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We narrowed to 102 results for: Gene Tools Blue

Showing: 81 - 100 of 102 results

  1. To Each HIS Own

    Type
    Blog Post
    Published
    March 2, 2023, 2:15 p.m.
    ...interest (purple) and several contaminating proteins (blue, green, orange) flows through the Ni2+-containing...for antibody based assays. Antibodies are useful tools for studying proteins and are used for a variety...  Resources on addgene.org Addgene Antibody Guide Ready-to-use antibodies from Addgene More Resources ...Resources on the Addgene blog Plasmids 101: Protein Tags Antibodies 101: ChIP Antibodies 101: Introduction to ..., Bannwarth W, Döbeli H, Gentz R and Stüber D. Genetic Approach to Facilitate Purification of Recombinant...

  2. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...Resources on the Addgene Blog Find tips for online social networking for scientists Find tools for using social...connect with them in the future. Some profiles have a blue Follow button. For less famous people, this option...click this box, it will send an unpersonalized, generic invitation. Don’t try to connect from the LinkedIn...LinkedIn phone app either as this also results in a generic invitation. 3) Connect with people you have really...

  3. Making CRISPR Plasmids Using Fragmid

    Type
    Blog Post
    Published
    Aug. 29, 2024, 1:15 p.m.
    ...also deposit with Addgene!) We’re excited to help the Doench lab share this new tool and hope it helps...for stable fly cell lines; Hygromycin resistance gene; for genome integration by spontaneous insertion...that you can browse all components by clicking the blue text in the upper right-hand corner.      ...own Fragmid components! Ensure your backbone and genes contain only the Bbsl cut sites needed for the Fragmid...plasmids on the Addgene website. Step 4: Naming your assembled plasmid and generating the vector file....2024.100519 More resources from Addgene Plasmids 101: Modular Cloning Addgene's CRISPR Guide Plasmids 101: ...been looking for just the right CRISPR vectors on Addgene and found instead ones that were… pretty close,...

  4. FLEx Technology and Optogenetics: Flipping the switch on gene expression with high spatial and temporal resolution

    Type
    Blog Post
    Published
    Oct. 17, 2019, 12:28 p.m.
    ...Orientation)) is a powerful tool that provides precise temporal and spatial control of gene expression in vivo ...reporter gene (e.g., mCherry) to easily detect opsin-expressing cells. The resulting fusion gene is inserted...downside in optogenetics (Belzung et al., 2014). This could be overcome by fusing opsin genes to cell-type...Krook-Magnuson, 2018). Optogenetic FLEx vectors contain a strong promoter upstream of an opsin gene (e.g., channelrhodopsin...Bernstein, Jacob G., and Edward S. Boyden. "Optogenetic tools for analyzing the neural circuits of behavior...Yuan, and Feng Zhang. "Molecular tools and approaches for optogenetics." Biological psychiatry 71.12 (2012...Feng, et al. "The microbial opsin family of optogenetic tools." Cell 147.7 (2011): 1446-1457. PubMed PMID...

  5. Anatomy of a Plasmid Page at Addgene

    Type
    Blog Post
    Published
    Feb. 4, 2016, 3:30 p.m.
    ...within the gene/insert, this information can be found here. There may be more than one gene inserted into...can easily clone my gene into it? Your best bet is the Plasmid Map: We auto-generate a plasmid map based...example) and can be analyzed using Addgene’s Analyze Sequence tool. Excellent, how can I check if the... deposited with Addgene, you can list the original vector’s plasmid number here. Gene/insert: This describes...ordered more than 20 times; red, more than 50; and blue, more than 100 times! When you search for plasmids...the backbone, and provides information about this gene or DNA element of interest. If the depositor has... into a backbone, and each is given its own gene/insert section on the plasmid page. Tags are listed according...

  6. Cpf1 Update: Comparison to Cas9 and NgAgo

    Type
    Blog Post
    Published
    July 14, 2016, 2:30 p.m.
    ...directional gene transfer, analogous to traditional restriction enzyme cloning. Sticky-end mediated gene transfer...adeno-associated viral vectors) are commonly used for in vivo gene delivery due to their low immunogenicity and range...HAVE FAILED TO DEMONSTRATE GENOME EDITNG WITH THIS TOOL Cas9 and Cpf1 on-target and off-target editing efficiency...mismatches on modification by AsCpf1 (red) or LbCpf1 (blue). Mismatch positions are indicated below each graph... the potential “sticky end” transgene insertion mediated by Cpf1-generated overhangs. Exploring the efficiency...suited for genome editing.  Find Cpf1 Plasmids at Addgene   Image attribution: Zetsche, Bernd, et ...driven systems added another option to the CRISPR toolbox. When Cpf1 was first discovered, researchers wondered...

