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...recommends using standard BsaI in place of the high fidelity version (despite what the original paper says)...

...mouse, human or plants,” explained Jan Lohmann of Heidelberg University. “What is different is the plasmids...

...cryptochrome 2 are the chromophores flavin-adenine dinucleotide and methyltetrahydrofolate, which are universally...

...microscopes, amplified staining methods, and oligonucleotide-based staining. Of course, if you’re interested...

...available from Addgene!) Since the signal level provided by SunTag is so high, cells may be imaged under...

... Cryptic loxP sites in mammalian genomes: genome-wide distribution and relevance for the efficiency of...

...as new sdAbs become easier to generate and more widely adopted. So thank you to the camelids who inspired...

...transposon can now bind a strand of DNA with a TA dinucleotide (there are many such sites in the human genome...

... ZFNs through TALENs and CRISPR-Cas nucleases, provided important context for the rest of the day. He ...

...seen the emergence of several SARS-CoV-2 variants coincide with increased vaccination efforts. So, how effective...

...took a dividing yeast and did a genetic screen to identify the factors that regulate cell division. And that...

...Addgene's viral service Find viral protocols and videos Find AAVs for your research ...

...research.     To see Tetbow in action, watch the video below depicting dendrite wiring of mitral cells....

... as a seizure disorder.” In 2012, researchers identified the gene responsible for the disease as ATP1A3...

...equilibrium with ambient air is restored. We have no evidence to suggest that the transient change in pH affects...

...week) so they can easily recall the encounter. As a side note, my guess is that people will not be exchanging...

...reporter that would fluoresce: FlipCherry. FlipCherry provides a proof of concept that the designed used in FlipGFP...

...antigen, pre-existing conditions, etc. that can vary widely between recipients.   In vitro prediction of drug...

...stable FP for your next microscopy experiment, consider hfYFP and LSSmGFP.     Fig. 4: hfYFP retains...

...in optimizing TCSs. Over 75,000 TCSs have been identified in bacteria, and with similar optimization methods...