We narrowed to 842 results for: NTS
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TypeBlog PostPublishedMay 23, 2023, 1:15 p.m....consider strategies using other types of affinity reagents like fluorescently-labeled nanobodies or scFvs... label such as horseradish peroxidase (HRP). Experiments using enzymatic labeling can be difficult to ...t forget the controls! The necessary control experiments will vary depending on your specific experimental...design. Like selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk...intensity patterns are similar between the two experiments. If not, it might be that the other antibodies...
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Newly Updated AAV Data Hub!
TypeBlog PostPublishedFeb. 7, 2023, 2:15 p.m....community submissions for Addgene and CLOVER AAV experiments - and we've updated our submission process so... are selecting AAVs that will work for their experiments, and a key step for that is sharing the data ... negative data helps others to design better experiments (and wouldn't it be nice if those weeks and months... the Data Hub to help select an AAV for your experiments, let us know ([email protected]) and we might... -
Plasmid Grant to Aid Cervical Cancer Screening in Ghana
TypeBlog PostPublishedNov. 8, 2016, 3:30 p.m....PCR-based cervical cancer screens. This third grant represents the continuation of our efforts to provide resources...Bengyella and Kwabena Duedu who were awarded similar grants back in June, Gladys will be using plasmids in ...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...Superhero 5k Find New Cancer Resources Learn About Our Grants to Rare Disease Research Resources on Addgene.... -
FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution
TypeBlog PostPublishedOct. 17, 2019, 12:28 p.m....-stimulate mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics...infect defined neuronal subpopulations, and this represents a major downside in optogenetics (Belzung et ... delivery of Cre recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed ...and Peter Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991): 489...neurons: tools, applications, and experimental constraints." Neuron 95.3 (2017): 504-529. PubMed PMID: 28772120... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....understand how to make new treatments and technologies accessible to patients. And what better place to... through engagement with your local or state governments. Most communities have opportunities to join ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...policy fellowships in federal, state, and local governments, professional societies, industry, and beyond... -
Grad School Advice Part 2: Building Community
TypeBlog PostPublishedOct. 6, 2016, 2:30 p.m.... and what makes it difficult for many graduate students. As part of this discussion, we mention graphs...school. This is, of course, not real data, but many students discuss the third year dip (see graph). Niroshi...about picking a post-doc project 19:55 - 22:40 : Improvements that can be made to the grad school process ...'s favorite advice to give to younger graduate students Check Out Angela Depace's Talk on "Precision... -
Your Top Requested Plasmid in 2015!
TypeBlog PostPublishedDec. 30, 2015, 3:30 p.m....key to its efficient use in screening experiments; large amounts of virus are required to ensure that ...plasmid in 2015. In line with all of the exciting developments surrounding CRISPR genome engineering in the... We thank our many depositors for making these reagents available to the research community through Addgene... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....together. Second, KTRs are easy to design. The key determinants of KTR function are the positions of the phosphorylatable...measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between the nucleus...another variable to consider when designing KTR experiments. Third, KTRs are not well suited for monitoring... Regot, S., & Covert, M.W. (2018). Live-cell measurements of kinase activity in single cells using translocation... -
CRISPR-Cas14: a family of small DNA-targeting enzymes enabling high-fidelity SNP genotyping
TypeBlog PostPublishedNov. 29, 2018, 1:53 p.m....are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the... provide bacteria protection against infectious agents. Several enzymes behind this immunity have already...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient... -
Developing Transferable Skills During Science Training
TypeBlog PostPublishedJune 3, 2014, 2:14 p.m....able to talk and own these accomplishments. Employers and academic departments want to be able to tell that...Leadership and management Take a lead from the amazing students at Washington University who started the BALSA...like FierceBiotech to “learn the business” Attend events with local networking and trade organizations ...