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We narrowed to 842 results for: NTS

Showing: 481 - 490 of 842 results

  1. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ..., or universities may have someone senior from accounts or HR on the judging panel. It’s important for...Skills 5. Practice makes perfect, apply for small grants too There are actually quite a lot of different... researchers, so look into applying for smaller grants from within your institute or a private funding...the experience I gained in writing small travel grants to attend conferences and a seed funding grant ...

  2. Tips for Technical Support Calls

    Type
    Blog Post
    Published
    Sept. 20, 2016, 2:39 p.m.
    ...companies and organizations selling biological reagents have a ton of data on the catalog page. It’s not.... Although many may not realize it, all of the reagents available at Addgene come from researchers, and...don’t have the resources to recapitulate the experiments performed by our thousands of depositors. That...Google Scholar, which searches scientific articles, patents, and even some doctoral dissertations. Streamline...

  3. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ... it can amplify DNA using only a short list of reagents and several heating and cooling steps. PCR relies...strand of DNA to add nucleotides to.  Using these reagents and a series of heating (denaturing) and cooling...cycle. However, it is common for dNTPs and other reagents to run low during the final cycles which would...of different molecular biology techniques and experiments. End point PCR’s goal is to amplify a specific...

  4. Adenoviral Delivery of CRISPR/Cas9 Aims to Expand Genome Editing to Primary Cells

    Type
    Blog Post
    Published
    Dec. 10, 2020, 1 p.m.
    ... corrective amounts of protein for any such disease. This technology therefore represents a potential ...In vivo engineering of oncogenic chromosomal rearrangements with the CRISPR/Cas9 system. Nature 516:423...doi.org/10.1038/srep05105 Stephens CJ, Lauron EJ, Kashentseva E, Lu ZH, Yokoyama WM, Curiel DT (2019) Long-...

  5. The Scientific Conference Poster Session: Tips for Success

    Type
    Blog Post
    Published
    Aug. 29, 2019, 12:50 p.m.
    ...microbiology meeting. Create an outline: Write key bullet points for what you would like to say to help build your...result, and the fundamental takeaway. Writing these points will help you remember what to say and provide ...outline you created for yourself. Hit the key bullet points and elaborate if your audience seems captivated... the project as a whole. In addition to time constraints, there may be particular poster you are interested...

  6. Academic vs. Industry Postdocs

    Type
    Blog Post
    Published
    Nov. 13, 2018, 1:22 p.m.
    ...to develop therapies that could directly impact patients. And, to be honest, I’ve never enjoyed grant-writing... process can be a bit of a mystery to graduate students in academia. Getting a drug to the market usually...pages. In academia, however, writing papers and grants is an inescapable and essential part of being able...’re often required to at least attempt to write grants. If you don’t enjoy or excel at writing, an industry...

  7. PCR Applications for Copy Number Variation Assays

    Type
    Blog Post
    Published
    April 26, 2022, 1:15 p.m.
    ...number of cycles.  Choosing a method  As with all experiments, a number of factors will determine which approach...Resources needed Most molecular biology labs and departments will have qPCR machines available for use. ddPCR...Chung WK. Quantitative analysis of copy number variants based on real-time LightCycler PCR. Curr Protoc... The copy-number of plasmids and other genetic elements can be determined by SYBR-Green-based quantitative...

  8. Production of Virus in Insect Versus Mammalian Cells

    Type
    Blog Post
    Published
    Nov. 5, 2024, 8:32 p.m.
    ...produced in multiple different cell types, including plants, mammals, and insects. In this post, we will compare...producing virus within 24 to 48 hours. Harvesting timepoints will depend on your specific virus, so this is...methods like ultracentrifugation, while lentivirus supernatants are centrifuged and filtered to eliminate any... important to you — virus type, cost, time, or reagents and equipment. Hopefully this post has provided...

  9. Viral Vectors 101: Producing Your rAAV

    Type
    Blog Post
    Published
    July 16, 2024, 1:15 p.m.
    ...post-transfection to help remove toxic transfection reagents and keep the cells happy. Harvesting virus Once...widely used method due to the accessibility of instruments in standard labs. When determining titers by ...Adeno-Associated Viral Vectors by Oxidant-Based Disinfectants. Human Gene Therapy, 32(13–14), Article 13–14...sensitivity sufficient to detect single infectious events. Human Gene Therapy, 15(7), Article 7. https://...

  10. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...the degron domain is terminal. Between the tag elements (protease and degron) and the protein of interest...other systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir...reviewed here. The AID system relies on three components. First, the protein of interest must be AID-tagged...SMASh). If you don’t want to deal with additional components beyond the minimum required for a degron system...
Showing: 481 - 490 of 842 results