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We narrowed to 481 results for: mme

Showing: 201 - 250 of 481 results

  1. Better Dyeing Through Chemistry & Small Molecule Fluorophores

    Type
    Blog Post
    Published
    Sept. 8, 2016, 2:30 p.m.
    ...to this established dye structure have yielded commercial panels of advanced rhodamine fluorophores, such...Mensh, a wordsmith soldier of fortune, for helpful comments. Many thanks to our guest blogger, Luke D. Lavis...

  2. Tips from the Repository Trenches: Using Barcodes to Track Samples

    Type
    Blog Post
    Published
    Oct. 6, 2015, 2:30 p.m.
    ...a time.   Not everything that is received is immediately transformed and stored. Some samples need to ...to organize samples within your lab, we highly recommend using barcodes! Barcoded tubes are easily accessible...

  3. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

    Type
    Blog Post
    Published
    May 15, 2014, 3:33 p.m.
    ... tag for protein purification and a number of commercial antibodies to GFP are available. Others: We’ve...interactions.   Do you have a favorite way to use GFP?  Comment and tell us how! And come back next week when we'll...

  5. Supporting Reproducibility with a Connected ELN

    Type
    Blog Post
    Published
    Jan. 17, 2019, 1:38 p.m.
    ...librarians and data curators, IT managers, the commercialization office, the CIO’s office, and research computing...of the vibrant life sciences research and commercialization community in greater Boston, while at the ...

  6. Mesothelioma - Causes, Symptoms, and Treatment

    Type
    Blog Post
    Published
    Dec. 13, 2016, 3:30 p.m.
    ...bevacizumab has recently been added to the treatment recommendations for non-resectable mesothelioma by the National.... Senate. If this bill passes, it will put an immediate stop to the use of asbestos, thus ensuring that...

  7. Antibiotic Resistance: An Old Solution but a New Problem

    Type
    Blog Post
    Published
    Nov. 10, 2016, 3:30 p.m.
    ...we hear the word ‘antibiotic’, very often we immediately imagine a prescription for a bacterial infection...drug-resistant infections globally: final report and recommendations." London: Wellcome Trust & HM Government (2016...

  8. Using Video to Share Your Science: We Share Science

    Type
    Blog Post
    Published
    Nov. 3, 2016, 2:30 p.m.
    ...the study, or even review Facebook and Twitter comments. On We Share science, video usurps the written...the University of Maine, about her research on plummeting embryo survival rates. Video on We Share Science...

  9. Bioinformatics at Addgene

    Type
    Blog Post
    Published
    July 14, 2022, 1:15 p.m.
    ...Numpy, and Biopython.  We started at the usual recommended place: documentation. We oriented ourselves with...process. For example, our bbduk process created trimmed FASTQ files as output, which we specified as input...

  10. New CRISPR Tools: Cas7-11 and PASTE

    Type
    Blog Post
    Published
    Feb. 17, 2022, 2 p.m.
    ...unprecedented specificity: when purified, it could be programmed to generate specific cuts on the RNA target. ...non-dividing and primary cells, and can be easily programmed for new genes. In comparison with other large...

  11. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...under CC-BY 4.0 License. Importantly, even commercial antibodies can be tethered on the viral surface...thick-section and whole-mount labeling techniques. The Trimmer lab’s anti-PSD-95 [K28/43R] recently passed their...

  12. Hot Plasmids: Fall 2024

    Type
    Blog Post
    Published
    Nov. 21, 2024, 1:30 p.m.
    ... variable and rabbit constant domains and is recommended for use in western blots (Figure 1). If you prefer...worm-optimized wrmScarlet. mScarlet-I3, while initially dimmer, showed greater photostability and also matured...

  13. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type
    Blog Post
    Published
    Oct. 13, 2022, 1:45 p.m.
    ...rest of the genome.  2) The target is present immediately upstream of a Protospacer Adjacent Motif (PAM...where the edit is desired, and it performs the programmed edit within the editing window (bases 4-8 of ...

  14. Advanced Uses of Cre-lox and Flp-FRT - A Neuroscientist’s View

    Type
    Blog Post
    Published
    Oct. 19, 2017, 1:54 p.m.
    ...pair palindromic repeats separated by an 8 bp asymmetric core. Recombination between these sites can lead...discovered that it is not the exact sequence of the asymmetric target site core but its 8 bp length that is ...

  15. Plasmids 101: Codon usage bias

    Type
    Blog Post
    Published
    Sept. 27, 2018, 1:09 p.m.
    ... from Addgene now have full sequence data, we recommend directly analyzing gene sequences for codon optimization...thereby increase their abundance. For example, the commercial Rosetta E. coli strains express a variety of ...

  16. CRISPR Activation: A Practical Guide

    Type
    Blog Post
    Published
    Aug. 18, 2016, 2:30 p.m.
    ...a single gene they want to activate, we would recommend using a cDNA overexpression vector rather than...closest to the transcription start site (TSS). We recommend targeting the guides to a region less than 200...

  17. Getting the Most from Your Lentiviral Transduction

    Type
    Blog Post
    Published
    April 7, 2016, 2:30 p.m.
    ... mycoplasma contamination. There are several commercially available mycoplasma detection kits that use... example, while freeze thaw is generally not recommended, Holic et al. found that lentivirus produced ...

