...and a friend. George Church runs his lab fairly similarly to his advisor Wally Gilbert: “Very interdisciplinary...Institute (HHMI) offers some great resources for Early Career Scientists, including their "Making the Right... EMBO Lab Leadership Courses HHMI Resources for Early Career Scientists Science Careers: Running Your ...

...software. In fact, SnapGene’s feature library includes nearly 3x as many features as could be detected by our...extraneous ORFs that cluttered our old plasmid maps. Clearly Annotated Features, Enzymes, and Primers - The ...start thinking about your next cloning experiment early on. Beyond these simple but powerful display improvements...

...What fixed it was not trying even harder to start earlier, but simply moving all my prep work to the day ...would build a wall of pipette tip boxes when she particularly needed to focus, which was extremely effective... Guide to Getting Started in Undergrad Research Early Career Researcher Toolbox  8 Tactics for Choosing...

...antibody companies or check the literature. Pro tip! Early gates, like your live/dead markers or commonly used...isotype controls An isotype sample is prepared very similarly to a FMO sample: you make a sample from a combined...immunotherapies. She is currently a research technician in Arlene Sharpe's lab at Harvard Medical School.   More...

...the other hand, mScarlet-I shows correct ER labeling, which is good evidence that mScarlet-I acts as a monomer... situation, cellular assays have been proposed. Early efforts tested whether or not FP-tubulin fusion ...

...institutions doing after the injunction? We are particularly interested in whether institutions planning ...postdocs at other institutions to see if we can similarly convince them to go ahead with raises. What will...salaries. In addition, salary uncertainty may be particularly problematic for two-postdoc households. To better...

...Centro Ciência Viva" in Aveiro, Portugal. She is particularly interested in science journalism and writing...PMID: 15261139. Barsky, Sanford H., and Nina J. Karlin. "Myoepithelial cells: autocrine and paracrine ...absence of stromal caveolin-1 expression predicts early tumor recurrence and poor clinical outcome in human...

...that you think are clearly connected, step back and ask yourself, “are these clearly connected because ...you understand why you’re not communicating as clearly as you intended (it happens to us all!) and offer...

... in front of an audience and convey your ideas clearly and strategically is powerful in almost any career...and/or apprehensive. I still get nervous quite regularly, and I've had multiple jobs where all I did was...developed a valuable skill set that will be useful in nearly any other job you may choose to pursue. So take...

...in dsRNA generation. While these molecules are clearly associated with cellular damage and dysfunction... acid hybrids are always around. R-loops are particularly enriched in highly transcribed regions and repetitive...responses can mount in the absence of infection. Similarly, if endogenously synthesized dsRNA finds its way...

... in the final construct, allowing for precise, scarless cloning. The cloning scheme is as follows: the...but Golden Gate assembly can be designed to be scarless. Golden Gate assembly is also less expensive than...Marillonet. Golden Gate and genome engineering In early 2011, the Bogdanove and Voytas groups described ...

...exacerbated inflammatory response which leads to nearly 25% child mortality. Since glycans play an essential...amino acids more frequently than any other. Indeed, nearly 20% of the single missense mutant alleles that ...human disease change Arg to another amino acid. Clearly, the development of a pharmacological intervention...

...identical and all mice of the strain are homozygous at nearly all loci. This reduces variability in genotype ...reproducibility of experiments. Inbred mice are particularly useful for understanding a specific effect related...Examples of outbred strains include Swiss Webster and Harlan (Hsd) National Institutes of Health (NIH) Swiss...

...Sainsbury Laboratory. Are you a science student or early career researcher looking to break into science ..., Scientific American or National Geographic. Similarly, media fellowships from the British Science Association... Media Production at Imperial College London. Similarly, the University of Cardiff offers an MSc in Science...

...the accelerator of a vehicle being stuck down. Similarly, a mutation that turns a tumor suppressor “off...the Gateway Cloning Platform (Life Technologies, Carlsbad, CA) that represents 180 genes from the Ras signaling... 25877186. 4. Prior, Ian A., Paul D. Lewis, and Carla Mattos. "A comprehensive survey of Ras mutations...

...When Karl Barry Sharpless was awarded this year’s Nobel Prize in Chemistry, he joined an elite club, ... scientists to larger groups of trainees at an earlier point in their careers? A CURE for Biochemistry...protein with easily measured activity, MDH is particularly amenable to in-class labs. Provost ultimately...

...me). While things were incredibly busy in those early years, the founders knew that company culture and...throwing fun into the mix. Thus, our founders and early employees spent this remaining time by elaborately... you are planning, dangle the carrot (or more popularly- cookies). 3. Do something outside of your ordinary...

...scientific expertise. Organizing a career fair for early-career scientists provided a crash course in this...I find it useful to remind myself to check in regularly with collaborators for example on a holiday or...regard to reaching out to new people, checking in regularly, and maintaining useful notes that remind me about...

... interfering with downstream assays. This is particularly advantageous if the antibody used for IP is ...despite the size difference. Magnetic beads are particularly advantageous because they do not require centrifugation...binding proteins involved in histone modification. Similarly, RNA IP (RIP) can be used to isolate proteins ...

...markers tagged with mTurquoise2, mNeonGreen, and mScarlet-I for labeling a list of specific structures within...CRISPRa) are great ways to modulate the expression of nearly any gene via specific guide RNA (gRNA) sequences... New from the viral service By Ina Ersing We regularly add new viral aliquots from our plasmid collection...