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Looking for a plasmid that can thrive in a number of different bacteria? Learn about broad host range plasmids in this post!

April 11, 2024, 1:15 p.m.

If flow plots seem confusing, our guide to understanding them is here to help!

Feb. 6, 2024, 3:13 p.m.

Open science resources like Addgene's Data Hub increase reproducibility, reduce validation and optimization timeframes, and drive science forward.

Sept. 6, 2022, 1:15 p.m.

A new CRISPR/Cas9 system allows genome editing in all E. coli strains.

April 13, 2021, 1:15 p.m.

Lentiviral transductions can be frustrating. Follow these tips to achieve consistently successful lentiviral transductions.

April 7, 2016, 2:30 p.m.

Golden gate cloning can be used to efficiently and inexpensively clone multiple inserts into a single vector. Learn the basics of golden gate here.

Aug. 27, 2015, 2:30 p.m.

Addgene Executive Director Chonnettia Jones reflects on highlights of 2024 and shares exciting plans for 2025.

Feb. 25, 2025, 2:15 p.m.

This blog post explores how CRISPR/Cas9 can be used to visualize multiple intrachromosomal loci in vivo and map the 4D nucleome.

Aug. 11, 2015, 2:30 p.m.

Learn how and why AAV vectors are being used in cell and gene therapies.

Nov. 7, 2017, 1:59 p.m.

2024 is the Year of Camelids! To celebrate, we are taking a look at how these species (e.g. camels, llamas, alpacas) have impacted biological research.

May 9, 2024, 1:15 p.m.

SPARC is a tool that labels a fraction of a genetically-defined subset of cells. The result: easy visualization of individual, non-overlapping cells.

May 21, 2020, 1:15 p.m.

This post describes the biology and use of integrase-deficient lentiviral vectors in comparison to standard lentiviral vectors.

May 13, 2025, 1:15 p.m.

Learn how biotin is used in a variety of molecular biology experiments and makes it easier for researchers to label and purify macromolecules of interest.

Nov. 15, 2018, 1:50 p.m.

An overview of the Brainbow system and its variants and how they utilize Cre-lox for multicolor labeling of neurons.

April 24, 2015, 2:39 p.m.

The development of adenoviral vectors for the delivery of CRISPR/Cas9 expands genome engineering to primary cells.

Dec. 10, 2020, 1 p.m.

Use plasmids to develop synthetic nanobodies in a few weeks with a three step selection process.

June 18, 2020, 1:15 p.m.

Learn how to produce, purify, and titer adenoviral vectors for your research.

June 14, 2018, 11:44 a.m.

Researchers at the Wyss Institute for Biologically Inspired Engineering explain how you can use truncated gRNAs to regulate gene expression.

Jan. 10, 2017, 3:37 p.m.

Interview with Nicola Patron about her work in plant synthetic biology and her Golden Gate MoClo plasmid kit.

June 12, 2014, 3:30 p.m.

A review of a new brain to gut neural fiber technology from the Anikeeva and Bohorquez labs.

Aug. 31, 2023, 1 p.m.