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We narrowed to 79 results for: plasmid cloning by pcr

Showing: 41 - 50 of 79 results

  1. Plasmids 101: Multicistronic Vectors

    Type
    Blog Post
    Published
    Sept. 9, 2014, 8:20 p.m.
    ...with a plasmid that already has the multicistronic element and reporter cloned in. In these plasmids you ...more Plasmids 101 posts or browse Addgene's Plasmid Guide for more molecular biology and cloning information...you would simply clone your gene of interest into the multiple cloning site up or down stream of the IRES...unique genes, you can start with a plasmid that has multiple cloning sites flanking the multicistronic ...interest. Some of the plasmids listed in the table above (and their related plasmids) are designed to have...replaced.  Additionally, 2A peptides could be PCR-cloned between your genes of interest and you can then... a one-stop reference guide for plasmids. We've combined our Plasmids 101 blog posts and additional resources...

  2. Ghanaian Researchers to Receive Plasmid Awards from Addgene and Seeding Labs

    Type
    Blog Post
    Published
    June 15, 2016, 1:30 p.m.
    ...construction of new plasmids. Dr. Duedu will also use polymerases produced using plasmids from Addgene to ...pathogens in environmental samples via PCR. We hope these plasmids will help Dr. Duedu inspire new Ghanaian... has been working with Seeding Labs to provide plasmid grants to researchers in developing countries. ... to announce that we’ve selected our first two plasmid grant awardees: Drs Louis Bengyella and Kwabena...doctors Bengyella and Duedu and how they plan to use plasmids from Addgene to advance their research. Louis ...molecular biology work has focused on using various cloning methods and tools to construct chimeric cellulases...cellulosic biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular...

  3. Addgene's Top 10 Blog Posts of 2015

    Type
    Blog Post
    Published
    Dec. 7, 2015, 6 p.m.
    ... 4. Plasmids 101: Blue-White Screening Getting a lot of empty vector contaminants in your cloning experiments...experiments and tired of having to PCR many colonies to find a successful clone? Addgenie Jessica Welch explains...CRISPR mediated genome engineering. 10. Plasmids 101: Control Plasmids Need help thinking about the types ...Scientists eBook Download the Plasmids 101 3rd Ed. eBook Read the Most Recent Plasmids 101, CRISPR 101, and Career... two eBooks (Management for Scientists and the Plasmids 101 2nd ed, the 3rd edition has now been released...top of the many developments in science and the plasmids, technologies, and researchers behind them. As...most popular posts continue to be those from our Plasmids 101 and CRISPR 101 series. These posts aim to ...

  4. Multiple Plasmids at a Low Price: Kits from Addgene

    Type
    Blog Post
    Published
    Sept. 14, 2016, 2:30 p.m.
    ...can order from the repository: FX Cloning Kit BIOFAB Plasmid Set pCri System Cell Free Protein Expression...curated collections of plasmids as kits with greatly reduced costs per plasmid. We generally create kits...for cloning into the final translated products. Negative selection markers built into all plasmids in ...just the tool you need. The plasmids in this portion of the BIOFAB Plasmid Set contain terminators of ...interest. The plasmids used to generate these fusions all use the same restriction enzymes for cloning. Furthermore...populations. This kit consists of a 48 plasmid adapter library, with each plasmid carrying a unique barcoded Illumina-M13F...researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. ...

  5. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... are cloned into Golden Gate entry vectors via traditional restriction enzyme cloning, TA cloning, or ... Hot Plasmid list. Other popular plasmids & blog posts @Addgene: The 10 Most Distributed Plasmid Technologies...inducible inactivation. Find the Robinson plasmids or the Royle plasmids and get to work! Robinson et al...Assemble multiple component plasmids from building blocks via Golden GATEway Cloning Frustrated by the complexity...constructs? A new cloning system combining the advantages of Golden Gate and Multisite Gateway cloning methods ...that you need. The Golden GATEway cloning kit simplifies the cloning process for complex DNA constructs...final transgenesis construct. Plasmids constructed using Golden GATEway cloning have been utilized to create...

