...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes pAAV_hSyn-SIO-stChrimsonR-EGFP-P2A-PdCO-miniWPRE...What's new in Addgene's ready-to-use viral vectors repository? Quite a bit! In this post, we'll share the 20... 20 additions we've made to our viral vectors repository since March.  Addgene is now offering an AAV2...variants of a new optogenetic tool called PdCO, deposited by the Yizhar lab. Addgene is now offering AAV1...check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

...searching the Data Hub's curated collection of reports (which you can filter by serotype, species, or ... significant time and money. For instance, this report shows that anterograde, monosynaptic connections...our AAV Data Hub Challenge recording to see two reports presented by the submitting scientists, learn more...replicates of your experiment* Functional data, if the report describes a functional vector such as a channelrhodopsin...injection site as well as the target region, if the report describes a retrograde serotype An abstract that...experiment clearly and concisely* *required for a DOI. Reports that meet our minimum standards (* above) will ...

...labs how they can benefit from depositing their constructs to the repository, and getting feedback from our...Neuroscience Community blog to read reports from bloggers at the meeting – my report is currently in the works....Institute for Brain Research) – and an Addgene depositing scientist – stop by our booth to say hi! Zhang...successes. We've been lucky enough to meet many depositing scientists at #SfN14. And we've been so happy...

...Career Development; a Stakeholder Workshop Report.” The report enabled the development of priorities for...Development Hub Workshop Attendees and their 2021 report. Image from pd|hub.   To begin to tackle these...the findings were captured in this just released report “Enhancing Dissemination of Evidence-Based Models...programs.  Please join us for the pd|hub Workshop Report Public Release Event scheduled for March 4th from...2:30-4:00 pm ET.  You can learn more about the report's recommendations and join with us to discuss and...

...al., 2023). AAV1 capsids are available in the repository and can be selected as a serotype for Addgene...AAV2 capsids are available as plasmids in the repository and can be selected as a serotype for Addgene... targeting astroglia. It has additionally been reported to have tropism for lung, liver, vascular endothelial...al., 2023). AAV5 capsids are available in the repository and can be selected as a serotype for Addgene...AAV8 capsids are available as plasmids in the repository, as part of our in-stock viral prep service and...tissue tropism. AAVrg capsids are available in the repository and can be selected as a serotype for Addgene...Use Addgene’s AAV Data Hub to find experimental reports on commonly used vector cargo designs and administration...

...scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution service... we’ve curated microbiology plasmids from the repository onto one handy Microbiology Resource page and...Addgene’s plasmid sharing services, as well as #happydepositors who stopped by to say hello. Needless to say...amazing work already represented in the Addgene repository (like these new Rainbow Bacterial Fluorescence....org Check out Our Microbiology Resource Page Deposit Your Microbiology Plasmids with Addgene ...

...scientific community, contact deposit@addgene.org to find out more about our free deposit and distribution service...tumor and immune cells in real time. Addgene depositor Professor Max Krummel from UCSF showed some impressive...engineering (Ref 5)  as described by Blue Flame Addgene depositor Professor Michel Sadelain from the Memorial Sloan...potential to become an open-source pipeline and data repository for cancer researchers. Go open-source! Impressions...plasmids on her poster is pTomo-Ras which has been deposited at Addgene was used to produce the glioblastoma...

...the entire collection! Plasmid Serotype Name Depositor 27056 AAVrg pAAV-Ef1a-DIO EYFP Karl Deisseroth... expression of ChR2(H134R) bound to an mCherry reporter. We now also offer AAVrg for plasmid 83898, which...mDlx enhancer element. Plasmid Serotype Name Depositor 75470 AAV1 pAAV-CAG-FLEXFRT-ChR2(H134R)-mCherry...promotes neuronal firing. Plasmid Serotype Name Depositor 44361 AAV1 pAAV-hSyn-DIO-hM3D(Gq)-mCherry Bryan...advantage of the extensive supply of transgenic reporter lines that supply a variety of GFP expression ...also available in AAV1. Plasmid Serotype Name Depositor 69570 AAV1 pAAV-EF1a-N-CretrcintG Connie Cepko...

...scientific communities. The ARRIVE guidelines for the reporting of laboratory animals during in vivo research ...research and the CARE guidelines for case reports are two sets of guidelines frequently used by editors. Providing...s name and affiliations, and the entire referee report are permanently attached to the published article...and readers are able to comment on the referee reports. If the reviewers suggest that the manuscript requires...article, while the first version and the referee reports remain available online. Open-peer reviewing is...and effort) and the scientific community, as the reports can be very informative as to how a paper is received...

...highlight a subset of the new plasmids in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.          Listen to this episode...rapidly screen thousands of proteins for one that can report electrical activity via imaging. They took the ...subcellular localization processes in vivo. They deposited the resulting optogenetic plasmid tools which ...light- and dark- binder plasmid tools were also deposited for the photoswitchable AsLOV2. Find the cPYP...