  7. A Primer on Optogenetics: Introduction and Opsin Delivery

    Type
    Blog Post
    Published
    Sept. 10, 2015, 2:30 p.m.
    ...in science, optogenetics is best used to address a specific question. Optogenetics is a tool and, just ... an optogenetics experiment and what are the tools required for each one? Behavioral Optogenetics can ... development of optogenetics, has genetically manipulated a number of the opsin genes to alter the light...era of neuroscience research where optogenetics will be a standard tool used in nearly any neuroscience ...But how exactly does an optogenetics experiment work? What are the various tools and components necessary...just like any other tool in a scientist’s vast toolbox, its effectiveness depends on how and why it’s used...PMID: 21692661.  3. Bernstein JG, Boyden ES. Optogenetic tools for analyzing the neural circuits of behavior...

  8. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ... palette for display (like green-magenta-blue or orange-blue rather than red-green). Even better, show...Immunofluorescence Resources on Addgene.org Find recombinant antibodies available at Addgene Protocols for Immunocytochemistry...antibodies and appropriate secondary antibodies. The general process is similar to standard immunofluorescence...IHC validated or ICC validated antibodies from Addgene), or be prepared to run some extra controls and...quantify accurately, but can be especially helpful to generate more signal from low abundance targets. Analysis...you find yourself stuck with red-green images generated automatically from the microscope software, you...three targets or you’re pushing the limits like Addgene depositor Ronald Germain (Radtke et al., 2020 multiplexed...

  9. Save Time with Transient Plant Leaf Transformations

    Type
    Blog Post
    Published
    July 25, 2019, 12:03 p.m.
    ...overexpressing a gene of interest in the plant. A common method for silencing genes Virus induced gene silencing...by replacing the genes on the T-DNA region with our gene of interest or a reporter gene so that the transformation...transformation. Choose good reporter genes There are many great reporter genes available and which one you choose...transformation success. Some reporter gene examples are below. The GUS gene (β-glucuronidase) with histochemical...Hikmet Budak. "Virus-induced gene silencing, a post transcriptional gene silencing method." International...stable cell line or plant is regenerated from the transformed tissue. Gene function is commonly studied...via Addgene. For us, transient leaf transformations are a great help in screening candidate genes and ...

  10. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...possible to build gene regulatory RSMs (GRSMs, the subset of RSMs that regulate gene expression) that ...large serine recombinase genes downstream of inducible promoters: the BxbI gene downstream of the anhydrotetracycline...the TP901 gene downstream of the arabinose (Ara)-inducible promoter (PBAD), and the A118 gene downstream...register to execute predictable gene regulation programs that specify which genes should be expressed or not... same gene regulation program. We recommend testing multiple register designs for the same gene regulation... paper. The paper also demonstrates that genetic parts (genes, terminators, and promoters) may be interweaved...register that expresses a unique combination of red, blue, and green fluorescent proteins in each state. ...

  11. Four Base Editing Reporters to Monitor and Enrich Editing in Real-time

    Type
    Blog Post
    Published
    July 7, 2020, 1:15 p.m.
    ... and adaptable tool to identify and enrich for base-editing events in vivo. TLDR: Gene On (GO) from the...integrated GFP reporter that is converted into BFP (blue fluorescent protein) upon CRISPR/Cas9 homology directed...edits of interest than solely transfected cells. “Gene On” (GO)- a functional reporter system to identify... (Katti et al., 2020). They named this reporter “Gene On” or GO. GO works by affecting protein translation...fluorescent proteins. Figure 4: Reporters in Gene On include a mScarlet reporter, luciferase reporter...base-pair mCherry insertion. Editing of these motifs generates lesions that are subject to excision and ssDNA...This edit changes the histidine to a tyrosine generating a GFP variant (GFPY66) that has a shifted emission...

  12. Antibodies 101: Introduction to Antibodies

    Type
    Blog Post
    Published
    Jan. 19, 2021, 2:15 p.m.
    ...are both a natural part of the immune system and a tool that can be used for a variety of research applications...which come from naturally-occurring immunoglobulin genes, recombinant antibodies are plasmid-based. Their...staining for CD31 (green), CARD14 (red), and pNFkB (blue) in lesional skin from a psoriasis patient, showing...express antibodies, nanobodies, and ScFvs, as well as tools for developing and producing plasmid-based antibodies... What is an Antibody? Resources on Addgene.org: Browse Addgene’s Antibody Plasmid Collection Find antibodies...cascades that clear these infections. Anything that generates an antibody response in the immune system is referred...in the DNA of the hybridoma can occur, known as genetic drift, meaning that over time, the antibody you...

  13. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ...Near-infrared fluorescent proteins, biosensors, and optogenetic tools engineered from phytochromes." Chemical reviews...PMCID: PMC3783214.  10. Hilaire, Mary Rose, et al. "Blue fluorescent amino acid for biological spectroscopy...expected to grow with the ever-expanding genetic toolbox generated by the continuous effort of numerous scientists...). GFP is the most popular, most widely used genetically encoded fluorescent probe. Several factors contribute...probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are, however, ...1998). Another mutation (A206K) is necessary to generate the strictly monomeric EGFP variant, mEGFP (Zacharias..., followed by an intramolecular reaction that generates the chromophore (Tsien, 1998). Importantly, this...

