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We narrowed to 303 results for: GAI

Showing: 81 - 100 of 303 results

  1. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...form that can be understood by others, balanced against how often such data is likely to be accessed. Adding...natural, albeit unintentional, tendency to work against transparency. This opens science to charges of ...

  2. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...2015) or the UnaG protein that binds bilirubin (Kumagai, 2013). Next to these naturally occurring co-factor...biology 27 (2015): 39-45. PubMed PMID: 26087123. 4. Kumagai, Akiko, et al. "A bilirubin-inducible fluorescent...

  3. March for Science

    Type
    Blog Post
    Published
    April 21, 2017, 2:30 p.m.
    ...then revise their hypotheses and the cycle begins again. No part of this process is inherently political...scientists’ ability to uphold their side of the bargain and do the research required to inform the public...

  4. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...visualize their target. These antibodies are generated against a specific antigen and then conjugated with a fluorescent...that you will be able to image them in the end. Again, the methods you use will depend on target and sample...

  6. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ... experimental (?) groups were run on a western against a protein standard (P) to check for tau molecular...antibody specificity is to test the primary antibody against wild-type and knockout samples. In this test, wild-type...

  7. Viral Vectors 101: Optogenetic Tools

    Type
    Blog Post
    Published
    June 27, 2023, 1:15 p.m.
    ...light-driven pumps that pass ions (Cl-, H+, Na+) against the concentration gradient and hyperpolarize the...activation inhibitory (ArCH) proton pump passing ions against the concentration gradient. Created with BioRender.com...

  8. Viral Vectors 101: AAV Variables That Matter

    Type
    Blog Post
    Published
    June 1, 2023, 1:15 p.m.
    ...receptors, co-receptors, and other mechanisms to gain entry into the cell (Dudek et al., 2018; Korneyenkov...Moullier, P. (2002). Lack of an Immune Response against the Tetracycline-Dependent Transactivator Correlates...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...matter whether that other person really is acting against the team’s interests or not; once that seed of ...Guide Management for Scientists: Managing v Leading Gain Management Skills Volunteering at a Professional...

  10. Tips for a 1st Time CRISPR User (by a 1st Time CRISPR User)

    Type
    Blog Post
    Published
    March 7, 2017, 3:30 p.m.
    ...image. You could probably also do Western blotting against GFP expression, but I didn’t. 2. In the original...data not shown). Pro-Tips: 1.Designing primers against genomic DNA to amplify the gRNA targets was challenging...

  11. RNA Interference in Plant Biology: New Tools for an Old Favorite

    Type
    Blog Post
    Published
    Oct. 27, 2020, 1:15 p.m.
    ...the DNA backbone that contains a target sequence against the fluorescent reporter in an inverted repeat ...hairpin dsRNA is produced that contains sequence against your gene(s) of interest and the reporter. This...

  13. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ... In this movie Ethan Hawke’s character revolts against class divide that is created by genetics.    Zinc.... The movie sees the USS Enterprise crew fight against a genetically engineered superhuman Khan. The novel...

  14. Antibodies 101: Validation

    Type
    Blog Post
    Published
    March 24, 2022, 1:15 p.m.
    ...These strategies use multiple, unique antibodies against the same protein, each targeting a different epitope...correlation between the results. Using antibodies against different epitopes on the same target reduces the...

  15. Custom CRISPR Screens & the Green Listed Software

    Type
    Blog Post
    Published
    July 11, 2017, 2:30 p.m.
    ...are not looking for, but there are also benefits gained by lowering the complexity of the screen. For example...Another interesting approach is to design screens against known druggable targets, preferentially those for...

  17. 28 Hot Plasmid Technologies from 2015

    Type
    Blog Post
    Published
    Dec. 23, 2015, 3:30 p.m.
    ...steps and the joined modules cannot be separated again by the same cloning enzymes. MXS (MluI-XhoI-SalI...properties, they generated monoclonal antibodies against them, fused them to GFP, and compared them to popularly...In order to overcome these limitations, Takeharu Nagai and colleagues at Osaka University have developed...

  18. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...increase the chances of inducing an immune response against the capsids or transgenes. Sensory neurons and ... is not neutralized by pre-existing antibodies against AAV9-based vectors (and vice versa), enabling repeat...

  19. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ... to screen the NCC-1 library of approved drugs against the entire kit; a parallel analysis that successfully...sgRNA expression vectors (containing ten guides against each of the 8,158 predicted T. gondii protein-coding...

  20. 'Tis the Season to #DeckTheLab

    Type
    Blog Post
    Published
    Dec. 2, 2016, 3:30 p.m.
    ...creative scientists will deliver some fabulous photos again this year! Why #DecktheLab? Help us spread some...
Showing: 81 - 100 of 303 results