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...Addgene blog Plasmids 101: What is a plasmid? Plasmids 101: Blue-white screeningPlasmids 101: Common lab...

....   Expressing shRNAs using plasmids The design of shRNA plasmids requires three important steps: ...shRNA plasmids are being designed using RNA polymerase II promoters. The design of these plasmids is meant...with Cas13a (C2c2) Plasmids 101: The Promoter Region – Let's Go! Browse All Plasmids 101 Posts Additional...Following delivery into cells using viral vectors or plasmids, shRNAs hijack the endogenous miRNA machinery ...by a complex called Drosha to pre-miRNA. miRNA plasmids are engineered to skip this first pri-miRNA step...don’t need additional flanking sequences, shRNA plasmids are a bit smaller, with the full sequence being...60 base pairs. This is much shorter than miRNA plasmids, which require additional flanking regions comprised...

... for More Plasmids 101 Articles?  Find them all here: http://blog.addgene.org/topic/plasmids-101   ...In a previous Plasmids 101 blog, we reviewed the salient features of several popular strains of E. coli...strain? Protein expression from high-copy number plasmids and powerful promoters will greatly exceed that...BLR (DE3) Tetracycline RecA-deficient; best for plasmids with repetative sequences.  F- ompT lon hsdSB... strains is not recommended as these auxillary plasmids may be isolated during the prepping process. How...expression work? As mentioned above, many expression plasmids utilize inducible promoters, which are 'inactive...replication, making it incompatible with other p15A plasmids. pLys comes in two flavors—pLysS and pLysE—the...

...Vectors Looking for More Plasmids 101 Articles?  Find them all at our Plasmids 101 Topic Page!     ...synthesis Ptrc-ccdA   Propagation of ccdB-containing plasmids Hte   High transformation efficiency deoR ...for deoxyribose synthesis Allows uptake of large plasmids hee   High electroporation efficiency supE44...Survival 2 T1R   TOP10 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway...Streptomycin  HB101 derivative. For propagating plasmids expressing the ccdB gene (important in Gateway...DH5alpha   General cloning and storage of common plasmids, blue/white screening. F- endA1 glnV44 thi-1 recA1...Cloning large inserts and transformation of large plasmids. F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74...

...Heidelberg University. “What is different is the plasmids you use to bring this into your target organism...

...underrepresented plasmids (plasmids that were lost or “dropped out”) and overrepresented plasmids (plasmids that ...library contains many plasmids, care must be taken to ensure that all of these plasmids are amplified and ...diversity. If the various plasmids in the library are differently sized, smaller plasmids may be amplified preferentially...In addition to single plasmids, Addgene also distributes pooled plasmid libraries containing hundreds...hundreds, thousands, or even a million plasmids. These libraries are some of Addgene’s most exciting and versatile...growth of other cells. For libraries where the plasmids are all very similar sizes, like a gRNA library...2. You can use a small PCR product because the plasmids in the library are identical except for the insert...

...Addgene “Find Plasmids” badge in the image below. If an abstract on PubMed has the “Find Plasmids” badge, you...you’ll find new plasmids. Simply click on the badge to be taken directly to the plasmids on Addgene’s site...you should see the “Find Plasmids” box on papers that have deposited plasmids. Now that you know how ...Order Plasmids from Addgene Check Out Our FAQ Pages Browse Plasmids ...thought - Is there an easy way to find published plasmids? Do you spend a lot of time in PubMed searching...you can use PubMed to see which articles contain plasmids deposited at Addgene from the abstract page. Check...article to ensure you understand the features of the plasmids, how they were created, and how to use them in...

... of LIC is often limited to specially designed plasmids. Sequence- and ligation-independent cloning: A...

...plasmid DNA. Among those that do, some now have plasmids that contain your gene of interest, but others...kanamycin-containing agar. Only cells carrying plasmids that contain a kanamycin resistance cassette will... for cloning are auxotrophic for specific amino acids. When plated on media lacking the essential amino... created a yeast prototrophy kit containing 23 plasmids to complement auxotrophy. Negative selection: ... property by including ccdB on its destination plasmids. In the destination plasmid, the ccdB gene is ...Additional resources on the Addgene blog Read our Plasmids 101 blog posts Learn more about Lambda Red recombineering...counter-selection Resources on Addgene.org Find plasmids at Addgene Read our molecular biology reference...

... Gulp! Though bacteria don't exactly "eat" plasmids as depicted in this comic, when a bacterium uptakes...’s genome. To do this scientists commonly use phagemids, a DNA cloning vector that contains both bacteriophage...bacteriophage and plasmid properties. The phagemids are packaged into replication-incompetent phage particles...find all kinds of different lentiviral and AAV plasmids as well as ready-to-use viral preparations at ...requires the use of a fine needle to deliver nucleic acids to individual cells. Electroporation on the other...resources on the Addgene blog Read all of our Plasmids 101 blog posts Check out all of our blog posts...

