... advice for the group of juniors and seniors, including that it’s never too early to start networking!...was the favorite part of their visit for many, including Natalie Reid who said “Seeing the bacteria on ... of programming for scientists!). Careers that include both science and business or programming were new...showed some highlights of his travels from 2015, including a great European photo slideshow from our UK team...

...2015). This kit offers a broad range of parts, including copy number machinery, chromosomal integration...YTK and adds several additional capabilities, including conditional gene expression, multiplexed CRISPR...chromosomal integration and gene expression, the system includes a toolkit to express recombinant proteins within...kit plus the plant-specific parts you‘ll need, including sub-cellular localization signals, plant markers...engineering. This is a specialized system with parts including site-specific recombinases, regulatory elements...

... impact downstream outcomes of an experiment, including the accuracy and efficacy of findings. If you’...peptides and antibodies. Example applications of this include coupling to an antibody, whereby excitation of ...them under experimental conditions. Common issues include photobleaching, non-specific binding, and quenching...biotechnology. Recent examples of advancements include the Janelia Fluor(R) (JF) dyes developed by the...super-resolution applications. Other current advancements include work exploring far-red-emitting dyes in bacterial...

...selection screening. They and their colleagues including Feng Zhang used the GeCKO library to identify ...libraries developed by MIT's Tim Wang and colleagues, including David Sabatini and Eric Lander, comprise 73,171...different gene targets. These pooled libraries include enrichment against kinases, cell cycle proteins...designed to target a smaller set of genes and it includes more guides per gene. The Sabatini/Lander library...

...replication, and fluorescent proteins that are already included in Snapgene’s extensive database. Snapgene also...of open reading frames, which is often our first clue about where the gene of interest might be located...plasmids are difficult to sequence by NGS. These can include GC-rich and repetitive regions. These regions require... same features annotated in our plasmid maps, including our most popular primers and certain restriction...

...stays on the same page. Ideally, these meetings include all collaborators, from the lab members who are...like Zoom or Google Hangouts is a great way to include researchers from different locations. Having virtual...to your collaborators. This helps everyone feel included in the project and accelerates the pace at which...and morale. Especially when your collaboration includes scientists working in different time zones, it...

... and copy number of the plasmid) and typically include a gene that is advantageous for survival, such ...range of applications. Some common plasmid types include cloning plasmids, expression plasmids, gene knock-down...the many things that plasmids can be used to do include: Produce large amounts of a protein so that scientists...the lab. Addgene’s Molecular Biology Reference includes information about molecular cloning, how to choose...

... Now you’re wondering if your favorite gene is included in other CRISPR screens and whether that data ... each screen is captured by BioGRID curators, including the cell line/tissue type, enzyme system, sgRNA...-depth walk through of how to use ORCS:     Include your CRISPR screen data in ORCS  Researchers who...who wish to have their CRISPR screen data included in the BioGRID ORCS CRISPR database are encouraged ...

... lobes, REC and NUC, and their subdomains. NUC includes the HNH and RuvC catalytic domains, as well as...as the CTD (also known as the PI), while REC includes most of the bridge helix. Created with BioRender.com...the cleavage domains, the recognition domains—including the CTD and the entire REC lobe—do not structurally...lobes. The guide RNA in this structure does not include stem loop 3, and guide RNA nucleotides 11-20 are...discoveries on this topic are still being made! Conclusion Finally, we can summarize the process from start...

...building blocks including 14 different fluorescent proteins, 8 constitutive promoters, 2 includible promoters...Characteristics of the pHlash reporter protein include: H+ specific response cytoplasmic retention insensitivity...been used for a variety of experimental purposes including (1) single or multiple, constitutive or inducible...MXS-chaining were used in a variety of experiments including subcellular visualization of 6 different organelles...introduction of many different functional groups, including reactive groups for conjugation reactions, fluorescent... characteristics favorable for innocuous tags including high solubility, limited affinity for other proteins...mammalian cells with Broccoli Vegetable tags, including Spinach, Spinach2, and Broccoli, can benefit your...

...distributing lentivirus (with many CRISPR tools included among the preps that are currently available) ... AAV preparations have worked well in my lab, including in applications where we have delivered them in...The blog provides tips for working with viruses including advice on how to titer lentiviral preps, troubleshoot...on each viral prep’s specific material page and includes western blots as well as other expression data...

... browse our entire AAV inventory. Our new AAVs include: Optogenetics AAV Control AAV Biosensor AAV And...H134R) plasmids from Karl Deisseroth’s lab, which includes pAAV-hSyn Con/Fon hChR2(H134R)-EYFP that is both...have expanded our calcium sensor collection to include our first Flp-dependent GCaMP, AAV8-105714. Several... for the calcium sensor jGCaMP7 from Janelia, including three jGCaMP7 plasmids now packaged in AAVrg. ...

...span four continents and research diverse topics including microbiology, brain injury, drug discovery, and...PZA-resistance by using different approaches, including CRISPR and recombination-mediated genetic engineering...studied various aspects extreme life on earth including extremophile microorganisms, biomarkers, and recently...fluorescent signals from the sensor, and send a signal including its position in the river back to the user.   ...

...the ins and outs of this classification system, including its history, biology, and usages in the lab. History...peptidoglycan membrane. Common gram positive bacteria include staphylococci and streptococci. Gram negative bacteria... lipid membrane. Common gram negative bacteria include salmonella and E. coli. Because of their thicker... For more specific procedural instructions, including timing, preparing the smear, and mixing the stains...

...biological processes is highly useful in other fields, including synthetic biology. Addgene depositors Christopher...effectors are suboptimal for a number of reasons, including their potential toxicity and cross-reactivity ...level of “output” signal, was only about 10-fold, precluding the study of gene expression at very high or ...they can be used with many types of proteins, including Cas9 and dCas9-transcription factors, for which...

...ways foreign DNA can be introduced into cells including transformation, transduction, conjugation, and...often comes from adjacent lysed bacteria and can include plasmid DNA or fragmented DNA released into the...Addgene. For more information on viral vectors, including transduction download our Viral Vectors 101 eBook...into a eukaryotic cell through non-viral methods including both chemical and physical methods in the lab....

...Cancer Biology, where reported methods may not include how many tests were conducted on how the results...reporting guidelines can also help remind us to include all of the essential details. These items rarely...right before data collection starts, and should include a specific plan for how data will be collected,...and reduced bias in scientific research. These include policies for publishers and funders in the Transparency...

...controls are used in antibody-based applications including flow cytometry, immunohistochemistry, immunocytochemistry...recombination. Heavy chain constant genes are clustered together downstream of the variable region gene...they are the first constant domain genes in the cluster. However, when B cells switch to expressing a different... genes in the heavy-chain constant domain gene cluster. DNA recombination can delete intervening heavy-chain...

...GCE, each component of the experiment system (including media, cell type, and growth temperature) may ...to use a protocol that you’ll have to rework to include reagents accessible to your lab. The solution GCE...is for recombinant expression of phosphoserine-including proteins in E. coli (Zhu et al., 2022). It uses... While they’ve shortlisted several protocols, including their phosphoserine ncAA protocol, to optimize...

.... In the case of stem cells, these factors may include cell type, the disease being treated, and other...immunological, and environmental factors. How to include sex in experimental design Recognizing the importance...Institutes of Health (NIH) now requires that sex be included as a biological variable in all NIH-funded research...differences in effects on the sexes come from, one clue or potential place to start research is by considering...