  14. Hot Plasmids - November 2023

    Type
    Blog Post
    Published
    Nov. 14, 2023, 3:35 a.m.
    ...delivery of genetic cargo  By Alyssa NeuhausTo improve delivery and reduce off-target effects of gene therapy...) LOV-Turbo becomes active when illuminated with blue light, catalyzing the biotinylation of nearby proteins...system to improve on-target delivery of genetic cargo Optogenetic proximity labeling and TransitID Tracking...continuous evolution and protein engineering to generate seven new prime editors, PE6a–g, that overcome...10.1038/s41467-023-40788-8. PMID 37612276.   Optogenetic proximity labeling and TransitID By Mike Lacy...transcriptional history of a cell. Lin et al. generated a fusion protein called iPAK4 that assembles into...43R] has been one of the hottest antibodies in Addgene’s collection since we began distribution and has...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...improved AAV vectors could also be powerful tools for the field of gene therapy if they can reach the affected...Hybrid Serotypes: Improved Vectors for Gene Delivery. Curr Gene Ther 5(3), 299–310. PubMed PMID: 15975007...Tonegawa S. (2014). Cell type-specific genetic and optogenetic tools reveal novel hippocampal CA2 circuits...several virus species have been modified and used as tools for tracing. With some engineering, RABV and HSV... few different fluorescent proteins (red, green, blue). If a researcher injects a cocktail of these different...anterograde and retrograde axonal transport. Hum Gene Ther 25, 705–720. PubMed PMID: 24694006. PubMed ...2017). Engineered AAVs for efficient noninvasive gene delivery to the central and peripheral nervous systems...

  16. Typing CRISPR Systems

    Type
    Blog Post
    Published
    March 18, 2025, 1:15 p.m.
    ...domain due to a gene that resembles cas10. All type IV subtypes are missing adaptation genes, Cas1 and Cas2...sequence similarity, phylogenetic analysis, neighborhood analysis of surrounding genes, and experimental ...straightforward as it seems. There is not a single gene that is shared across all CRISPR types; the fast... to the multi-Cas effector, Cascade. Developing tools using a Cascade complex is tricky, as you need to... Figure 2: Schematic of a Cascade complex (blue subunits) with a guide RNA (green and orange) bound...together because they contain a distinct Cas7-type gene. The exact mechanism of type IV systems is not known... and applications with the type I CRISPR system. Gene and Genome Editing, 3–4, 100013. https://doi.org...

  17. Five Popular Model Organisms, Part 2

    Type
    Blog Post
    Published
    Sept. 29, 2020, 1:15 p.m.
    ...and after embryogenesis. Addgene has over 600 plasmids that contain Xenopus genes and a tool-kit from ... a vast array of molecular tools, such as gene manipulation and mutagenesis techniques, are available ...requiring a variety of biological and molecular tools. One such tool is the model organism, an essential aspect...human genes have counterparts in the rat (Gibbs, et al., 2004). Additionally, breakthroughs in gene modification...abundance of genes related to limb regeneration (Vieira et al., 2019). To study regeneration scientists ...molecular biologist tool box” for its common use in plasmid manipulation and genetics research. Plasmids...Plasmids are ideal tools for studying genetic modification for their ease of manipulation and their ability ...

  18. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...   Conclusion Addgene is a great example of how sharing of data and molecular tools can lead to faster... years, the light microscope has been a powerful tool in almost all disciplines. In biology, innovative...Diederich et. al., 2020) aim to make microscopy as a tool accessible to practically everyone.     ...development process. Widely used, commercially available tools such as the Raspberry Pi, the Arduino, or 3D printers...work. Sharing open-source plans that rely on such tools has allowed the community to regularly replicate... based around the use of commercially available tools and components. Rapid prototyping techniques such...OpenFlexure microscope or open-source modular optical toolbox openUC2 are trying to overcome this hurdle by taking...

  19. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ...to expand our educational content supporting the tools in our collection, such as blogs describing viral...accessibility, we're equipping every scientist with the tools they need to push the boundaries of discovery. As...power of community in 2024 In 2024, your trust in Addgene helped unlock unprecedented scientific collaboration...sequencing capabilities to complement our next-generation sequencing, setting new standards for plasmid...online and in-person, you helped us understand how Addgene can better serve the global scientific community...researchers worldwide. Through our growing community on Bluesky, our expanding educational resources, and our commitment...about our latest developments? Subscribe to the Addgene newsletter. References Ayoubi, R., Ryan, J., Gonzalez...

  20. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...an unpublished set of Zika gene plasmids to Addgene’s repository. The genes, from the Zika virus Asian...vectors targeting their genes of interest to generate mAID fusions to endogenous genes. Cell lines with the...targeting a total of 20,121 genes within the human genome (the identity of the genes and target sequences can...expression level for their gene of interest. Although the parts involved in altering gene expression are relatively...contains ~90,000 gRNAs that target ~15,000 genes with 6 gRNAs per gene. The second is a "supplementary" library... domain is an incredible tool for researchers trying to activate target genes, the relatively large size...library to generate a population of HEK293FT cells expressing a single gRNA targeting a single gene. The gRNA...
Showing: 81 - 100 of 102 results