  19. SARS-CoV-2/COVID-19 Detection Methods Based on CRISPR/Cas

    Type
    Blog Post
    Published
    May 5, 2020, 1:15 p.m.
    ...countries around the world and to do so, the WHO recommends one strategy: testing, tracking, and social distancing...recombinase polymerase amplification (RPA) using a commercially available amplification kit. In this case, regions...

  20. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...antibodies and refer to the manufacturer’s recommendations.  As you optimize your protocol, always remember...To learn more about antibody validation, this commentary by Uhlen, et al. 2016 is a good place to start...

  21. Tips for Getting a Faculty Position

    Type
    Blog Post
    Published
    July 20, 2023, 1:15 p.m.
    ...improve his eBook, adds: "If you have any questions, comments or suggestions, please do not hesitate to email.... These should be plainly stated. I would not recommend putting these into slide format. You want people...

  22. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...something to brighten our day, whether it’s memories of summer or just a bright new plasmid. One area with lots...about your favorite new biosensor plasmids in the comments below!   Additional resources on the Addgene ...

  23. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...into the field. Current versions are already commercially available although they are reported to have...your favorite "Historical Lab Technique" in the comments section below! Many thanks to our guest blogger...

  24. How to Design Your gRNA for CRISPR Genome Editing

    Type
    Blog Post
    Published
    Sept. 24, 2020, 1:15 p.m.
    ... before you start an experiment with CRISPR The hammer, the jigsaw, and the wrench are all great tools...ignored. Created with BioRender.com. The hammer: Gene knockout by NHEJ Gene knockout with CRISPR...

  25. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ...just natural differences between individuals. Recommended controls for antibody-based experiments Primary...above, viability and compensation controls are recommended for flow cytometry. A viability control allows...

  26. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...predict the perfect titer, but one of the main recommendations when using AAVs is to avoid freezing and thawing... ScAAV avoid this rate limiting step and can immediately undergo replication and transcription. However...

  27. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ... Additional libraries are also available from commercial vendors. These libraries usually contain 4-12...community. It shows how science can be accelerated immensely when we share knowledge and reagents. I have not...

  28. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...inactivated and less photons are emitted leading to dimmer fluorescence. In fact, many of the early reversible... a favorite use for PA-FPs? Let us know in the comments section below! Currently, there are over 20 different...

  29. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...distinguish a signal from noise; therefore, it is recommended to use brighter fluorophores for markers that...marker to be highly abundant, it is fine to use a dimmer fluorophore. However, these aren't strict rules...

  30. Special Delivery: Fluorophore Targeting for FRET Studies

    Type
    Blog Post
    Published
    July 19, 2016, 2:30 p.m.
    ...residues. While these Cy/NTA conjugates are not commercially available, they are easily synthesized with ...Griffin, Adams, & Tsien, 1998), these dyes are now commercially available as Lumio Green and Lumio Red from ...

  31. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ... are accurate and reproducible. I also highly recommend spending the money on a high quality stereotaxic...optimal time period between viral injection and commencement of behavioral experimentation.  Whether you ...

  32. Beginner's Guide to Viral Vectors

    Type
    Blog Post
    Published
    June 2, 2017, 2:30 p.m.
    ...information that's out there, Addgenie Leila Haery has summed up some of the most important characteristics of...

  33. A Case Study of Addgene Bioinformatics

    Type
    Blog Post
    Published
    May 25, 2023, 1:15 p.m.
    ...Last summer, we posted about our bioinformatics quest, which led us to Seqera Labs, a company that provides...

  35. The 12 Days of CRISPR: 2021

    Type
    Blog Post
    Published
    Dec. 28, 2021, 2:15 p.m.
    ...the seventh day of CRISPR, Addgene gave to me: programmed genome editing by a miniature CRISPR-Cas12f nuclease...

  37. Trends in CRISPR and SynBio Technologies

    Type
    Blog Post
    Published
    Feb. 4, 2015, 3:58 p.m.
    ...progress in all areas of research. We welcome your comments... Where do you think these technologies are going...

  38. The Codon Guide to Synthetic Biology

    Type
    Blog Post
    Published
    Sept. 14, 2023, 1:15 p.m.
    ... The video channels are also underrated and I recommend checking them out, especially since videos on ...

  39. Expanded Access to Rabies Plasmids

    Type
    Blog Post
    Published
    Feb. 1, 2024, 2:15 p.m.
    ...process takes 4-6 weeks on average. Therefore, we recommend placing your order well in advance whenever possible...

  45. Countdown to Halloween @Addgene

    Type
    Blog Post
    Published
    Oct. 29, 2014, 5:26 p.m.
    ...Addgene's favorite lunch... a BonMe noodle meal, crammed right into the box. Which is your favorite team...

  46. Podcast: Interview with Addgene's Founders

    Type
    Blog Post
    Published
    April 13, 2018, 12:51 p.m.
    ...UBMTA Chapter 4 - Developing Addgene as a modern e-commerce company and establishing credibility Chapter 5...

  48. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...snowflakes and viruses both exhibit similar forms of symmetry. 🎶Walking in a Winter Wonderlab🎶 We decided...

  50. Addgene’s Top 10 Blog Posts from 2014

    Type
    Blog Post
    Published
    Dec. 16, 2014, 8:01 p.m.
    ... Plasmids 101 series and by responding to your comments. Additionally we’ve seen what topics are of interest...
Showing: 201 - 250 of 481 results