  6. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...we’d create a plasmid similar to the one shown below, where the sequence of GFP is cloned together with...DNA designed in the lab. Specially constructed plasmids can be used to replace genes in yeast, mice, or...gene. Here, we'll describe the techniques and the plasmids used to inactivate specific genes in mammalian...mind when choosing a knockout strategy! Knockout plasmids Homologous recombination is a mechanism to accurately...to exons 1 and 3 of the targeted gene have been cloned into the vector on either side of an antibiotic...randomly integrated all or large portions of the plasmid. Deliver your construct to your target cells. After...Cre-mediated recombination.   Knockout/Knock-in plasmids Gene targeting methods also make it possible to...

  7. Adenoviral Vector Production and Troubleshooting

    Type
    Blog Post
    Published
    June 14, 2018, 11:44 a.m.
    ... consists of 2 plasmids: a shuttle vector (in which the transgene of interest is cloned) and pAdEasy™ ...lines such as HEK293 cells. 1st generation AdV cloning capacity is limited to 8.2 kb, and in vivo transgene.... 2nd Gen AdVs were designed to have increased cloning capacity, decreased  potential for the generation...deleted in 2nd generation AdVs, thereby increasing cloning capacity to ~12 Kb. The ever popular pAdEasy system... interest for gene therapy due their increased cloning capacity (up to 36 kb!), long-term transgene expression...down into 5 key steps: Construction of the rAdV plasmid (~1 week): The AdEasyTM system is the most popular...how to generate the final recombinant adenoviral plasmid construct, please see Addgene’s Adenoviral Guide...

  8. Golden Gate Assembly Upgrades: More Fragments, Faster Assembly, and Higher Fidelity

    Type
    Blog Post
    Published
    Oct. 11, 2018, 12:30 p.m.
    ...by the destination plasmid. Antibiotic selection allowed for high throughput cloning efficiency measurements...work on ligase efficiency Learn about other plasmid cloning techniques Resources on Addgene.org Find ...Find basic molecular biology protocols Find plasmid cloning protocols Check out our molecular biology reference...More complex 24-fragment assembly Single insert cloning efficiencies were measured based on the acquisition... selection. Screening of transformants by colony PCR confirmed the insertion of the Lambda insert at the...accurate assemblies was achieved by the sequencing of plasmids isolated from blue or white colonies. Sequencing...occasional uncut or cut/re-ligated pGGA destination plasmids. A final validation of the 5-, 12- or 24-fragment...

  9. Interview: Nicola Patron on Plant Synthetic Biology, MoClo, and More

    Type
    Blog Post
    Published
    June 12, 2014, 3:30 p.m.
    ... are in the lab. There is no PCR, no gel purification; the whole cloning process is so simplistic once...Addgene: Tell me about the Golden Gate Modular Cloning (MoClo) Kit. What does it include? Patron: Sainsbury...them. The MoClo Plant Kit makes it really easy to clone genes for expression in plant cells. Sylvester and...already use. It makes the switch to Golden Gate Cloning easier because individual labs will not need to...make it easier for people to use the Golden Gate cloning system especially with plants in mind. When it’... 30 constructs. They’ve never done any kind of cloning and they don’t have any prejudice about it. They... biology.  References: A Golden Gate Modular Cloning Toolbox for Plants. Carola Engler, Mark Youles,...

  10. CRISPR 101: Multiplex Expression of gRNAs

    Type
    Blog Post
    Published
    Sept. 10, 2020, 11:45 a.m.
    ... rectangles) are cloned into various plasmids using oligonucleotides. These plasmids contain Type IIS ... use mammalian plasmids to introduce you to some of your potential options and cloning methods, but please...multiple gRNAs on the same plasmid, you’ll make sure that each cell that gets the plasmid contains all of the... this plasmid, you simply order oligonucleotides for your chosen gRNA target sequences and clone them ...Gibson Assembly or SLIC cloning methods. A BsaI-based E. coli multiplexing plasmid is available from the...different purposes.   Gersbach Lab multiplexing plasmids: This plasmid set allows you to express 2-4 gRNAs, with... ever need to clone in filler sequences. To build your multiplexing construct, you clone all but one of...
Showing: 41 - 50 of 79 results