We narrowed to 860 results for: PLE;

...Overview of Cas9 nickase We’ll use SpCas9 nickases as examples for this post. The D10A mutation inactivates the...

...Snapgene desktop software allows us to quickly align multiple sequences at the same time. This means we can ...

...2017). Type I CAST systems rely on the Cascade complex minus its nuclease component, Cas3, while Type ...

...spontaneous swimming and escape reflex. These traits, coupled with short generation times and high fecundity,...

...your search to particular types of images. For example, by selecting “Tools” and then clicking the “Usage...

...packaging (no coated tissue culture plates, for example). Check the link for more details on what they ...

...editing. See figure 4 in Harrington et al for more examples of GeoCas9’s editing activity in HEK293’s. GeoCas9...

...gene of interest is cloned into/inactivates or completely removes (in the case of Gateway cloning) one ...

... specific activity for each reagent batch. To supplement this, Addgene will be creating a crowdsourced...

...Medical School (HMS) where he leads a lab of 25 people. He also teaches at HMS and has been involved in...

... 8 amino acid tag (DYKDDDDK) that can be added multiple times (often 3X FLAG, but can be more!) to a protein...

... scientists fused them to sequences called complementarity determining regions (CDRs, or loops) of an ...

...agreements slow down your research; provide specific examples if possible Tell them about the Golden Stopwatch...

...contribution to the community because of..." This first couple of sentences - even if written in a clumsy language...

...fluorescent protein oligomerization Seeing red: simple GFP photoconversion Light sheet fluorescence microscopy...

...synthetic vs. natural gene are essential and complementary tools in a time where biological engineering...

...might make it difficult to use NgAgo to disrupt multiple targets. Are you using NgAgo? Let us know how ...

...and a priority list of experiments you hope to complete. Share this plan with your host prior to arrival...

...1990s when they abandoned the system. Since then, multiple biotech and pharmaceutical companies (along with...

... validated the serotonin sensor with proof-of-principle experiments that replicate current knowledge, ...