Skip to main content
Addgene

Enter a sequence to perform a BLAST-based search of the Addgene plasmid sequence database.

Information

In Category

We narrowed to 842 results for: NTS

Showing: 461 - 470 of 842 results

  1. Viral Vectors 101: Viral Applications

    Type
    Blog Post
    Published
    April 13, 2023, 1:15 p.m.
    ... how can you use these amazing tools in your experiments? Let’s unpack the myriad number of emerging viral...more! Libraries allow you to test thousands of elements simultaneously in your system instead of just ... virus to which immunity is desired. Instead, components of the virus are now delivered in other forms...features make them an ideal delivery system for human patients. Multiple viral-based gene therapies have already...

  2. Hot Plasmids - May 2022

    Type
    Blog Post
    Published
    May 26, 2022, 1:15 p.m.
    ... transgenic lines that can be used for other experiments. And, while the BLITZ system was designed and...continuation of that work, they have found at least two variants that display increased neuronal expression in ...side effects, by allowing the user to select for variants that infect the CNS while selecting against those...while potentially allowing for up to 20-plex experiments with one visual barcode per cell and up to 200...

  3. Addgene’s Viral Service - Why Virus? Why Now?

    Type
    Blog Post
    Published
    March 23, 2017, 2:30 p.m.
    ... and Lentiviral preps. This new Viral Service represents Addgene’s largest new initiative since we started...self-supporting service so that Addgene is not dependent on grants. Aiming to operate under a similar model for our...in 2018, our Product Team) continues to make improvements to our website and inventory management systems... were waiting. I look forward to sharing new developments on this project and on all the interesting things...

  4. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...activity is crucial for successful CRISPR editing experiments, there was a real need in the scientific community... in 2017 to enrich and identify desired CRISPR mutants (Wen et al., 2018). SRIRACCHA uses the Piggybac...activity and sgRNA efficiency as well as enrich for mutants through the presence of a fluorescent reporter.... best sgRNA to ensure success in your CRISPR experiments. But how? There have been several systems to ...

  5. Behind the scenes: Addgene’s new search engine and more

    Type
    Blog Post
    Published
    Dec. 12, 2019, 2:22 p.m.
    ...80,000+ reagents and 700+ educational resources — including blog posts, protocols, guides, and collections...Addgenies involved). The new homepage and navigational elements help users who prefer to browse and discover items...implementation, we laid the foundations for many improvements to come. Ultimately, we would like our search... will give our new search engine a try to find reagents and educational material. Let us know what works...

  6. Clear and Accessible Writing

    Type
    Blog Post
    Published
    Feb. 15, 2024, 2:15 p.m.
    ...wait!  Done? Excellent! We asked our workshop participants to do the same thing, and even though only about... equal to the buoyant force of the solution. Contaminants of lower densities are captured at the 17%, ...syringe."Feel free to share your alt text in the comments! We learned a lot from listening to others' alt... Five Steps to Writing Grant Proposals to Grad Students Writing Scientific Manuscripts Tips for Improving...

  7. Plasmids 101: Blue-white Screening

    Type
    Blog Post
    Published
    June 4, 2015, 1:03 p.m.
    ...to a lacZΔM15-mutant bacterial cell in trans complements the mutation allowing for a functional enzyme...when expressed, is lethal to the cell. Cloning fragments are inserted into an MCS in the center of this... susceptible to or dependent on certain media components, and are rescued by genes supplied on the transformed...Expression Systems Find Empty Backbones for Your Experiments    ...

  8. How to Make Friends and Meet People at a Scientific Conference

    Type
    Blog Post
    Published
    Aug. 7, 2014, 1:58 p.m.
    ...these people you meet will review your papers or grants in the future, and don’t be surprised to know that...personally Scientists in your field will know about grants and projects that might help your work Tenure can... people. At large conferences, look for smaller events or meet-ups to connect with others more easily....conference Make sure you have a business card – even students and postdocs. There are many inexpensive/free ...

  9. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...reviewer’s comments without explaining why. If it looks like you have ignored their comments, they will...plasmids (like your Addgene plasmid #), and other reagents. F1000Research, among many other journals, has...researchers have enough information to replicate your experiments, making research more reproducible and your results...

  10. Optogenetics + CRISPR, Using Light to Control Genome Editing

    Type
    Blog Post
    Published
    Sept. 3, 2020, 12:15 p.m.
    ...Scientists around the world have been making major improvements to CRISPR technology since its initial applications..., heterodimerizing domain to each of the Cas9 fragments, the authors created a photoactive Cas9 tool, ...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...in turn transcribes N-Cas9. The N- and C-Cas9 fragments then dimerize to form a functional protein due...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...
Showing: 461 - 470 of 842 results