...is busy and has a To-Do list long enough that there is no hope of accomplishing it all. How do we encourage...teams at Addgene will forgo their regular To-Do lists (other than emergency items) and focus on a single... Hack Days, and we’ve seen great success at accomplishing “we-should-really-make-time-for-that” type projects...wonderful tradition for university departments to establish, with the goal of fostering better communication...

...along the way in this ongoing series of blog posts. Listen to our podcast interview with Harvard Medical School...’ – inspire you.” “Also a mentor can give you realistic expectations. (Don't think you can finish that...is instructive (and entertaining) to review this list of bad mentor behaviors. Sadly, it is Yoda who is... concrete goals and helps you reach your goals Listens well and communicates clearly and openly Gives ...like to be validating without being coddling, to listen without being taken advantage of, and to respect...

...CRISPR-based phenotypic screen datasets. Original published CRISPR screen data is curated and deposited in...in BioGRID ORCS to allow facile queries of published data by biomedical researchers and ready access to...identifiers and links provided, in addition to a list of individual screens curated for the publication... approach serves to maintain consistency with published screen interpretations and allows users to query...curators at [email protected] to submit their published data for rapid curation review and upload. In ...

... full picture and listen for hints at the same concerns from multiple people. Listen to what they say,...path and help you pursue any career alternative? Listen to our podcast interview with Harvard Medical School...Ask alumni of the lab. Most labs have websites listing alumi and it is easy to use publication records... end of this blog post. This document includes a list of questions to ask your potential advisors and ...

... a recent plasmid deposit please sign up here. Listen to the Hot Plasmids Podcast!  MarathonRT: A highly...transcriptase Article contributed by Will Arnold  Listen to the MarathonRT podcast segment Researchers from... surface Article contributed by Marcy Patrick  Listen to the nanobody toolkit podcast segment Nanobodies...Caspex  Article contributed by Susanna Bachle  Listen to the Caspex podcast segment Steven Carr’s lab... C-BERST Article contributed by Klaus Wanisch  Listen to the C-BERST podcast segment Similarly to the...

...seems like another dragon to slay on the way to publish your work in a scientific journal. The peer-review...touchstone of the scientific method, ensuring that published work meets the accepted standards of reporting...read, I pay more attention to details and use a checklist for individual sections of the paper (see figures...authors put in the manuscript. As a non-native English speaker, I sometimes miss the sensitivity to judge...sharing whatever she knows with whoever cares to listen. In March 2020 she will start her own research ...

...   Enzyme-linked immunosorbent assay (ELISA) Similarly, ELISA is used to detect the presence of a single...samples. Get more details on ELISAs in our article Antibodies 101: ELISA (Enzyme-linked Immunosorbent ...example, cell lysate or media from cultured cells. ELISA can be used to quantitatively measure the amount...in a series of samples, such as Western blots or ELISAs, or for other experiments to study protein-protein...

...professors how they hire scientists for their labs. Tom Ellis selects new hires from a pool of CVs collected from...collaborations. Alternatively, perhaps the lab publishes and presents regularly with labs around the world...you might enjoy working closely with others to accomplish your goals. You must understand your own style...for Scientists". This post also includes a useful list of questions for a candidate to ask lab heads, lab... awhile. Acknowledgments Thank you to Dr. Tom Ellis (Imperial College London), Dr. Connie Cepko (Harvard...

...a recent plasmid deposit please sign up here.  Listen to the hot plasmids episode! New, highly efficient...knockdown Article contributed by Alyssa Cecchetelli  Listen to the C. elegans RNAi podcast segment RNA interference... miniTurbo Article contributed by Eric Perkins  Listen to the TurboID and miniTurbo podcast segment A ... outbreaks Article contributed by Izzy Mueller  Listen to the viral detection podcast segment As the ... motifs Article contributed by Michael Lemieux  Listen to the SH3 domain podcast segment The human proteome...

...each listed: An “official” name for the container to be used in cross-team communication A list of “AKA...lexicon. The base lexicon was and continues to be a list of commonly used (and misused) words that clearly...the training frequently. In hindsight, getting a list of frequently used words like this when starting...Happier colleagues. Feeling like people aren’t listening to you or understanding you can be incredibly ...

