We narrowed to 855 results for: PLE;

1. Cancer, Inflammation and Immunity - Harnessing the Body’s Defenses to Fight Cancer

   Type

   Blog Post

   Published

   July 6, 2017, 1:59 p.m.

   ...state by inducing immunosuppressive measures, for example the upregulation of the immunosuppressive cell ...

2. Hot Plasmids - June 2019 - Optogenetics, Acoustic Reporter Genes, microRNAs, and the CRISPR-Cas9 system CHIME

   Type

   Blog Post

   Published

   June 6, 2019, 12:41 p.m.

   ...ever like to write about a recent plasmid deposit please sign up here.      Listen to this episode of the...

3. Dos & Don'ts When Publishing a Scientific Manuscript

   Type

   Blog Post

   Published

   May 22, 2014, 4:02 p.m.

   ...peer review that occurs post-publication and is completely open; the reviewer’s name and affiliations, and...

4. Antibodies 101: Affinity Reagents

   Type

   Blog Post

   Published

   April 14, 2022, 1:15 p.m.

   ...camelid antibodies to antibody fragments to tools completely unrelated to antibodies.  Why use an alternative...

5. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

   Type

   Blog Post

   Published

   Nov. 3, 2020, 2:15 p.m.

   ...electric current of ion channels in living cells. Multiple properties of a channelrhodopsin, such as cell...

6. Teaching an Old DOG New Tricks: Controlling Protein Activity with GFP

   Type

   Blog Post

   Published

   Nov. 24, 2015, 3:30 p.m.

   ..., GFP linked the two GBPs together to create a complete transcription factor, activating luciferase transcription...

7. Making CRISPR Plasmids Using Fragmid

   Type

   Blog Post

   Published

   Aug. 29, 2024, 1:15 p.m.

   ...(GG) cloning approach. Individual modules and a sample ligation are depicted. (Bottom) Schematic depicting...

8. A Look Back at One Year of Plasmid Sharing for COVID-19 Research

   Type

   Blog Post

   Published

   July 12, 2021, 1:15 p.m.

   ...-LAMP) and a colorimetric readout that can be completed in just 45 minutes. The team deposited the reverse...

9. No Llamas Required - Synthetic Nanobodies Against Membrane Proteins

   Type

   Blog Post

   Published

   June 18, 2020, 1:15 p.m.

   ...the end of the mRNA molecule, forming a ribosome complex consisting of the translated protein (sybody), ...

10. Lentiviral Vector Uses and Overview

    Type

    Blog Post

    Published

    May 19, 2016, 2:30 p.m.

    ...Lentiviral barcoding libraries have been used for complex applications like lineage tracing in heterogeneous...

11. Tips for Screening with Yeast Two Hybrid Systems

    Type

    Blog Post

    Published

    Oct. 22, 2015, 2:30 p.m.

    ...plasmid pEZY202  from Yu-Zhu Zhang’s lab) is an example of the former. In this system, histidine-auxotrophic...

12. Your Lentiviral Plasmid FAQs Answered

    Type

    Blog Post

    Published

    April 23, 2014, 1:08 p.m.

    ...Third-generation plasmids can be packaged with either system. Please note that the generation used to package the virus...

13. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...characteristics that make them more useful than SpCas9. For example, Cas9 from Staphylococcus aureus (SaCas9) is about...

14. Sonic Hedgehog? Sleeping Beauty? Learn About the Genes Behind Addgene’s Conference Room Names

    Type

    Blog Post

    Published

    April 2, 2019, 1:44 p.m.

    ... happens to an organism when a gene/protein is depleted in a knockout experiment. Certain scientific communities...

15. Hot Plasmids: Spring 2025

    Type

    Blog Post

    Published

    June 3, 2025, 1:15 p.m.

    ...  Multiple Authors...