Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 842 results for: NTS

Showing: 421 - 430 of 842 results

  1. Antibodies 101: Immunoprecipitation

    Type
    Blog Post
    Published
    Dec. 7, 2021, 2:15 p.m.
    ...approaches allow for optimized strategies for differing amounts of target proteins or antibody bonding strengths...target protein or if the target is present in low amounts. In this method, the free antibody is incubated...magnetic beads with commercially available chemical agents. Directly conjugating the primary antibody has ... a non-denaturing buffer containing non-ionic detergents, such as NP-40 or triton-X, to preserve native...than endogenous proteins. Data obtained from experiments using a tagged protein may not be translatable...

  2. Plasmids 101: Shuttle Vectors

    Type
    Blog Post
    Published
    May 20, 2025, 1:15 p.m.
    ...’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would prefer...resistance gene, so it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end...Auxotrophic selection is especially common in yeast experiments because yeast tend to spontaneously develop resistance... but it isn’t common for simple transfection experiments. Reporters Finally, in some cases a researcher...gene they simply can’t turn on! Wait, what even counts as a shuttle vector? Are viral vectors shuttle ...

  3. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...produce lower amounts of protein, which can be useful if the product is toxic in high amounts. These plasmids...appropriate time—for example, the cell must have enough nutrients available to complete the entire round of replication... are efficient for the host, as no additional components are necessary. Iterons are not the only tool ...sequence forming this pseudoknot, so its binding prevents the mRNA from folding and blocks RepZ translation...

  4. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    ...recombination and avoid separation of the multiplexed elements. The team designed sgRNAs that include an internal...by this tool as well as additional potential improvements to the editor. Find PE7 plasmids here!   ...in the rhodopsin domain conferred several new improvements. The new variant has increased signal-to-noise...voltage indicator Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure...trans-splicing. By splitting a large gene into smaller fragments, delivering them by individual AAVs, and then ...

  5. Plasmids 101: Broad Host Range Plasmids

    Type
    Blog Post
    Published
    April 11, 2024, 1:15 p.m.
    ...origin of replication (ori) contains structural elements that are versatile and adaptable. Variation in...bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number and stability...differentiated from one another. Based on their structural elements, oris interact with various RNA polymerases and...Host range depends on whether the host has the components to recognize the ori and allow for replication...consist of a plasmid’s ori and all of its control elements, from broad host range plasmids can be used to...

  6. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...Cre-lox or one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors –... – inversion only once All recombination events mediated by FLP or Cre are reversible. Whereas the excision...individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However, if ...recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled... inducible Cre versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible...

  7. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ...cannot help you pass. In addition, activation experiments often require quite a bit of tuning before you...wide variety of activators you can use for your experiments. We have found that SAM (Konermann et al., 2014...be true given the results of previous RNA-seq experiments (Konermann et al., 2014, Chavez et al., 2014,...works reasonably well.  Best of luck on your experiments!  Many thanks to our guest bloggers Marcelle...

  8. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ...Researchers have designed a variety of photoswitchable elements into many other types of proteins. Here, we’ll...sword Microtubules are one of the most important components of the cytoskeleton. They provide cells with ... will surely be useful for a wide variety of experiments. Opto-RhoGEFs manipulate cell morphology and ...phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and 100 times... and cytotoxicity better than the 50x and 100x variants. But those super-high efficiency constructs are...

  9. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ...For such a therapy to be successful, multiple requirements must be met. First, CRISPR would need to be ...desirable. Should stem cell editing occur, the CRISPR components would only need to be expressed for a short period...skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon skipping strategies for...the technology is advancing, precision editing treatments may also be closer than we might expect.   References...expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger...

  10. The 12 Days of CRISPR

    Type
    Blog Post
    Published
    Jan. 3, 2023, 2:15 p.m.
    ...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...prime editor with enhanced editing efficiency in plants 🎵 ...
Showing: 421 - 430 of 842 results