...that took up the vector and distinguish between plasmids that contain the gRNA (white) from those that ... it turns into an insoluble blue pigment. Find plasmids for blue-white screening at Addgene! Some bacterial...way to distinguish between colonies containing plasmids with inserts from those that do not is by using... video on using restriction digests to analyze plasmids, which can help you visualize this process, or...Addgene blog Get all the plasmid basics in our Plasmids 101 series Find more molecular biology protocols...

...Addgene Blog Plasmids 101: Common Lab E. coli Strains Plasmids 101: TOPO Cloning Plasmids 101: Golden ...SurvivalTM, one can efficiently propagate and prep plasmids containing the ccdB gene, which can then be used...difficult to transform, depending upon the specific plasmids being used. We would also like to note that while...should never be used when selecting for recombinant plasmids. Check out our blog post on Common Lab E. coli...

...researcher at a nonprofit institution, you can order plasmids covering a wide range of fields from Addgene. ...that Addgene distributes curated collections of plasmids as kits with greatly reduced costs per plasmid...create kits to allow you to easily use a set of plasmids together on a tight budget. To help popularize... production (a variety of pExpression vectors) plasmids. The type IIS restriction sites used in these ...products. Negative selection markers built into all plasmids in the kit help decrease false positives observed...to a variety of experimental opportunities. The plasmids in this kit have been used to purify a variety... BIOFAB and the Endy Lab created this kit. The plasmids within it contain a variety of different promoters...

...hot plasmids in our newsletter or on our hot plasmids webpage. Listen to the entire Hot Plasmids podcast...In the second episode of our Hot Plasmids podcast series, you'll learn about new red fluorescent proteins...podcast! Hot plasmids covered in this segment Hot Plasmid 1: The Tol2 Gateway-Compatible Toolbox for quickly...Receive Our Newsletter Check Out All of Our Hot Plasmids Articles Browse the Repository  ...

...with the Plasmid Name, Addgene Plasmid ID #, and the PubMed ID # (PMID) for the article that has used ...it has been validated in.  If a plasmid's Addgene ID # has been referenced in other publications, you'...your deposited plasmids Increased visibility of your plasmids in the "Associated Plasmids" lists Benefits...Find other related plasmids in the "Associated Plasmids" lists Properly citing plasmids you've requested... from Addgene Plasmids 101: How to Verify Your Plasmid Plasmids 101: Control Plasmids 6 Tips for Analyzing...'ll find the plasmids listed below the citation. An example of these "Associated Plasmids" is shown in...assistance finding plasmids on Addgene's website or would like to contribute plasmids you have made to ...

...collection of plasmids is available in an easily searchable format on Addgene’s Michael Davidson Fluorescent...Michelle Baird, who has created plasmids as a technician in the Davidson lab for the last four years. “...mEmerald plasmids offer a comprehensive list of readily available targets since the Davidson lab always...work of Michael Davidson and his colleagues." Acknowledgments Thank you to Michael Davidson, Michelle Baird...and their use for imaging cell biology, Michael Davidson’s lab at Florida State University has been the ...Molecular Expressions collection including some 3,300 plasmids along with image galleries and educational resources...Fluorescent Protein Collection webpage. These plasmids are divided into the following categories: Over 300 ...

...There are no universal rules for naming plasmids but here are some good guidelines to follow in order...can use in the lab, check out more posts in our Plasmids 101 series.      ...

...information on shipping plasmids to your country. Curious about how rabies plasmids are used in the lab? ...If you're an international researcher who uses plasmids containing genes from rabies and vesicular stomatitis...may have noticed it’s been easier to order these plasmids. This is because in 2024 certain export control...controls prescribed by the Australia Group, for these plasmids for the majority of the countries we ship to, ...lasting basis.   Table 1: Popular Rabies and VSV-G Plasmids Name Purpose Depositing Lab pCMV-VSV-G ...control exceptions for rabies and VSV-G genes, plasmids ship out approximately three weeks faster, as ...required. If you have any questions on ordering plasmids, please feel free to email [email protected]. We...

...some plasmids will not result in one complete, circular assembly. Most of the time, these plasmids are ...Arnold. Introducing NGS in 2017 for all incoming plasmids was a big change for Addgene - we had used Sanger...in-house sequencing! In addition to sequencing plasmids, we have also started using NGS to check the quality...about one day, even for six plates (576 individual plasmids)! These libraries are then QC’d, pooled, and prepared...normalize each to fall within a 10 fold range. Plasmids that don't meet the lower bound of this range ... performing quality control of newly deposited plasmids is to align the NGS result to a reference sequence...Genbank files that are useful for more complicated plasmids, like those that contain synthesized regions that...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles ...'ll make it easier for you to find and use the plasmids you need. If you'd ever like to write about a ... Figure 1: The base editor is split into two plasmids and co-transfected into cells for reconstitution...overcome this overlap, Yiyang Gong’s lab created two plasmids containing a red-shifted Ace-mScarlet, a fusion...channelrhodopsins. Find the Ace-mScarlet voltage indicator plasmids at Addgene! Beck et al., Sci Report 2019. PubMed...fragments. This system has been used to construct plasmids for use in model organisms such as E. coli and...for cyanobacteria. Figure 3: Assembly of plasmids using the Cyanogate Kit and subsequent transformation...