... in the laboratory became frustrated with using glass cloning rings to isolate colonies of antibiotic-...growing cells we sometimes use TPP peelaway T-75 flasks to provide a greater surface area (the amount of...contamination. We do not normally use antibiotics. Lastly, and perhaps obviously, it is important to have...Time CRISPR User) The Challenges of Cell Culture Plasmids 101: Mammalian Vectors Resources on Addgene.org...Our Protocols Pages Browse Fluorescent Protein Plasmids Find Mammalian Expression Vectors ...

...cells. FPs classified by the emission color (emission wavelength range) FPs are usually classified by emission... relevant mutations, check out Marcy's post from last week on GFP. Additionally, many other FPs have been...emission. For example, if your microscope has only two lasers, at 488nm and 561nm, you will not be able to use... comprehensive data sheets on many FP variants Plasmids 101: Green Fluorescent Protein (GFP) Three Review...

...outlets have addressed CRISPR frequently over the last several years, but lay readers may be less familiar...is being done has changed significantly over the last decade. He describes the detection of gravitational...and cheap distribution of the newest CRISPR-Cas plasmids, has been a key driver of the technology.” As ...competitiveness of U.S. science and technology.” This last section of the book, Future Science, describes the...research. Even if Zimmer was able to rewrite the last chapter of his book today--the day you are reading...

...cells; however, Mycoplasma species do not possess cell walls. As a result, Mycoplasma are intrinsically...resistance are resistant to three or more different classes, or types, of drugs used to treat them. Very often...fantastic example of intrinsic resistance would be Mycoplasma species’ resistance to beta-lactam antibiotics...intrinsically resistant to this class of antibiotic. Acquired resistance can be the result of one bacterium sharing...

...repair mediated by uracil glycosylase (Figure 1). The presence of a uracil glycosylase inhibitor (UGI) minimizes...all CRISPR plasmids and resources at Addgene Find CRISPR base editors Find CRISPR plasmids for plants ...smaller vectors suitable for transient assays using protoplast transformation. Cytosine base editors for plant...

... either nuclear GFP, membrane CFP, cytoplasmic YFP, or cytoplasmic RFP in a specific cell type. The Confetti...et al., 2011) labels Drosophila neurons at the neuroblast stage, meaning that neurons of the same lineage...a specific tissue. XFPs are introduced using a plasmid construct called Zebrabow-GateDest containing an...introduced in vivo either by electroporation of plasmids, or using AAV vectors. The Imai lab also developed...

...Browse fluorescent protein plasmids and resources See all Jaffrey lab plasmids ... cellular processes by transforming cells with plasmids encoding aptamer-tagged RNA and later introducing...aptamer downstream of a known promoter. Addgene’s Plasmids 101: Aptamer Fluorophores describes the enrichment...photostability of the Corn-DFHO complex. For dessert (last but not least), the only fruit-named aptamer, Mango...

...for future preclinical and clinical work. Find Plasmids for Disease Research on Our Special Collections...finger nucleases to remove exon 51 in DMD patient myoblasts. They observed a 13% removal rate of exon 51, ...gRNAs to delete exon 51 or exons 45-55 in patient myoblasts; when injected into DMD mice, these cells expressed... to ascertain how long the rescue phenotype will last, and more importantly, if it does extend lifespan... Check out Our CRISPR Guide Pages Find CRISPR Plasmids for Your Research Find Validated gRNAs for Your...

...research? Check out Brainbow plasmids available from Addgene! Find Brainbow Plasmids @Addgene: Brainbow 1.0...1.0, 1.1, 2.1 plasmids Brainbow 3.0, 3.1, 3.2 plasmids References: Transgenic strategies for combinatorial... of Cre-lox. Check out our previous blog post, Plasmids 101: Cre-lox, if you need a quick primer on how...

...recombinant antibodies (rAbs), which are produced from plasmids and have very little batch-to-batch variation.... of sequence can be done from relatively cheap plasmid sequencing. While rAbs are monoclonal, recombinant... buffer than proteins easily available in the cytoplasm or whole-cell lysate. A harsher, SDS-containing...However, these systems are expensive, generate plastic waste, and limit the amount of optimization that...temperature for 1–2 years and are available in formulas specific to your choice of detection methods. ...antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes and fluorescent...