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We narrowed to 814 results for: LAS

Showing: 461 - 470 of 814 results

  1. Nanoblades: Tiny CRISPR Ninjas for Genome Editing Difficult Cells

    Type
    Blog Post
    Published
    Sept. 26, 2019, 12:50 p.m.
    ...infiltrate cells. Options for CRISPR delivery include plasmid transfection, RNP electroporation, and viral transduction...types they tested. Interested in using Nanoblades? Plasmids are available from Addgene. Genome editing primary...to DNA transfection of gRNA and Cas9 expression plasmids. These results are in line with previous research...sometimes requires intracellular injections into the cytoplasm or pronucleus of the zygote which is both technically...out our comprehensive CRISPR guide Find CRISPR plasmids and resources Learn about viral production ...

  2. Mouse Modeling, Part 1: Genetically Engineered Mice

    Type
    Blog Post
    Published
    July 11, 2019, 1:26 p.m.
    ...in research can fall under one of two broad classifications: inbred or outbred. Inbred strains share a ...embryonic stem cells that are then injected into a blastocyst. There are several types of genetically engineered...Modified embryonic stem cells are injected into a blastocyst which is then implanted into a female animal ...embryonic stem cells, which are then injected into a blastocyst. Nucleases can also be used to engineer knockout...create mosaic animals where not all cells in the blastocyst were successfully edited by the injection of ...

  3. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ... in the laboratory became frustrated with using glass cloning rings to isolate colonies of antibiotic-...growing cells we sometimes use TPP peelaway T-75 flasks to provide a greater surface area (the amount of...contamination. We do not normally use antibiotics. Lastly, and perhaps obviously, it is important to have...Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...

  4. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...antibiotic selectable markers by the destination plasmid. Antibiotic selection allowed for high throughput...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...'s work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find... Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology...

  5. xCas9: Engineering a CRISPR Variant with PAM Flexibility

    Type
    Blog Post
    Published
    March 28, 2018, 6:52 p.m.
    ...transcription from the accessory plasmid (AP) encoding phage gene III. The accessory plasmid library contained all...PAM flexibility. In this system, the mutagenesis plasmid (MP6) permits quick, large-scale mutation of the...doi: 10.1038/nature26155 PubMed PMID: 29512652 Plasmids from this paper are available at Addgene.  Additional...Addgene.org Check out our CRISPR Guide Find CRISPR Plasmids for Mammalian Systems Learn about CRISPR History...

  6. Antibodies 101: Flow Compensation

    Type
    Blog Post
    Published
    May 16, 2024, 1:15 p.m.
    ...you the laser configuration. In general, if two fluorescent markers are read by the same laser, they will... markers you intend to use and your cytometer’s laser configuration. Your flow core or an online manual...reads out the fluorophores FITC and PE on the same laser, you will need to compensate between the two of ...your flow cytometer reads them out on different lasers, you should not need to compensate between them...

  7. Viral Vectors 101: Voltage Indicators

    Type
    Blog Post
    Published
    Jan. 30, 2024, 2:15 p.m.
    ...JEDI, and many more. They can be targeted to the plasma membrane to measure membrane potential and can ...containing GEVIs. Chemigenetic sensors are better classified by their fluorescent reporter and the mechanism...fused to a single fluorescent protein (FP). This class of GEVI was the earliest developed voltage sensor...these sensors contained a FP that localized to the plasma membrane due to the addition of several targeting...lipophilic compound that distributes itself within the plasma membrane as well. Another flavor of these tools...

  8. Which Fluorescent Protein Should I Use?

    Type
    Blog Post
    Published
    May 20, 2014, 2:06 p.m.
    ...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission... relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other FPs have been...emission. For example, if your microscope has only two lasers, at 488nm and 561nm, you will not be able to use... comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review...

  9. Capturing a Moment with Marc Zimmer’s “The State of Science”

    Type
    Blog Post
    Published
    July 30, 2020, 1:15 p.m.
    ...outlets have addressed CRISPR frequently over the last several years, but lay readers may be less familiar...is being done has changed significantly over the last decade. He describes the detection of gravitational...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...competitiveness of U.S. science and technology.” This last section of the book, Future Science, describes the...research. Even if Zimmer was able to rewrite the last chapter of his book today--the day you are reading...

  10. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...smaller vectors suitable for transient assays using protoplast transformation. Cytosine base editors for plant...
Showing: 461 - 470 of 814 results