...plasmid deposit please sign up here. No time to read? Listen to our Hot Plasmids Podcast! TRIM-Away: Targeted...degradation Article contributed by Mary Gearing Listen to the TRIM-Away podcast segment CRISPR and RNAi...nanoscopy Article contributed by Eric J. Perkins Listen to Endosomal pH Sensors podcast segment The first...epigenome Article contributed by Joanne Kamens Listen to the Fire-Cas9 Podcast Segment Jerry Crabtree... in yeast Article contributed by Tyler J. Ford Listen to the Synthetic Yeast Transcription Factors Podcast...

...scientists”  These comments are well correlated with published data. For example, in 2010, less than 15% of postdocs...many notable contributions in this area include publishing the book ‘How Economics Shapes Science’ and contributing... Biology at Brandeis University, has recently published an article arguing against reducing the number...postdocs. Among other involvements, he helped to establish grants through the Sloan foundation for postdoc-related...employ them, the diversity of trainee backgrounds, publishing from a young scientist’s perspective, and the...

...helped me remember them for the interview.  Prepare a list of questions to ask your interviewers You’re interviewing...“questions to ask an interviewer,” you can find lists that have lots of general questions about job responsibilities...before the interview. If you are not provided with a list of who will be interviewing you, ask the hiring ... this information. I found it useful to write my list of questions in a notebook that I took with me to.... This is a chance to highlight your prior accomplishments and demonstrate your science communication ...

... set up an ELISA kit company on whose website you can find some great information on ELISA troubleshooting...originality to your work is to collaborate with specialists in different fields that can provide different...insight into an area - not just working within the established network of your peers. Furthermore, using new...of the work was. Ideally this would be results published in a paper, but positive outcomes can include ...

...Biotechnology Communications Specialist at AsisChem Inc. and Social Media Content Specialist at Thermo Fisher Scientific...landscape facing all scientists, both young and established, as they embark on or continue their careers ...effective advocacy strategies on various levels, with panelists including: Kate Stoll, PhD - Senior Policy Advisor... Programs at Personal Genetics Education Project Lisa Coleman, PhD - Chief Diversity Officer Harvard University...

...recent plasmid deposit please sign up here.     Listen to the Hot Plasmids episode! Expanding the optogenetics...CRY2clust Article contributed by Brook Pyhtila  Listen to the CRY2clust podcast segment The Won Do Heo...optogenetics Article contributed by Meghan Rego  Listen to the soCoChR podcast segment Optogenetic stimulation...operon Article contributed by Angela Kaczmarczyk  Listen to the synthetic decaffeination operon podcast ...fluorescent proteins for use as FLIM-FRET acceptors  Listen to the FLIM-FRET acceptor podcast segment Article...

... physical titer are direct p24 measurements via ELISA and qPCR for viral RNA. Measuring p24 levels in ...as there are several commercially available p24 ELISA kits that can quantify p24 levels in a matter of...drawback of this method, however, is that a p24 ELISA measures all p24 in the sample regardless of whether...alternative to direct p24 measurement. Like a p24 ELISA, this method is relatively quick, providing results...than a day, but tends to be less expensive than an ELISA kit. In this approach, viral RNA is first converted...

...This post was contributed by Jae Lee and Pantelis Tsoulfas of the Department of Neurological Surgery ...semiconductor (CMOS) sensors, it takes only a few milliseconds to acquire one image, thereby significantly ...  Thank you to our guest bloggers, Jae Lee and Pantelis Tsoulfas. Jae K. Lee is an assistant Professor...Medicine. Find plasmids from the Lee Lab here. Pantelis Tsoulfas is an Associate Professor at the University....janelia.org/lab/keller-lab http://www.physikalischebiologie.de http://www.lavisionbiotec.com/ultramicroscope-overview.html...

