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We narrowed to 144 results for: INV-E

Showing: 81 - 100 of 144 results

  1. A History of Genome Engineering in Popular Culture

    Type
    Blog Post
    Published
    Feb. 25, 2020, 2:15 p.m.
    ...10.1534/genetics.111.131433 Doudna JA, Charpentier E (2014) The new frontier of genome engineering with...CS, Ralston EJ, Lee AH, Amora R, Miller JC, Leung E, Meng X, Zhang L, Rebar EJ, Gregory PD, Urnov FD, ...testing and modifications. The premise of the novel involves Dr. Moreau who creates human-animal hybrids. This... the discovery of meganucleases. The storyline involves the resurrection of dinosaurs by extracting the...Tom Hanks as the protagonist (Langdon). The plot involves Langdon chasing an anti-villain geneticist named...which goes horribly wrong. The plot of the movie involves The Rock and his super giant albino gorilla fighting...

  2. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...greatly simplified the process of generating multiple E. coli gene expression plasmids with the Cross-disciplinary...gate cloning that results in the creation of many E. coli constructs with greatly varied levels of expression...well as a dominant-negative mutator allele of the E. coli MMR protein MutL, all under the control of the... MutL is highly conserved in distant relatives of E. coli so it can be used in a broad range of strains...domains, but these domains often do not express well in E. coli without their partner regulatory domains. The...library and used this library to screen for genes involved in West Nile Virus (WNV) induced cell death. The...for their gene of interest. Although the parts involved in altering gene expression are relatively well...

  3. Four Ways to Package Transgenes That Exceed the Size Limit of Adeno-associated Virus

    Type
    Blog Post
    Published
    Sept. 1, 2020, 1:15 p.m.
    ... Sommella A, Iodice C, Cesi G, Simone S, Marrocco E, Rossi S, Giunti M, Palfi A, Farrar GJ, Polishchuk...doi.org/10.1002/emmm.201302948 Trapani I, Toriello E, de Simone S, Colella P, Iodice C, Polishchuk EV, ...results in effective gene delivery in mice. J Clin Invest 118:1955–1964 . https://doi.org/10.1172/jci34316...

  4. Generating Mouse Models Using CRISPR/Cas9

    Type
    Blog Post
    Published
    July 12, 2016, 2:30 p.m.
    ....org/10.1126/science.1231143 Doench JG, Hartenian E, Graham DB, Tothova Z, Hegde M, Smith I, Sullender...doi.org/10.1038/s41598-018-33408-9 Mizuno N, Mizutani E, Sato H, Kasai M, Ogawa A, Suchy F, Yamaguchi T, Nakauchi...easy. In the 1980s, gene targeting technology was invented to introduce specific changes into the mouse genome...

  5. Reflections and Looking Ahead

    Type
    Blog Post
    Published
    Feb. 25, 2025, 2:15 p.m.
    ..., A. I., Saper, C. B., Errington, T. M., Turn, R. E., Bandrowski, A., Trimmer, J. S., Rego, M., Freedman... plasmids — as DNA or bacterial stabs — ‌and reinventing our search experience so you can find exactly...

  6. Plasmids 101: Positive and Negative Selection for Plasmid Cloning

    Type
    Blog Post
    Published
    Aug. 22, 2019, 12:43 p.m.
    ...synthesizes the critical amino acid). Many common E. coli strains used for cloning are auxotrophic for.... Fortunately, there are many ways to do this involving positive selection, negative selection, and/or...

  7. Sleeping Beauty Awakens for Genome Engineering

    Type
    Blog Post
    Published
    June 30, 2015, 2 p.m.
    ...grow in the coming decades.  References 1. Kowarz E, et al. Optimized Sleeping Beauty transposons rapidly...) The transposon consists of a mirrored set of inverted repeats (green) flanking the gene of interest ... enzyme is expressed (red stars) and binds the inverted repeats (green); and an endonuclease reaction ...

  8. Genetically-encoded Sparse Cell Labeling - A SPARC of Innovation

    Type
    Blog Post
    Published
    May 21, 2020, 1:15 p.m.
    ...Figure 2: Dense (c), intermediate (d), and sparse (e) labeling of the calcium indicator, GCaMP6f, in the...makeup, overcomes all of these labor-intensive and invasive techniques to reliably label any post-mitotic ...

