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...of transposons. Insertion of the mini-transposon can occur in two different orientations, but is strongly...encodes the mini-transposon. pEffector and pDonor can also be combined in single-plasmid INTEGRATE (pSPIN...was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical...guest blogger Leo Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical...

...Endotoxin Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product... after negative staining. Empty vector particles can be identified after negative staining and appear ...

...HDR). Cpf1 also expands the number of sites that can be targeted by CRISPR to AT-rich regions or AT-rich... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche et al. found two that display robust... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

...to build relationships with their colleagues. It can be easy to get caught up in the minutia of the workday... that minutia gets much more manageable when you can enjoy the people you spend 40+ hours with each week...culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the question...is certainly not the truth (partially because I can’t whistle). No, we’re not all BFFs. Yes, sometimes...

... of the laser sheet and using multi-view imaging can provide enhanced resolution (8,9).  Thus, LSFM is...adeno-associated virus)-based fluorescent labeling methods that can be used with 3DISCO to image non-transgenic animals...to photobleaching.  Furthermore, point-by-point scanning along the entire specimen makes imaging too slow...photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is performed... milliseconds to acquire one image, thereby significantly reducing the time it takes to image through ...Similar to light microscopy, there have been significant advances in tissue clearing techniques during...

...antibody joining us on our scientific adventures.  You can submit your name suggestions here or by using the...best?     But between these options, we simply can't decide! Abi, Lin, or Blottie? Which name best fits...we’re looking for a gender neutral name (and it can’t hurt if it’s also a great pun.) Submissions are ...

... have a collection of snowman figurines that you can stage in a lab diorama. Or maybe you want to create...

...serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted viral vector reports...

...Plasmid articles with each of our newsletters. You can find all of these articles on the Hot Plasmids webpage...

...Listen to Additional Addgene Podcasts Learn How You Can Contribute to the Addgene Blog Learn about Addgene...

...serotype. We've also added two viral vectors that can constitutively express JEDI-2P, deposited by the ...

...Instagram     Eppi the red nosed Reindeer! 🦌🦌 as you can see lots of work always gets done this time of year...

...creative ideas applicants will propose for plasmids from the Addgene community and hope we can do even more...

...From our humble beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally...

...reflecting on a question of impact that only you can answer: How would you like to measure yours? More...

... amino acids, can also be packaged into AAV. NmeCas9 has the added advantage that it can be turned off...

...recently that desired genome-editing events by HDR can be generated more efficiently by (1) improving editing...template containing blocking and intended mutations can be equally mixed with another ssODN template that...

...synthetic biology techniques in biology research as can already be seen with things like DNA synthesis, Golden...

... sure scientists all over the world know that we can help with plasmid sharing! Judging by how many prizes...

...they need for their research, show labs how they can benefit from depositing their constructs to the repository...