We narrowed to 855 results for: NTS

...reviewer’s comments without explaining why. If it looks like you have ignored their comments, they will...plasmids (like your Addgene plasmid #), and other reagents. F1000Research, among many other journals, has...researchers have enough information to replicate your experiments, making research more reproducible and your results...

...Scientists around the world have been making major improvements to CRISPR technology since its initial applications..., heterodimerizing domain to each of the Cas9 fragments, the authors created a photoactive Cas9 tool, ...photoactivatable repressor (dCas9). The activity of all variants was reversible and repeatable. Since these initial...in turn transcribes N-Cas9. The N- and C-Cas9 fragments then dimerize to form a functional protein due...Engineering of temperature- and light-switchable Cas9 variants. Nucleic Acids Research 44: . https://doi.org/...

...really appreciate the work you do to make sharing reagents and protocols so easy!” — Nina Jain, Harvard Medical...process by aligning institutions with standardized agreements and electronic signatures to dramatically speed...nominated for it, it just reflects how useful the reagents we created are for the community. More than 100...were useful for researchers wanting high quality reagents, and we realized we could use the same principles...

...of dsRNA Transposable elements such as long/short interspersed nuclear elements can generate dsRNA by ...bidirectional transcription or as inverted repeats. These elements are almost always epigenetically silenced, but... are important considerations when using these reagents. RNA:DNA hybrids Persistent RNA:DNA hybrids that...

...and planned out your key experiments. Unsurprisingly, many of these experiments require antibodies. You...antibody may be validated for IHC, not all IHC experiments are the same. Differences in sample processing...antibody could meet your needs.    Multiplex experiments Finally, consider whether you will be using other...

...approach or indirect approach, depending on the requirements of the specific assay. For the latter three,...indicated when the protein of interest is found in low amounts or if the conjugated signaling molecule has a weak...interest is often one of the most challenging components of any immunoimaging assay. Secondary antibodies...primary antibodies. The introduction of antibody fragments, such as nanobodies, has introduced new options...

...microbiome to deliver a genetic payload to the recipients using the IncP⍺-family-RP4 conjugation system...they only identified seven genera in in vitro experiments, possibly because in vitro conditions did not...The vector retains the oriT for delivery into recipients by conjugation. The labs screened for the host-range...without fixation or preparation, and deliver CRISPR components for gene disruption or CRISPRi. While designed...

...growth instructions (temperature, strain, media supplements), suggested sequencing primers, or mammalian ... Each sequencing service will have different requirements for volume and concentration, and not all providers..., you can use DNA-editing software to perform alignments of multiple sequences to visually check for homology...Tell us your favorite sequencing tricks in the comments section below.  Additional Resources on the Addgene...

...datasets and reagents in the field. Additional information can be found in the Datasets and Reagents sections... third phase of funding, the NURSA Consortium represents a unique funding model in nuclear receptor research...and acquiring them in a timely manner. Addgene represents an excellent solution to the problem of sharing...

... survive in competitive environments — like by living where no one else wants to. Some organisms manage...their host organisms to thrive in competitive environments. The genes encoding these functions form the...cryptic plasmid is among the most numerous genetic elements in the human gut. Cell, 187(5), 1206-1222.e16....