We narrowed to 884 results for: ica;

...CRISPR array. The CRISPR array is composed of identical repeat sequences and variable spacer sequences...simplifying the CRISPR-Cas9 system for laboratory applications. At Addgene, we sometimes refer to the programmable...

...Their work involves coordination and can require technical mastery. Scientists need to be doers for a long...in the way of the doers and by ensuring good communication. A manager must both hire and fire to make sure...

...be a time when you need to speak publicly to communicate your work. One of my 10 tips for giving a great...the board of a professional society, solved a technical problem, had an alter ego as a mad scientist for...

...contains the proteins needed for the virus to replicate,  and the transfer plasmid which contains the ... sites in the ITRs. Regular Sanger sequencing typically fails to sequence through ITRs due to their secondary...

...Therapy. Existing methods for AAV quality control Historically, AAV vector QC has been sparse including only...that could be used as a template for downstream applications (Berns, 1972). Using qPCR and restriction enzyme...

...of autophagic substrates inside the lysosome. Historically, methods for studying autophagy focused on counting... autophagic activity, and are a more reliable indicator of autophagic activity. The aim of this post is...

...gene encodes a sodium-potassium pump expressed specifically in neurons of the brain. McSweeney says they...models carrying different SOX10 mutations. More specifically, they’ve proposed to use plasmids with modified...

...positions posted on recruitment websites, this job specifically looked for a researcher with 3 years+ of cancer...on the marketing or business background of the applicant. However, although this position did look for ...

...FiercePharma), or sign up to be alerted about their new publications via Pubmed, so you can congratulate them when...really understood what I was going through. (Parenthetically, when I called the lab to ask my benchmate ...

...one session to another,” Bachle says. “Now, the physical location didn’t matter. I didn’t have to consider...conference is over on the website or in the app. Technical difficulties? Attending a conference virtually...

...Christopher Harvey’s lab. According to their publication (Chettih & Harvey, 2019), “this localization ...Recombinase AAV Targeting and manipulating specific, genetically-defined neuronal populations with intersectional...

...pipetting experience. However, after a few months of dedicated time at BosLab, he became our bacterial transformation... for discovering novel therapeutics. Dave is a chemical biologist with a Ph.D. from UNC-Chapel Hill and...

... dialogue and community among participants, clarification - new and useful information, and invigoration...be the representative in the meeting and then communicate outcomes to the rest of their team. Here's a ...

...end joining (NHEJ). In these reporters, editing typically results in a change in the fluorescent readout...stimulated homology-directed repair. Journal of Biological Chemistry 292:6148–6162 . https://doi.org/10.1074...

...as help our students succeed whether that be academically or on the employment front. Paratore: I work...& Morning. Karen was a founding member of Mass Medical Angels, a venture mentor at MIT and served on the...

... the following, which are later printed for my physical lab notebook, along with any figures generated.... She is particularly interested in science communication and epigenetics. Follow her on twitter @kc_palozola...

...plasmid. You should also verify the strain and the efficacy of your competent cells (when they get old, they...constructs. His work was acknowledged in hundreds of publications including Cell, Nature, and other journals. ...

...a method that uses loop-mediated isothermal amplification after reverse transcription (RT-LAMP) and a ...bidirectional CRISPR screens identify mucins as critical host factors modulating SARS-CoV-2 infection. ...

... tool to identify a protein of interest in a complicated solution like cell lysate. But they can be a ...reacts with antibodies, so thorough washing is critical. Assess the stripping Now it’s time to check ...

...downstream of the gene to be transcribed, and typically occur directly after any 3’ regulatory elements...rho-dependent and rho-independent. Rho factor is a helicase which assists RNA polymerase in the termination...