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We narrowed to 842 results for: NTS

Showing: 441 - 450 of 842 results

  1. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...used these knockout mice to show exactly which components of the known pathway that were essential for ... rapidly performed by targeting all potential components of a signaling pathway. For example, one could...PD-L1 on a cancer cell). In this screen, known components of the cytokine signaling pathway could be targeted...target surface proteins to determine which contact points are involved in a studied cellular behavior. This...accelerated immensely when we share knowledge and reagents. I have not intentionally excluded any libraries...

  2. Science as Art

    Type
    Blog Post
    Published
    March 2, 2015, 3:13 p.m.
    ...following the trend and encouraging departments to showcase the work of students and staff by holding scientific...method to explore the inner structures of delicate plants and other organisms without destroying the original...organisms on your lab bench? Let us know in the comments.    Additional Resources on the Addgene Blog ...

  3. Scientific Sharing in the Time of COVID-19: Databases and Resources

    Type
    Blog Post
    Published
    May 12, 2020, 1:15 p.m.
    ... is a database that provides information about reagents, funding and volunteer opportunities, protocols... hopefully help guide policies and ultimately treatments in a timely fashion.”  With this in mind, Bourque...including datasets and platforms for discussing new developments, and more. As researchers around the world continue...community work together to share open protocols, reagents, and other initiatives for #COVID19 research. ...

  4. Year of the Camelid: Antibody Style

    Type
    Blog Post
    Published
    May 9, 2024, 1:15 p.m.
    ...forms of antibodies and had begun developing Fab fragments and scFvs. But the potential for an even smaller... they compare to other antibodies and affinity reagents. But for a quick refresher, some of the advantages...target concave epitopes Suitability for in vivo experiments, due to their simple structure    sdAbs in research... camelid mice and llamas neutralize SARS-CoV-2 variants. Nature 595:278–282. https://doi.org/10.1038/s41586...

  5. Zebrafish as a Model for Behavior: Swimming into the Optogenetic Spotlight

    Type
    Blog Post
    Published
    July 16, 2019, 12:59 p.m.
    ...peak absorption wavelengths and light intensity requirements of different opsins, studies have shown that...generates periodic motor commands for rhythmic movements like spontaneous swimming. To do so, the lab expressed... behaviors, the researchers measured the tail movements of 5 day old fish. The larvae were immobilized... groups of neurons that elicited similar tail movements, they were able to narrow down the possible specific...

  6. Early Career Researcher Toolbox: Free Tools for Making Scientific Graphics

    Type
    Blog Post
    Published
    June 11, 2019, 1:16 p.m.
    ...and hide individual vectors (shapes defined by 2D points connected by lines and curves) that you’ve drawn...Layers are useful for drawing figures with lots of elements. It took some playing around to figure out all...images to the Biorender workspace. Free Biorender accounts can store 5 workspaces at a time and workspaces...resource not mentioned? Tell us about it in the comments section!  Additional resources on the Addgene...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ... in vertebrates. The system is comprised of 2 components: 1) an SB transposase, the enzyme required for... and modifying various extinct transposases. Improvements have been made since then by increasing the ...Zsuzsanna Izsvak’s lab screened many SB tranposase variants for hyperactivity in mammalian cells. Their efforts...inducible expression vectors were developed into 16 variants with different selection markers (blasticidin,...

  8. Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio

    Type
    Blog Post
    Published
    Jan. 15, 2015, 1:50 p.m.
    ...Bozeman Sunday night, it was easy to spot meeting participants. They were the ones holding poster tubes (or...Zhang's introduction. His willingness to share reagents so freely with the academic community has clearly...CRISPR delivery systems, modifications to the RNA components, and challenging target genomes, such as the ...who contact us and want a guarantee that their experiments will work (yes, this happens): Science is hard...

  9. Plasmids 101: Antibiotic Resistance Genes

    Type
    Blog Post
    Published
    Jan. 30, 2014, 3:29 p.m.
    ...antibiotics? Antibiotics are generally defined as agents that kill bacteria, or inhibit their growth. Although...be a bit of a gray area as some bacteriostatic reagents can kill bacteria when used at high concentrations... the mutation. When designing these types of experiments it is best practice not to use the same resistance...Blue White Screening to Simplify Your Cloning Experiments See Where You Can Find Antibiotic Resistance ...

  10. Using Addgene's New Viral Service for Your Research

    Type
    Blog Post
    Published
    Nov. 17, 2016, 3:30 p.m.
    ...if we can help them share and distribute other reagents. Virus distribution came up time and time again...this frustration and getting researchers to the experiments they’re interested in faster by producing, titering...you out. Of course, lentiviruses are used in experiments other than just those involving CRISPR and so...first two can serve as controls in a variety of experiments, while rtTA can be used to activate expression...
Showing: 441 - 450 of 842 results