We narrowed to 378 results for: Hal
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TypeBlog PostPublishedJan. 6, 2015, 5:21 p.m.... et al., Science. 2014. Jan 3;343(6166):80-4. Shalem et al., Science 2014. Jan 3;343(6166):84-7. Koike-Yusa...identified a family of GFP proteins in the cephalochordate Branchiostoma floridae, named bfloGFPs. They...implicated in disease progression. One of the major challenges in signaling research is the inability to replicate... (including His, S-tag, OneStrep, CBP, Trx, GST, Halo, MBP, NusA and SUMO) for optimizing expression, ...
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Finding Your Perfect Job After University
TypeBlog PostPublishedJan. 12, 2016, 3:30 p.m....become bored because I was not being mentally challenged. For these and other reasons, I started looking...combination of genetic mutations. This is a particular challenge in pancreatic cancer where one drug does not work...while I’m based in London) does bring its own challenges. I’ve had to become skilled with technology like... -
Finding Your Science Policy Path
TypeBlog PostPublishedSept. 22, 2020, 1:15 p.m....That’s what makes it so wonderful. The most challenging part of jumping into this career path from academia... your hair out, your successes, failures, and challenges you’ve overcome. This information may help you...completely new field without issue. This may be more challenging if you are trying to apply for an outright job... -
With an Eye Towards the Future, We Look Back at the March for Science
TypeBlog PostPublishedOct. 24, 2017, 1:54 p.m....the University of California Berkeley. It’s been half a year since the march for science on April 22, ...present at the lab bench," Liu, a UM undergraduate, challenged us. I worried the march would be a simple, unquestioning... fingers of skepticism to defend an agenda that halts current data collection, destroys existing data,... -
Fluorescent Proteins 101: Monitoring Cell Mobility Using Fluorescent Proteins
TypeBlog PostPublishedAug. 15, 2017, 1:24 p.m.... lymph nodes at different times after antigen challenge, scientists were able to track where GFP labelled...et al. 2016, Cavanagh et al. 2008)? Technical challenges of two photon microscopy Intravital imaging is... at steady state or under physio-pathological challenge. However technical specificities have to be taken... -
Custom CRISPR Screens & the Green Listed Software
TypeBlog PostPublishedJuly 11, 2017, 2:30 p.m....that code for surface proteins. One of the first challenges with a custom screen approach is to identify ...PMCID: PMC4744125. 3. Sanjana, Neville E., Ophir Shalem, and Feng Zhang. "Improved vectors and genome-wide...essentiality profiling reveals gene networks and synthetic lethal interactions with oncogenic Ras." Cell 168.5 (2017... -
SciComm with the Experts at Science in the News Part 1
TypeBlog PostPublishedMarch 16, 2017, 1:06 p.m.... This is the first half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...eager to communicate their science. In this first half of our two-part interview with Vini and Amy, we’... -
Measuring Kinase Activity at the Single-Cell Level with Kinase Translocation Reporters (KTRs)
TypeBlog PostPublishedJuly 26, 2018, 12:46 p.m....kinase activity is FRET, but FRET reporters are challenging to design and difficult to multiplex. The Covert...which fluorophores can be multiplexed together. Challenging to Design: FRET reporter activity depends on... -
Scientist Networking: What is an Informational Interview?
TypeBlog PostPublishedJuly 1, 2014, 3:42 p.m.... about salary, opportunities for advancement, challenges and disappointments. These more personal questions...people honor. Talking with strangers is usually challenging but does get easier with practice. Another perk... -
X-CHIME: Context Dependent Germline Knockout in Immune Cells
TypeBlog PostPublishedJan. 16, 2024, 6:13 p.m.... cancer immunotherapy targets but are embryonic lethal, hindering studies post embryogenesis. Using C-... in a developing or developed immune system was lethal (LaFleur et al., 2024). Figure 1: Schematic... -
SciComm with the Experts at Science in the News Part 2
TypeBlog PostPublishedApril 27, 2017, 2:30 p.m....This is the second half of a two-part interview with Vini Mani and Amy Gilson from Science in the News...involved in science communication. In this second half of our conversation with Vini Mani and Amy Gilson... -
Inside Addgene's 10 Year Anniversary Gala
TypeBlog PostPublishedJuly 3, 2014, 3:48 p.m....that Addgene employees created and designed for the Hall of Human Life at the MOS. (If you want a fun refresher... ghost writers, of course! Amy Uddin and Emma Gottschall, two of Addgene's lab technicians, gave Blugene... -
Addgene @ Keystone: Thoughts on Precision Genome Engineering and Synbio
TypeBlog PostPublishedJan. 15, 2015, 1:50 p.m....systems, modifications to the RNA components, and challenging target genomes, such as the malaria-causing Plasmodium...Addgene depositor, discussed some of the many challenges of creating biological circuits, and I can only... -
Arabidopsis in Education: How the Arabidopsis Biological Resource Center Brings Plant Science to Life
TypeBlog PostPublishedApril 12, 2018, 1:02 p.m....one of two global stock centers for Arabidopsis thaliana (Arabidopsis). Our mission is to collect, preserve...system comes with fewer ethical and logistical challenges than does working with animals. Because Arabidopsis... -
Behind-the-scenes of the Isolation of the Thermostable IgnaviCas9 From a Yellowstone Hot Spring
TypeBlog PostPublishedNov. 12, 2019, 2 p.m....Schiebinger, another Quake Lab member joined for half of the trip. Preparations ahead of time included...spring requiring an overnight hike in. Navigating challenges: collecting and processing environmental samples... -
Antibodies 101: Avoiding the Mouse on Mouse Mess in IHC
TypeBlog PostPublishedSept. 21, 2023, 1:15 p.m....staining can be a challenge in the best of cases. But it can be particularly challenging when your only ... -
Hot Plasmids: FRET-Based Biosensors
TypeBlog PostPublishedMay 6, 2014, 1:07 p.m....problem that he considers “one of the greatest challenges of neuroscience.” Hot plasmids: Twitch sensors...trial and error. Transition metals also present challenges in that they are found in cells at much lower... -
"Build Your Plasmid": The Game - Play to Teach
TypeBlog PostPublishedJuly 17, 2018, 12:23 p.m....Associate Professor at University of Barcelona. The challenge: Making courses on genetic manipulation more hands...could be played when and, depending upon who your challenger was, you might have to drastically alter your... -
Reagent Repositories Are Speeding up Science During the Pandemic
TypeBlog PostPublishedAug. 4, 2020, 1:15 p.m.... J, Jia HP, Halabi C, Sigmund CD, Meyerholz DK, Kirby P, Look DC, Perlman S (2006) Lethal Infection of... -
25 Work From Home Tips for Scientists
TypeBlog PostPublishedMarch 19, 2020, 1:15 p.m....exploration site Inventing Heron has a radio reporter challenge, and code.org has coding tutorials for all ages...environment. Be kind to yourself. This is a challenging period for everyone for numerous reasons, and...