  10. Bright Monomeric Fluorescent Proteins: mNeonGreen, mTFP1, and mWasabi

    Type
    Blog Post
    Published
    April 25, 2019, 3:01 p.m.
    ...University of Oregon created mTFP1 by transforming E. coli with a gene library of approximately 484 variants...fluorescent protein (LanYFP) from the marine invertebrate Branchiostoma lanceolatum in 2012. Using structural...

  11. What the HEK?

    Type
    Blog Post
    Published
    April 18, 2023, 1:15 p.m.
    ...2002) (8):869-71. DOI: 10.1096/fj.01-0995fje Tan, E., et al., HEK293 Cell Line as a Platform to Produce...interests include receptor biology, ion channels, or involve human protein preparations with PTMs. Why the HEK...

  14. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...website, social media, videos, newsletters, guides, and e-books – comes into play. Our goal is to provide informative...post, we invite you behind the scenes in our content writing process, and to even get involved yourself...standards. It’s always a work in progress! Get involved A lot of our content already comes from you and...depositors and customers are asking. So how can you get involved? Keep asking your burning questions and tell us...

  15. Hot Plasmids and Viral Preps - September 2021

    Type
    Blog Post
    Published
    Sept. 21, 2021, 1:15 p.m.
    ...puromycin resistance.  The MYC-CRISPR library targets E-boxes genome-wide. It was designed based on MYC-ChIP-seq...lab targets human genes known or suspected to be involved in DNA damage response and DNA repair.  A new ...

  16. Multifunctional Neural Fibers: Modulation from Gut to Brain

    Type
    Blog Post
    Published
    Aug. 31, 2023, 1 p.m.
    ...10.1039/C8CS00710A Sahasrabudhe, A., Rupprecht, L. E., Orguc, S., Khudiyev, T., Tanaka, T., Sands, J., ...animals being monitored were physically hooked up to invasive, cumbersome systems during experiments – neither...

  17. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...Standards Synthetic Photobiology: Optogentics for E. coli Browse All Synthetic Biology Blog Posts Resources... into modules that students and teachers can investigate together. Teachers decide which BioBuilder lessons...of teachers around the country. How you can get involved with BioBuilder Details for joining BioBuilder...

  18. Hot Plasmids December 2018 - C. elegans RNAi, TurboID, Detecting Viral Outbreaks, and Protein Binding Specificity Tools

    Type
    Blog Post
    Published
    Dec. 6, 2018, 1:34 p.m.
    ...gene function. RNAi is commonly achieved by feeding E. coli expressing dsRNA to C. elegans. These dsRNAs...vector, L4440, places a target gene between two inverted T7 promoters. Transcription from each promoter...Src homology 3 (SH3) domain family, known to be involved in cytoskeletal control, cell growth, and endocytosis...carry out any biochemical and biophysical study involving protein stability or binding experiments. Potential...

  19. Antibody Validation for Flow Cytometry

    Type
    Blog Post
    Published
    Sept. 10, 2024, 1:15 p.m.
    ..., Carr, S., Edwards, A., Ellenberg, J., Lundberg, E., Rimm, D. L., Rodriguez, H., Hiltke, T., Snyder, ...M. S., Smallwood, D. T., Weston, C. A., Castells, E., Bowman, V. W., McCarthy, J., Amrani, Y., Duffy, ...not in the iso-genic knockout control. This can involve cell surface labeling and/or intracellular labeling...overexpress the target of interest. This approach often involves the transient transfection of expression plasmids...

  20. Hassle-free 96-well Format Epitope Tagging Using Cas9 Ribonucleoprotein

    Type
    Blog Post
    Published
    June 28, 2018, 3:01 p.m.
    ...References 1. Bressan RB, Dewari PS, Kalantzaki M, Gangoso E, Matjusaitis M, Garcia-Diaz C, Blin C, Grant V, Bulstrode..., Southgate B, Mccarten K, Monogarov G, O'Duibhir E, Quinn N, Tyrer A, Leitner MC, Plumb C, Kalantzaki...purchased directly from commercial suppliers without investing time and labour on reagent preparation in the ...
Showing: 81 - 100 of 144 results