We narrowed to 911 results for: CAN

..., this method is fast! These editing frequencies can be achieved with a 30-minute incubation, even with...it quickly saw wide interest, its dimeric nature can interfere with some applications. Several labs have...

...lines.   dCas9-VP64 Description CRISPR activation can occur through fusing dCas9 with VP64, a strong transcriptional...sequences, causing targeted gene regulation. This can be used to activate transcription during either initiation...themselves. SunTag, SAM, and VPR have all shown significant improvements upon the initial dCas9-VP64 method...Performance Generally, VPR is found to have significantly higher activation levels than the initial dCas9...

... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...Read About CRISPR Competitor NgAgo Learn How You Can Use piggyBac Vectors in Genome Editing Resources...

...different fluorescent proteins. Visualizing fluorescence can be challenging in plants because of the auto-fluorescence...prejudice about it. They just start and go ahead. I can see science speeding up. Addgene: Are there other... be equally high as for those who go to work in cancer biology.  References: A Golden Gate Modular Cloning...

...fashion. Addgenies swapping out printer cartridges can place the spent cartridge in the large cardboard box ...requires a significant energy expenditure here at Addgene. These are critical systems that cannot be removed...and recycling in the event the object in question cannot be washed or reused/repurposed in some fashion....

...consistently contribute to our collection so that we can offer the scientific community the latest and greatest...

...Endotoxin Endotoxin contamination in vector preparations can alter the immunogenic properties of the final product... after negative staining. Empty vector particles can be identified after negative staining and appear ...

...of transposons. Insertion of the mini-transposon can occur in two different orientations, but is strongly...encodes the mini-transposon. pEffector and pDonor can also be combined in single-plasmid INTEGRATE (pSPIN...was contributed by guest blogger, Leo Vo, a PhD candidate in the Sternberg Lab at Columbia University Medical...guest blogger Leo Vo! Leo Vo is currently a PhD candidate in the Sternberg Lab at Columbia University Medical...

...HDR). Cpf1 also expands the number of sites that can be targeted by CRISPR to AT-rich regions or AT-rich... so, with Kleinstiver et al. reporting that Cpf1 can tolerate mismatches at gRNA positions 1, 8, 9, and...activity in mammalian cells. From 16 diverse Cpf1 candidates, Zetsche et al. found two that display robust... Direct synthesis of these crRNAs should be significantly cheaper than that of the ~100 nt crRNA/tracrRNA...

...to build relationships with their colleagues. It can be easy to get caught up in the minutia of the workday... that minutia gets much more manageable when you can enjoy the people you spend 40+ hours with each week...culture here at Addgene has remained paramount. Candidates who interview at Addgene frequently ask the question...is certainly not the truth (partially because I can’t whistle). No, we’re not all BFFs. Yes, sometimes...

... of the laser sheet and using multi-view imaging can provide enhanced resolution (8,9).  Thus, LSFM is...adeno-associated virus)-based fluorescent labeling methods that can be used with 3DISCO to image non-transgenic animals...to photobleaching.  Furthermore, point-by-point scanning along the entire specimen makes imaging too slow...photo-bleaching or photo-damage than in conventional laser scanning microscopy . Since image acquisition is performed... milliseconds to acquire one image, thereby significantly reducing the time it takes to image through ...Similar to light microscopy, there have been significant advances in tissue clearing techniques during...

...serotypes. Not sure what to request? Addgene's Data Hub can help, with over 100 user-submitted viral vector reports...

...Plasmid articles with each of our newsletters. You can find all of these articles on the Hot Plasmids webpage...

...antibody joining us on our scientific adventures.  You can submit your name suggestions here or by using the...best?     But between these options, we simply can't decide! Abi, Lin, or Blottie? Which name best fits...we’re looking for a gender neutral name (and it can’t hurt if it’s also a great pun.) Submissions are ...

... have a collection of snowman figurines that you can stage in a lab diorama. Or maybe you want to create...

...Listen to Additional Addgene Podcasts Learn How You Can Contribute to the Addgene Blog Learn about Addgene...

...serotype. We've also added two viral vectors that can constitutively express JEDI-2P, deposited by the ...

...Instagram     Eppi the red nosed Reindeer! 🦌🦌 as you can see lots of work always gets done this time of year...

...creative ideas applicants will propose for plasmids from the Addgene community and hope we can do even more...

...From our humble beginnings in 2004 to today, you can see how plasmid distribution has grown. Additionally...