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We narrowed to 599 results for: REV;

Showing: 41 - 50 of 599 results

  1. Prime Editing: Adding Precision and Flexibility to CRISPR Editing

    Type
    Blog Post
    Published
    Jan. 13, 2025, 9:49 p.m.
    ...them! PE2: Improving reverse transcriptase efficiency Building upon prior reverse transcriptase research...humans and cataloged in the ClinVar database. Previously developed genome editing methods using nucleases...prime editor consists of a Cas9 nickase fused to a reverse transcriptase. The Cas9 nickase is guided to the...sequence hybridizes to the target site, and the reverse transcriptase domain copies the desired edit, directly...wild-type Moloney Murine Leukemia Virus (M-MLV) reverse transcriptase to the C-terminus of Cas9 H840A nickase...every ~8 base pairs on either DNA strand, many previously developed base editors with a <8 base-pair editing...the edit, the Liu lab turned to a strategy they previously used when they developed base editing. By nicking...

  2. Plasmids 101: Degron Tags

    Type
    Blog Post
    Published
    March 14, 2024, 1:15 p.m.
    ...systems reviewed here. The only required components are 1) a SMASh-tagged protein and 2) asunaprevir (small...and exogenously expressed proteins. Here we will review what advantages degron tags can offer over other...systems – CRISPR, siRNA, etc. Firstly, they are reversible mechanisms of control. Protein degradation can...the drug has been removed. The degradation and reversal are rapid compared to other systems because the...comes to degrons, you have options! Below we will review the 4 most common types of degrons and some of ...the presence of a specific protease inhibitor, asunaprevir, the viral protease can no longer self-cleave...addition to having the coolest name of all the tags reviewed here, the SMASh tag does offer a few other unique...

  3. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...formal peer review. Some organizations, including some journals, also support open peer review or curation...journal-based peer review can be prone to biases or errors, with a lack of transparency in most review processes...authors on a public server independent from peer-reviewed publishing in a journal.  Accelerating scientific...scientists and advocates have argued that while peer review is valuable, modern scientific publishing has a...or endorsements. In contrast, journal-based peer review is usually a confidential process among a few individuals...productivity that can be shared with hiring and review committees, as opposed to the opaque (and, admittedly...preprints, and a growing number of funding bodies and review committees encourage and consider preprints as ...

  4. Plasmids 101: Yeast Vectors

    Type
    Blog Post
    Published
    Feb. 25, 2014, 7:11 p.m.
    ...L-hisitidine no S. cerevisiae  no yes   URA3 pyrimidine (uracil) yes - Grow with 5-FOA. S. cerevisiae yes - This...of a nitrogen source. S. cerevisiae  no no   LEU2 L-leucine no S. cerevisiae yes - This can complement...explain how they differ from the bacterial elements previously described. Why do scientists use yeast vectors...pertains to ORIs in budding yeast, Saccharomyces cerevisiae; however, we’ve also noted some features required...Schizosaccharomyces pombe, vectors at the end.) In a previous post, we briefly discussed how the regulation ...if specific regulatable elements are included (reviewed here). Plasmids for use in S. pombe, on the other... TRP1 L-tryptophan yes - Grow with 5-FAA. S. cerevisiae no  no TRP1 alters some yeast phenotypes. This...

  5. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...publication of peer reviews so we can see how and why papers change throughout the review process. This will...reproducibility. Cause 1: Inability to repeat previously performed experiments Researchers should be able...incredibly useful technique simply by following the abbreviated methods section found within a published manuscript...additionally averse to producing papers that repeat previously published results. This over-reliance on the ...will hopefully push reviewers towards more productive criticisms that focus less on perceived novelty ...recognizing that we’re biased and taking steps to prevent our biases and goals from effecting our experiments...things I’ve read comes to this: we need others to review our experimental designs and we need to be transparent...

  6. K. phaffii: Rising to the Occasion

    Type
    Blog Post
    Published
    April 4, 2023, 1 p.m.
    ...and it grows fast to boot. The yeast strains S. cerevisiae and S. pombe have dominated the research scene...are there even other yeasts? In this blog we will review an alternative model yeast strain and discuss why...used yeast strains: S. pombe (fission) and S. cerevisiae (budding). Yeast make for an excellent model ...genetic manipulation The first eukaryote (S. cerevisiae) to have its genome fully sequenced There are...wondering if the “biotech yeast” is S. pombe or S. cerevisiae. Actually, it’s neither. The most commonly used...phaffii and other yeast strains.     S. cerevisiae S. pombe K. phaffii Growth properties... is only distantly related to S. pombe and S. cerevisiae; evolutionarily it evolved much slower and bears...

  7. Plasmids 101: Repressible Promoters

    Type
    Blog Post
    Published
    Dec. 20, 2022, 2:15 p.m.
    ...only active under specific circumstances. In a previous post, we discussed inducible promoters, which ...bind to the operator. The bound repressor then prevents transcription from occurring, which means that...to the tetracycline operator sequences (TetO), preventing transcription. In the presence of tetracycline...binds tTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...as well as the inducible manner detailed in our previous post. In the past 30 years since the original ...binds cTA, resulting in a confirmation change that prevents binding to the promoter and turning transcription...as well as the inducible manner detailed in our previous post. While originally developed for use in mammalian...

  8. Hot Plasmids Spring 2024

    Type
    Blog Post
    Published
    May 23, 2024, 1:15 p.m.
    .../10.1101/2023.12.12.571321.  Revolutionizing Gene Therapy: The REVeRT Dual AAV Vector System By Vaibhav...sensors AAV vectors for targeting microglia The REVeRT Dual AAV Vector System Neurodegeneration Collection..., 2019, including modifications to the Cas9 or reverse transcriptase enzymes, inhibition of host cell ...traditional AAVs, the Becirovic Lab has developed REVeRT, for gene delivery using reconstitution via mRNA...2023 under a CC-BY license.  The authors used REVeRT to functionally reconstituting coding sequences...disease-relevant large proteins. They demonstrated REVeRT in various contexts in vitro and in vivo, including...human retinal organoids and live mice. Importantly, REVeRT avoids the introduction of extraneous genetic material...

  9. Multiplex Genome Editing with CRISPR-Cpf1

    Type
    Blog Post
    Published
    May 9, 2017, 2:12 p.m.
    ...-depth review of Cpf1, check out this blog post or see Addgene's CRISPR guide page for a review of Cas9... cleave the lentiviral RNA, preventing packaging of the virus.  Reversing the orientation of the direct...RNA from Cpf1-mediated cleavage. This reversing would also prevent processing of the crRNA array when expressed...generally more efficient. Pro-Tip Make sure to reverse the orientation of the crRNA’s direct repeats when...expressed in cells, but notice how Zetsche et al reverses the orientation of the promoter driving expression...(bolded red arrow).  This undoes the original reversing of the direct repeats, leading to expression of...

  10. Advice on Career Paths and the Green Card Process for International Researchers and Entrepreneurs

    Type
    Blog Post
    Published
    Dec. 20, 2017, 2:33 p.m.
    ...Performing peer review at the request of your supervisor is not considered peer review. The peer review request...achievements. Participating in the scientific review process as a reviewer as proof of your judging the work of...already reviewing papers that you should start contacting journal editors to request to be a reviewer. Proactivity... Participating as a judge (including as a peer reviewer for journals, acting as a journal editor) of the...you specifically. You must also complete the peer review for it to be counted as proof of judging. Merely...case.  You can still assist your PI in the peer review process, but remember you cannot use it as documentary...evidence for your case – the invitation to be a peer reviewer needs to be addressed to your own personal email...
Showing: 41 - 50 of 599 results