...contribute openness, clarity, approachability, and professionalism to team meetings and projects. It is crucial...identify your transferable skills? PLoS One recently published an article identifying gaps in scientists' transferrable...communication skills quite a bit. Grab a piece of paper and list all the skills that your current or past job requires... get your mind working, take a look at the short list of transferrable skills here and consider which ...Leadership Problem Solving Creativity Data analysis Listening Negotiation Career Planning Resources Cross team...

...path.  A part of the career path story for most established grant professionals is learning to succeed in... with clear budget narratives, logic models, established partnerships and collaborations, and commitments... panels and to review education materials for publishers. When the community orchestra needed a grant ... to enter the grants profession? The four steps listed below can give you the resources and path to help...professional development strategy builds on the steps listed above and formalizes your development. You may ...

...the fact). #9 Thou shalt make a to-do list, and a to-don’t list Prioritize! Some things can wait and some...This article was originally published on the Society for Laboratory Automation and Screening (SLAS) Electronic...tactics to plan your work and make this happen. Be realistic and: Your co-workers will learn to work within...your family, for yourself and for fun to the to-do list. #10 Thou shalt also have fun I’m really busy, but...

...these people at the time, but I met some very accomplished scientists and some rising stars in the world...world of microbiology, some before they had even established their own labs.  Fried chicken with a side of...worth addressing. Clear expectations have been established for future participants regarding the activities...Johnston, Kat Milligan-Myhre, Kim Dill-McFarland, Lisa Gorski, Mark O. Martin, Maureen Berg, Michael D....Apologies to anyone I’ve inadvertently left off this list. Many thanks to our guest blogger Joe James from...

... category by listing off things that fall into that category? Okay, let’s play. I’ll list the examples...examples and you try to guess the category: ELISA...qPCR...Digital droplet PCR...DNA dot blot...Transduction...can be affected by many experimental factors. To list some complicating factors for quantifying AAV: ...Titration of AAV-2 particles via a novel capsid ELISA: packaging of genomes can limit production of recombinant...

...implementation, and now must focus on managing the established partnership. If you’re willing to do similar ... worked with people outside the department to accomplish a variety of goals. For example, I worked with...from other universities and maintained a mailing list. This project gave me practice reaching out to other...some advice and guidance. The relationships you establish in this way can, of course, lead to jobs and even...common interest so naturally this may be easier to accomplish with people at your organization. You can meet...

...sequence assembly of the zebrafish genome was published in 2013, revealing that about 70% of human genes...networks that regulate processes from development to metabolism to fertility. Several zebrafish-specific gene...for generating transgenic zebrafish are well established (Felker and Mosimann, 2016). One common and robust...zygote transforms into an organism with a clearly established body plan including head, eyes, and tail. Compared...different methods have been developed, relying on established reagents such as CRISPR/Cas9, Tol2, and the high-quality...

... encouraged to not just wait for papers to be published, but to engage in ongoing, real-time dissemination...No transformation techniques are available yet. Polistes (Amy Toth) These wasps are like the ones that ...Lab (@EvoEmbryo) September 24, 2018   Xenopus tropicalis (Gary Gorbsky) This is not a new experimental...with RNAi to make a polygamous species monogamous. Anolis sagrei (Doug Menke) No methods were available to...A paper on stickleback fish science was first published in 1867. It said they were hard to raise because...

...not enough. A 2013 study showed that out of 238 published scientific articles, only 46% could be reproduced... have software tools that help us efficiently accomplish our daily goals. These tools remind us of our...In their ideal form, ELNs provide users with a holistic means to manage their work, combining data, team...there will be no sharp changes in costs once they establish the system within their institution. In the study...supportive the ELN team is. Once you’ve made your list of ELNs, create some free or demo accounts and schedule...

...open-access journal that enables high school students to publish peer-reviewed scientific research. You’ll meet ...submitted to the journal with funding from Addgene. Listen to the interview with the Journal of Emerging Investigators...Arooba Ahmed, Jiachen Lee, and Jillian Parker who published their work on a protein required for cytokinesis... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...Plasmids Podcast Listen to our interview with synthetic biology researcher Tom Ellis Resources at Addgene.org... in George Church’s lab as a graduate student. Listen to the interview with Wei Leong Chew! Podcast breakdown... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides 72...first pay attention to the validated applications listed on the vendor’s website and choose an antibody ...applications, pay close attention to the controls listed on the vendor’s website. Typically, the vendor ...method such as an enzyme-linked immunosorbent assay (ELISA) (Aydin et al., 2015). Post-translational modifications...

