We narrowed to 855 results for: NTS

...structure you want to achieve before starting your experiments. Don’t dive directly into the pipetting! MoClo...be used directly in your assemblies. Preparing reagents Keep it cool You’ve done all the learning and ...What really matters is the molar ratio of your fragments. To increase your ligation efficiency, we recommend...Calculation for the plasmid volume for MoClo experiments. The term ‘insert’ refers to plasmids carrying... but they also work with very small volumes of reagents which lowers experimental costs. If you are planning...

...’s convenient for researchers who want to do experiments in complex eukaryotic cells, but would prefer...resistance gene, so it isn’t a good fit for all experiments. And the list of antibiotic options doesn’t end...Auxotrophic selection is especially common in yeast experiments because yeast tend to spontaneously develop resistance... but it isn’t common for simple transfection experiments. Reporters Finally, in some cases a researcher...gene they simply can’t turn on! Wait, what even counts as a shuttle vector? Are viral vectors shuttle ...

...academic job market? In addition to all of the documents you submit, you will need to present a "chalk .... Given this large sum, one might expect that applicants would work for months to hone a chalk talk. Yet... use the information. In contrast, a list of experiments or a laundry list of molecules to screen has ...is a significant need for healthy livers for transplants. Growth of new livers in culture could resolve...learned and/or now be possible to do? Research accomplishments are remembered for the problem solved, not ...

...produce lower amounts of protein, which can be useful if the product is toxic in high amounts. These plasmids...appropriate time—for example, the cell must have enough nutrients available to complete the entire round of replication... are efficient for the host, as no additional components are necessary. Iterons are not the only tool ...sequence forming this pseudoknot, so its binding prevents the mRNA from folding and blocks RepZ translation...

...recombination and avoid separation of the multiplexed elements. The team designed sgRNAs that include an internal...by this tool as well as additional potential improvements to the editor. Find PE7 plasmids here!   ...in the rhodopsin domain conferred several new improvements. The new variant has increased signal-to-noise...voltage indicator Ace2N-mNeon also led to similar improvements in performance.      Figure 4: Structure...trans-splicing. By splitting a large gene into smaller fragments, delivering them by individual AAVs, and then ...

...origin of replication (ori) contains structural elements that are versatile and adaptable. Variation in...bacterial hosts (Doran et al. 1998). Regulatory elements within the ori also control copy number and stability...differentiated from one another. Based on their structural elements, oris interact with various RNA polymerases and...Host range depends on whether the host has the components to recognize the ori and allow for replication...consist of a plasmid’s ori and all of its control elements, from broad host range plasmids can be used to...

...Cre-lox or one of the FLP-FRT systems in your experiments. Mutated FRT and loxP sites in Flex Vectors –... – inversion only once All recombination events mediated by FLP or Cre are reversible. Whereas the excision...individual neurons one could reduce the plasmids amounts to reduce transfection efficiency. However, if ...recombinase expression while proteins needed for later experiments (cellular tracking for instance) can be controlled... inducible Cre versions can be used in these experiments. This elegant combination of Cre, FLP, and inducible...

...upregulation of essentially any gene by fusing mutants of Cas9 deficient in DNA cutting activity to a ...cannot help you pass. In addition, activation experiments often require quite a bit of tuning before you...wide variety of activators you can use for your experiments. We have found that SAM (Konermann et al., 2014...be true given the results of previous RNA-seq experiments (Konermann et al., 2014, Chavez et al., 2014,...works reasonably well.  Best of luck on your experiments!  Many thanks to our guest bloggers Marcelle...

...Researchers have designed a variety of photoswitchable elements into many other types of proteins. Here, we’ll...sword Microtubules are one of the most important components of the cytoskeleton. They provide cells with ... will surely be useful for a wide variety of experiments. Opto-RhoGEFs manipulate cell morphology and ...phospholipase D (PLD). Now they present a set of variants called SuperPLDs with 10, 30, 50, and 100 times... and cytotoxicity better than the 50x and 100x variants. But those super-high efficiency constructs are...

...For such a therapy to be successful, multiple requirements must be met. First, CRISPR would need to be ...desirable. Should stem cell editing occur, the CRISPR components would only need to be expressed for a short period...skipping therapies would be applicable to 80% of DMD patients. Figure 1: Exon skipping strategies for...the technology is advancing, precision editing treatments may also be closer than we might expect.   References...expression in cells from Duchenne muscular dystrophy patients through genomic excision of exon 51 by zinc finger...