We narrowed to 855 results for: NTS

...CRISPR, Addgene gave to me: Functional Genetic Variants Revealed by Massively Parallel Precise Genome ...prime editing systems by manipulating cellular determinants of editing outcomes🎵 On the 5th day of CRISPR...CRISPR, Addgene gave to me: High-fidelity Cas13 variants for targeted RNA degradation with minimal collateral...prime editor with enhanced editing efficiency in plants 🎵 ...

...consider strategies using other types of affinity reagents like fluorescently-labeled nanobodies or scFvs... label such as horseradish peroxidase (HRP). Experiments using enzymatic labeling can be difficult to ...t forget the controls! The necessary control experiments will vary depending on your specific experimental...design. Like selecting an antibody, some of the requirements are similar to standard IF. Here, we’ll walk...intensity patterns are similar between the two experiments. If not, it might be that the other antibodies...

...community submissions for Addgene and CLOVER AAV experiments - and we've updated our submission process so... are selecting AAVs that will work for their experiments, and a key step for that is sharing the data ... negative data helps others to design better experiments (and wouldn't it be nice if those weeks and months... the Data Hub to help select an AAV for your experiments, let us know ([email protected]) and we might...

...PCR-based cervical cancer screens. This third grant represents the continuation of our efforts to provide resources...Bengyella and Kwabena Duedu who were awarded similar grants back in June, Gladys will be using plasmids in ...Seeding Labs Plasmid Grant to a third round of applicants at the start of 2017 and would like to thank ...Superhero 5k Find New Cancer Resources Learn About Our Grants to Rare Disease Research Resources on Addgene....

...understand how to make new treatments and technologies accessible to patients. And what better place to... through engagement with your local or state governments. Most communities have opportunities to join ...boards, support ballot initiatives, deliver public comments, meet with local representatives, or even run ...policy fellowships in federal, state, and local governments, professional societies, industry, and beyond...

...-stimulate mammalian neurons and alter synaptic events: a new technology was born, and the term “optogenetics...infect defined neuronal subpopulations, and this represents a major downside in optogenetics (Belzung et ... delivery of Cre recombinase in mouse primary afferents." Scientific reports 8.1 (2018): 7321. PubMed ...and Peter Hegemann. "Rhodopsin-regulated calcium currents in Chlamydomonas." Nature 351.6326 (1991): 489...neurons: tools, applications, and experimental constraints." Neuron 95.3 (2017): 504-529. PubMed PMID: 28772120...

... and what makes it difficult for many graduate students. As part of this discussion, we mention graphs...school. This is, of course, not real data, but many students discuss the third year dip (see graph). Niroshi...about picking a post-doc project 19:55 - 22:40 : Improvements that can be made to the grad school process ...'s favorite advice to give to younger graduate students   Check Out Angela Depace's Talk on "Precision...

...key to its efficient use in screening experiments; large amounts of virus are required to ensure that ...plasmid in 2015. In line with all of the exciting developments surrounding CRISPR genome engineering in the... We thank our many depositors for making these reagents available to the research community through Addgene...

...together. Second, KTRs are easy to design. The key determinants of KTR function are the positions of the phosphorylatable...measure kinase activity in specific subcellular compartments. Second, because KTRs shuttle between the nucleus...another variable to consider when designing KTR experiments. Third, KTRs are not well suited for monitoring... Regot, S., & Covert, M.W. (2018). Live-cell measurements of kinase activity in single cells using translocation...

...are 24 variants of the cas14 gene that cluster into 3 subgroups (cas14a-c). All these variants share the... provide bacteria protection against infectious agents. Several enzymes behind this immunity have already...many others are still unknown. Identifying Cas14 variants In this quest to discover new and maybe more efficient...