...mild symptoms. This changed in January with the report of an outbreak of the virus in Brazil that was ...-ready format when the Zika virus outbreak was reported, and we felt that, given the critical need for...such a short timescale. Our article ultimately reported the development of a rapid design and prototyping..., targeting 24 regions of the genome, we have deposited the best performing sensors and constructs encoding...Find the Zika Sensor Constructs at Addgene As we report, we have embedded this next generation of toehold...Synthetic Biology Plasmids for Your Next Experiment Deposit Your Plasmids with Addgene ...

... plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles.  Here's ...gene disruption in M. abscessus  Bioluminescent reporter of mechanical forces   Epitope tag antibody ...Western Blot for Anti-V5 [IPI-SV5-Pk1]. Addgene Report, https://doi.org/10.57733/addgene.l0ulu4. Hanke...doi.org/10.1128/jb.00335-23.  Bioluminescent reporting of mechanical forces with PILATeS By Emily P. ...flexible! Alex Dunn’s lab and collaborators have deposited a new mechanosensor called PILATeS, Pulling-Induced... reaction (Figure 5). The researchers used the reporter to visualize biologically relevant forces at focal...

...subset of the new plasmids and viral preps in the repository through our hot plasmids articles. These articles...articles provide brief summaries of recent plasmid deposits and we hope they'll make it easier for you to ...you'd ever like to write about a recent plasmid deposit please sign up here.  Here's what you'll find in...the hPSC genome using AAV1 and contain an EGFP reporter. Expression of the dCas9-KRAB activator or dCas9...promoter. The gRNA piggyBac plasmids include a mRFP reporter. With this system, they could quantify and track...dCas9 targeting plasmids! Hazelbaker et al., Sci Reports. 2020. https://doi.org/10.1038/s41598-020-57500...

... plasmids, antibodies, and viral preps in our repository through our Hot Plasmids articles. Here's what... Bentley The David Liu Lab has made two recent deposits focused on optimizing prime editing systems for... PE-eVLP plasmids here! The lab’s other recent deposit builds on Prime-editing-Assisted Site-Specific ...Integrase Gene Editing (PASSIGE), their previously-reported technique for precisely integrating large DNA ...The Weissman and Gilbert Labs have previously deposited their CRISPRoff system — a programmable epigenetic...effectors (TALEs), with fifteen total variants deposited with Addgene. These variants, including a “miniCRISPR...

...scientists Benefits to depositing scientists: Access to a list of papers citing your deposited plasmids Increased...put, if the authors of a "Citing Article" have deposited plasmids associated with this "Citing Article"...cloning their gene of interest into a previously deposited vector - then you'll find the plasmids listed ...like to contribute plasmids you have made to the repository, email us and we'd be happy to help. Read More...

...primary antibody required. Reporters There are two main classes of reporters: chemiluminescence enzymes...you need a specific conjugate, and changing your reporter molecule requires either re-conjugating or re-...-purchasing your primary antibody with the new reporter. Finally, each new conjugate will have to be tested...film is only compatible with chemiluminescence reporters and requires access to a darkroom and a developer... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Protein-based... time; higher background Chemiluminescence reporter Smaller dynamic range; no multiplexing Wet...

...European scientists have deposited their plasmids into the Addgene repository. More than 4,500 plasmids...answer your questions about Addgene, plasmids, and depositing (all while our US counterparts are still sleeping...plasmids have already been deposited at Addgene by over 250 scientists in Europe. Despite these fantastic numbers...aware of what Addgene can do for them and how the repository can help them share their materials. The new ...

...viral vectors in the Addgene repository  Name Serotype Category Depositor  Notes AAV-GfaABC1D-Cre-...new preps available in the Addgene viral vector repository! This includes the first Addgene prep packaged...the Jonathan Ting lab at the Allen Institute, deposited as part of the BRAIN Armamentarium Collection....check out Addgene's Data Hub to see viral vector reports from other users. And don't forget to use the link...

...Allele Biotechnology which allows researchers to deposit plasmids containing the fluorescent protein mNeonGreen...fluorophores from Allele Biotechnology that now can be deposited at Addgene. What makes these fluorescent proteins...enhanced GFP (EGFP)-type termini. mNeonGreen was reported as the brightest monomeric green or yellow fluorescent... or for live cell imaging of fusions and FRET reporters. mTFP1 is also insensitive to physiologically ...to share with the scientific community, please deposit the plasmids at Addgene! References Ai, Hui-wang...

... plasmids in the repository, each verified by sequencing, which makes the repository a convenient source...genes deposited at Addgene were synthetic. But nine years later, over 20% of the genes deposited that ...most common expression system within the Addgene repository is mammalian, but the largest source of unique...