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Addgene

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We narrowed to 842 results for: NTS

Showing: 471 - 480 of 842 results

  1. Plasmid Tools for Microbiome Studies

    Type
    Blog Post
    Published
    June 27, 2019, 12:25 p.m.
    ...microbiome to deliver a genetic payload to the recipients using the IncP⍺-family-RP4 conjugation system...they only identified seven genera in in vitro experiments, possibly because in vitro conditions did not...The vector retains the oriT for delivery into recipients by conjugation. The labs screened for the host-range...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...

  2. Antibodies 101: Selecting the Right Antibody

    Type
    Blog Post
    Published
    March 15, 2022, 1:15 p.m.
    ...and planned out your key experiments. Unsurprisingly, many of these experiments require antibodies. You...antibody may be validated for IHC, not all IHC experiments are the same. Differences in sample processing...antibody could meet your needs.    Multiplex experiments Finally, consider whether you will be using other...

  3. Antibodies 101: Secondary Antibodies

    Type
    Blog Post
    Published
    Dec. 21, 2021, 2:15 p.m.
    ...approach or indirect approach, depending on the requirements of the specific assay. For the latter three,...indicated when the protein of interest is found in low amounts or if the conjugated signaling molecule has a weak...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...primary antibodies. The introduction of antibody fragments, such as nanobodies, has introduced new options...

  4. Addgene: The First Twenty Years

    Type
    Blog Post
    Published
    Dec. 19, 2024, 2:15 p.m.
    ...really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical...process by aligning institutions with standardized agreements and electronic signatures to dramatically speed...nominated for it, it just reflects how useful the reagents we created are for the community. More than 100...were useful for researchers wanting high quality reagents, and we realized we could use the same principles...

  5. All in a Twist: dsRNA

    Type
    Blog Post
    Published
    Feb. 2, 2023, 2:15 p.m.
    ...of dsRNA Transposable elements such as long/short interspersed nuclear elements can generate dsRNA by ...bidirectional transcription or as inverted repeats. These elements are almost always epigenetically silenced, but... are important considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that...

  6. Addgene's Tips for Plasmid Quality Control

    Type
    Blog Post
    Published
    Jan. 14, 2016, 3:30 p.m.
    ...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ... Each sequencing service will have different requirements for volume and concentration, and not all providers..., you can use DNA-editing software to perform alignments of multiple sequences to visually check for homology...Tell us your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene...

  7. Working with Nuclear Receptors

    Type
    Blog Post
    Published
    July 15, 2014, 4:03 p.m.
    ...datasets and reagents in the field. Additional information can be found in the Datasets and Reagents sections... third phase of funding, the NURSA Consortium represents a unique funding model in nuclear receptor research...and acquiring them in a timely manner. Addgene represents an excellent solution to the problem of sharing...

  8. Synthetic promoter AAVs for cell-type specific expression in retinal cells

    Type
    Blog Post
    Published
    March 12, 2020, 1:15 p.m.
    ...labeling of neurons for tracing experiments.  Neuronal tracing experiments: While synthetic promoters with...the retina, it was more likely to have borrowed elements from a natural promoter that was active in the...labeling would be useful for neuronal tracing experiments where lower levels of labeling makes it easier...

  9. Hot Plasmids May 2018 - Optogenetics, Decaffeination, Biosensors, and Fluorescent Protein Tools

    Type
    Blog Post
    Published
    May 15, 2018, 12:43 p.m.
    ...clusters without accumulation in subcellular compartments such as nuclear speckles. Furthermore, CRY2clust... distinguish real and artifactual postsynaptic currents (PSCs) this work shows that the artifactual PSCs...TagRFP-T fluorescence intensity at different time points, the authors tracked protein activity while also... assays. ShadowG is darker than other dark YFP mutants, it reduces the risk of spectral contamination ...

  10. Plasmids 101: The Wide World of Natural Plasmids

    Type
    Blog Post
    Published
    Oct. 8, 2024, 1:15 p.m.
    ... survive in competitive environments — like by living where no one else wants to. Some organisms manage...their host organisms to thrive in competitive environments. The genes encoding these functions form the...cryptic plasmid is among the most numerous genetic elements in the human gut. Cell, 187(5), 1206-1222.e16....
Showing: 471 - 480 of 842 results