We narrowed to 863 results for: TOR;
-
TypeBlog PostPublishedMay 11, 2023, 1:15 p.m....was originally derived from jellyfish (Aequorea victoria) and can serve as an epitope tag as well as a ...
-
Donations from Addgene to Yield Answers for Rare Disease Researchers
TypeBlog PostPublishedSept. 15, 2015, 2:30 p.m....rare diseases,” said Claudia OuYang, BeHEARD Co-Director in a release. The research teams studying four... -
Even more elegant: Single injection CRISPR/Cas9 in C. elegans
TypeBlog PostPublishedJuly 7, 2015, 3:36 p.m.... arms for your gene of interest into the FP-SEC vector using Gibson assembly. This construct will serve... -
Another Pathway into Cells: iTOP
TypeBlog PostPublishedJune 23, 2015, 8:37 p.m....limitations of packaging size associated with viral vectors. For these reasons, iTOP, especially in tandem ... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....radio reporter challenge, and code.org has coding tutorials for all ages. Organize your data and prepare ... -
FlipGFP, a novel fluorescence protease reporter to study apoptosis
TypeBlog PostPublishedMay 21, 2019, 12:10 p.m....to small fluorescence changes of the donor and acceptor after proteolytic cleavage. Secondly, fluorescence... -
Summer SciComm Series: A PhD in Science Communication
TypeBlog PostPublishedAug. 23, 2022, 1:15 p.m....poring over my grandparents’ medical textbooks by torchlight under the covers. In high school, I went to all... -
How to Lead a Great Meeting
TypeBlog PostPublishedFeb. 9, 2016, 3:30 p.m.... Get Management Advice from Addgene Executive Director Joanne Kamens FInd Tips on Picking a Research ... -
Components of CRISPR/Cas9
TypeBlog PostPublishedFeb. 2, 2016, 5 p.m.... to modify the desired genomic locus. Some historical notes CRISPR arrays in bacterial genomes consist... -
Quick Guide to Working with Drosophila Part 2: Controlling Gene Expression in Flies with Gal4/UAS
TypeBlog PostPublishedJuly 21, 2017, 12:48 p.m....turn genes on or off. Gal4 is a transcriptional activator that binds to UAS enhancer sequences found in ... -
Building and Validating Recombinant Antibodies: A Case Study
TypeBlog PostPublishedApril 5, 2022, 1:20 p.m....secondary antibodies are commonly used in research laboratories to detect the different subclasses, but there... -
Flow Cytometry Readouts: Yes, No, and Everything in Between
TypeBlog PostPublishedAug. 20, 2024, 1:15 p.m....Purification Protocol Video rAbs in the Addgene repository... -
Plasmids 101: Methylation and Restriction Enzymes
TypeBlog PostPublishedJune 30, 2016, 2:30 p.m....these strains should not be used for long term storage. Additional Resources Review of restriction modification... -
A Practical Approach to Choosing the B(right)est Fluorescent Protein
TypeBlog PostPublishedJune 1, 2017, 2:30 p.m....excitation wavelength, available emission filters, and detector sensitivity) and the biological system (temperature... -
CasPEDIA: A Functional Classification of Cas Enzymes
TypeBlog PostPublishedJan. 25, 2024, 2:15 p.m.... any other required non-CRISPR-associated host factors required for CRISPR processing. For each enzyme... -
Sharing is Caring: But How to Distribute Open Hardware?
TypeBlog PostPublishedFeb. 28, 2023, 2:15 p.m.... in science brings stronger networking across laboratory and disciplinary boundaries and can allow for... -
How to Make Friends and Meet People at a Scientific Conference
TypeBlog PostPublishedAug. 7, 2014, 1:58 p.m....You never know when you will meet a potential collaborator Some of these people you meet will review your... -
Transferable Skills Guide: Problem Solving
TypeBlog PostPublishedNov. 30, 2021, 2:15 p.m.... you can start by paying attention to how your mentors reach solutions and ask them to explain their logic... -
Antibodies 101: Polyclonal Antibodies
TypeBlog PostPublishedJuly 14, 2021, 1:15 p.m....Fishman JB, Berg EA (2019) Antibody Purification and Storage. Cold Spring Harb Protoc 2019:pdb.top099101. https... -
Antibodies 101: Fab Fragments
TypeBlog PostPublishedSept. 5, 2024, 1:15 p.m....secondary antibodies you already have in your refrigerator will not recognize a Fab fragment. In order ...