Using Phosphoserine to Study Protein Phosphorylation
Type
Blog Post
Published
June 23, 2016, 2:30 p.m.
...postdoctoral fellow at the Morgridge Institute for Research in Madison, Wisconsin.
It is commonly cited that...perform in vitro kinase reactions. Often, however, researchers lack knowledge of the regulatory kinase, access... phosphoserine (as described below) provides researchers with a valuable tool for understanding the contribution...curious anomaly amongst a subset of methanogenic archea: these organisms lacked a classic cysteinyl tRNA... phosphoserine (Sep)-tRNA synthetase enabled researchers to create an orthogonal translation system (OTS...encoding the ORF to be expressed
Finally, the researcher needs a plasmid encoding their open reading frame...non-phospho-incorporated proteins purified from the system, researchers can run samples on SDS-PAGE gels containing Phos-Tag...