We narrowed to 488 results for: mme

...likely capabilities of RNA-guided gene drives in the summer of 2014 (after extensive discussions with biosecurity...sake of laboratory convenience. Disclaimer: Recommendations concerning the appropriate degree of confinement...

...to this established dye structure have yielded commercial panels of advanced rhodamine fluorophores, such...Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke D. Lavis...

...to purchase the Cas9 protein and a gRNA from a commercial vendor, this can often be expensive. An alternative...transcribed from ssDNA, which can be generated by commercial vendors such as IDT. These two components are...

...than 50 cycles is recommended. Decrease your ramp rate. A rate of 2C/s is recommended to ensure an even...

...a time.   Not everything that is received is immediately transformed and stored. Some samples need to ...to organize samples within your lab, we highly recommend using barcodes! Barcoded tubes are easily accessible...

... tag for protein purification and a number of commercial antibodies to GFP are available. Others: We’ve...interactions.   Do you have a favorite way to use GFP?  Comment and tell us how! And come back next week when we'll...

...librarians and data curators, IT managers, the commercialization office, the CIO’s office, and research computing...of the vibrant life sciences research and commercialization community in greater Boston, while at the ...

...bevacizumab has recently been added to the treatment recommendations for non-resectable mesothelioma by the National.... Senate. If this bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that...

...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...

...20-nt guide region binds to the DNA sequence immediately downstream of the TAM. This RNA-DNA hybrid formation...in your target gene. Choose a 20-nt sequence immediately downstream of the TAM for targeting. While plant-specific...

... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured...

...we hear the word ‘antibiotic’, very often we immediately imagine a prescription for a bacterial infection...drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & HM Government (2016...

...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

...a single gene they want to activate, we would recommend using a cDNA overexpression vector rather than...closest to the transcription start site (TSS). We recommend targeting the guides to a region less than 200...

... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ...

...pair palindromic repeats separated by an 8 bp asymmetric core. Recombination between these sites can lead...discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is ...

...rest of the genome.  2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM...where the edit is desired, and it performs the programmed edit within the editing window (bases 4-8 of ...

...Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large...

... translation requires the start codon of AUG immediately downstream of a kozak sequence. The Dow lab hypothesized....org/10.1038/mtna.2016.48 Katti A, Foronda M, Zimmerman J, Diaz B, Zafra MP, Goswami S, Dow LE (2020) ...

... mycoplasma contamination. There are several commercially available mycoplasma detection kits that use... example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced ...

...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email.... These should be plainly stated. I would not recommend putting these into slide format. You want people...

...something to brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots...about your favorite new biosensor plasmids in the comments below!   Additional resources on the Addgene ...

...antibodies and refer to the manufacturer’s recommendations.  As you optimize your protocol, always remember...To learn more about antibody validation, this commentary by Uhlen, et al. 2016 is a good place to start...

....org/10.1038/mt.2010.205 Trapani I, Colella P, Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi... Simone S, Colella P, Iodice C, Polishchuk EV, Sommella A, Colecchi L, Rossi S, Simonelli F, Giunti M,...

...countries around the world and to do so, the WHO recommends one strategy: testing, tracking, and social distancing...recombinase polymerase amplification (RPA) using a commercially available amplification kit. In this case, regions...

...into the field. Current versions are already commercially available although they are reported to have...your favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger...

...just natural differences between individuals. Recommended controls for antibody-based experiments Primary...above, viability and compensation controls are recommended for flow cytometry. A viability control allows...

...Marketing Manager at Promega. His work focuses on commercializing their cell and protein analysis products. You... M. P., Butler, B. L., Wood, M. G., Otto, P., Zimmerman, K., Vidugiris, G., Machleidt, T., Robers, M. ...

...predict the perfect titer, but one of the main recommendations when using AAVs is to avoid freezing and thawing... ScAAV avoid this rate limiting step and can immediately undergo replication and transcription. However...

... before you start an experiment with CRISPR The hammer, the jigsaw, and the wrench are all great tools...ignored. Created with BioRender.com. The hammer: Gene knockout by NHEJ Gene knockout with CRISPR...

... Additional libraries are also available from commercial vendors. These libraries usually contain 4-12...community. It shows how science can be accelerated immensely when we share knowledge and reagents. I have not...

...inactivated and less photons are emitted leading to dimmer fluorescence. In fact, many of the early reversible... a favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different...

...distinguish a signal from noise; therefore, it is recommended to use brighter fluorophores for markers that...marker to be highly abundant, it is fine to use a dimmer fluorophore. However, these aren't strict rules...

...residues. While these Cy/NTA conjugates are not commercially available, they are easily synthesized with ...Griffin, Adams, & Tsien, 1998), these dyes are now commercially available as Lumio Green and Lumio Red from ...

... are accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic...optimal time period between viral injection and commencement of behavioral experimentation.  Whether you ...

...information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics of...

...Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides...

...seem to find an antibody that works, we highly recommend “Chapter 3: Finding the Right Antibody for Your...

...scientific communities and welcome suggestions in the comments.  X has moved significantly away from the functionality...

...the seventh day of CRISPR, Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease...

...progress in all areas of research. We welcome your comments... Where do you think these technologies are going...

... The video channels are also underrated and I recommend checking them out, especially since videos on ...

...process takes 4-6 weeks on average. Therefore, we recommend placing your order well in advance whenever possible...

...you don’t see here? Give us suggestions in the comments section below! Additional Resources on the Addgene...

...focus on cloning and plasmid-based technologies. Commentary about scientific sharing and the open-access ...

... for us (If you’re new to plasmids, we highly recommend checking out the video). Tom has made many more...

...know what you think about the calculator in the comments section below. References 1. Choi, Vivian W.,...

...Alex hopes to help Addgene advance - plants for trimmed down protocols, distribution of new materials (...

...Addgene's favorite lunch... a BonMe noodle meal, crammed right into the box. Which is your favorite team...