...Originally published May 3, 2017 and last updated Sep 24, 2020 This post was contributed by guest blogger...Gene knockout with CRISPR technology is usually accomplished by Cas9-mediated dsDNA breaks: following a cut...nevertheless still lead to activity has been established, and can be used to identify sites that are likely... for functional genomics, with an ever-expanding list of tools to probe gene function. The best tools ....1093/nar/gkt555 Resources at the Addgene Blog Listen to our Podcast with John Doench Learn How to Conduct...

...equipment that will help accelerate his research. Listen to learn more about Dr. Kwapata’s current work ...more scientific collaboration across the globe. Listen here! Podcast Breakdown (Time: Topic) 0:00-1:00... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...through Seeding Labs Resources on the Addgene Blog Listen to Additional Addgene Podcasts Learn How You Can...

...now works on protein therapeutics at Novartis - listen to learn more about Crystal’s exciting career path...new projects when old ones prove unsuccessful. Listen to the interview with Crystal Shih Podcast breakdown...applying for industry jobs and deciphering job listings. Mobility between different positions at Novartis... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

... own nonprofit, this is the episode for you.   Listen to the interview with Addgene's founders Podcast...Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5 - Realizing that Addgene...Harvard University, where she studied diabetes and metabolism. Melina has worked at Genetics Institute in Cambridge... Edward B. Page is a Digital Media Production Specialist at Addgene. He is the audio engineer and producer...

...who creates human-animal hybrids. This book is published 30 years after the seminal work of Gregor Mendel...into the twenty amino acids. Once scientists established that DNA encoded all the information necessary...giants such as Isaac Asimov, Arthur C. Clarke, James Blish, and John W. Campbell. This period is termed as ...specific.  Jurassic Park by Michael Crichton was published in 1990, just a year after the discovery of meganucleases...Packer MS, Badran AH, Bryson DI, Liu DR (2018) Publisher Correction: Programmable base editing of A•T to...

...CRISPR use grows across the life sciences and to establish standardized validation techniques for academia...starting point for most researchers as it provides a list of putative off-target sites that they can later...limited to off-target sites of <4 bp. The off-target list is also generally weighted by the position of the...silico prediction to create a more comprehensive list of off-target editing events. Two of the most common.... Outside of the lab, he works as a freelance journalist and science writer with a focus on the ways science...

... with another lab, in another field maybe, and publish Join a lab that has multiple collaborations, even..., resume writing, and networking Practice your English if you need to, go to ESL classes Practice your...technique and be able to talk and own these accomplishments. Employers and academic departments want to... for Researchers called Vitae. Vitae recently published a career development framework for career skills...

...1); similar to an MRI or CT scanner. This is accomplished by focusing excitation light to a point in the... bleaching) to a single focal point. This is accomplished by using, for example, red light instead of ...can a a high enough density of red-photons be establish for this to happen.Using red light has a second... on the sample, a temporal resolution on the millisecond scale may be needed. Most commonly these experiments... be afraid to seek out expertise from a well-established microscopist at your institution when heading...

... my comments: Everyone on the team talks and listens in roughly equal measure, keeping contribution ...regularly seeks expertise wherever it can be found. Listen to our podcast interview with Harvard Medical School...to feedback with your actions     Use active listening skills such as the PAC Method. P = demonstrate...Addgene Blog to follow the entire series as it is published. For more reading try The Discipline of Teams ...

...save a lot of time later. Protein purification specialists recommend doing some research, notably determining...Tags. Beyond E. coli: purifying from Bacillus subtilis and Pichia pastoris pCri also includes plasmids...than E. coli, specifically the bacterium Bacillus subtilis and the yeast Pichia pastoris. The improved secretory.... pCri vector pCri-13a, designed for use in B. subtilis, contains the MISTIC (membrane-integrating sequence...