. 21, 2017, 2:07 p.m....is in stark contrast to Cas9, which requires that each DNA target have high sequence identity to the spacer... -
Antibiotic Resistance: An Old Solution but a New Problem
TypeBlog PostPublishedNov. 10, 2016, 3:30 p.m....rotating antibiotic treatments, so that exposure to each antibiotic is minimized, while the infection is ... -
Five Popular Model Organisms
TypeBlog PostPublishedApril 11, 2019, 12:39 p.m....al. "Efficient gene transfer in C. elegans: extrachromosomal maintenance and integration of transforming... -
Quick Guide to Near-Infrared Fluorescent Proteins
TypeBlog PostPublishedOct. 5, 2021, 1:15 p.m.... A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters. Nat ... -
Antibodies 101: Single Chain Fragment Variables (scFvs)
TypeBlog PostPublishedJune 3, 2021, 1:15 p.m....laborious. To avoid this, one or more of these approaches are often used for scFv expression in bacteria... -
Viral Vectors 101: Integrase-Deficient Lentiviral Vectors
TypeBlog PostPublishedMay 13, 2025, 1:15 p.m...., and the integration site will be different for each transduced cell, there’s no telling which cells ... -
New Optogenetic Tools for Cytoskeleton and Membrane Control
TypeBlog PostPublishedSept. 28, 2023, 1:15 p.m.... stimulate and study these physical processes. Joachim Goedhart and colleagues in the Gadella Lab devised... -
An Integrin Antibody Toolkit from IPI
TypeBlog PostPublishedMarch 28, 2024, 1:15 p.m....ligand-binding pocket, their sheer size impeding ligand attachment. The five other IPI rAbs bind RGD-binding integrins... -
3 Tips to Improve HDR Efficiency for CRISPR Editing in Human Cells
TypeBlog PostPublishedSept. 5, 2017, 1:58 p.m.... resulting in cells that use one or the other on each target site. Figure 3: Optimal cut site... -
Multiple Plasmids at a Low Price: Kits from Addgene
TypeBlog PostPublishedSept. 14, 2016, 2:30 p.m....kit consists of a 48 plasmid adapter library, with each plasmid carrying a unique barcoded Illumina-M13F... -
10 Steps to a Perfect Science Talk
TypeBlog PostPublishedAug. 23, 2016, 2:30 p.m....the particular audience and venue before tailoring each presentation appropriately. Find out who will be... -
Plasmids 101: Repressible Promoters
TypeBlog PostPublishedDec. 20, 2022, 2:15 p.m.... are orthogonal, meaning that they do not affect each other. For example, the GAL4 transcription factor... -
Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)
TypeBlog PostPublishedMarch 7, 2017, 3:30 p.m....analyses, and give what I think are the main tips for each step of performing and analyzing a gene edit using... -
Cancer and the Immune System: Deciphering the Relationship
TypeBlog PostPublishedMarch 14, 2017, 2:30 p.m....objective of efficient immunotherapy must be to reach a flexible personalized treatment, and not universal...