We narrowed to 254 results for: IRES

.... Let’s imagine you’re applying to a job that requires ddPCR skills. Your resume lists ddPCR, so the ...application. Even so, there were examples of great hires who hadn’t submitted a cover letter; their experience...

..., BioID2 exhibits enhanced proximal labelling, requires less biotin, and allows scientists to more selectively...APEX2, although it can rapidly label proteins, requires H2O2 which can be toxic to living cells. To circumvent...

...composition of the RISC varies, but at the very minimum requires an Argonaute protein. This Argonaute protein is...shRNAs using plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence...

...look like (the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great...edits and look for off-target changes. This method requires a set of control cells to compare the sequencing...

... DNA outside of the recognition sequence. This requires that the vectors and DNA fragments contain these...sites recognized by the integrase. This method requires specialized vectors containing these integration...

...difficult to isolate single registers or if it requires reading so many registers that Sanger sequencing...minimal cellular perturbation. Also, this method requires live, intact cells; the sequencing and qPCR methods...

...writing can take weeks to a couple of months and requires that all of the experiments have been completed...thoughtful engaging talk takes significant effort. It requires evaluation of all of the data and any recent relevant...

...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical...

...world, so a process or project or mission that requires a broad knowledge base is going to necessarily...the type of team. Knowing what roles your team requires to function is certainly necessary, but equally...

...'t require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic) Digenome...doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to time since ...

...one since the formation of mEosFP’s chromophore requires a temperature below 30°C and this is not ideal...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve cellular...

...spectrophotometry. Achieving the highest-quality DNA requires taking care at each step, but some purification... Using non-silica-based purification protocols requires less plastic and avoids the use of more hazardous...

...the R-loop structure. In order to bind DNA, Cas9 requires (1) a guide RNA, (2) a complementary DNA sequence...maybe because repairing a GBE-derived abasic site requires more cellular factors binding to DNA. However,...

...national interest waiver criteria however the EB-1A requires a higher standard of proving that you are one ...people engaged in researcher activities. The EB-1B requires that you provide two out of six forms of documentary...

...neuronal tracing compounds and this is an area that requires further study. Traditional neuronal tracing methods...for neuronal tracing and network identification requires both antero- and retrograde labelling, synaptic...

...on or off the expression of shRNA. This system requires less work than using established methods and minimizes...thorough understanding of complex biological systems requires both a catalog of molecular players and contextualized...

...biosafety cabinet ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene...

...using this PEI Calculator. AAV packaging typically requires transfecting three plasmids at specific molar ...

...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ...

...biomass. Dr. Duedu would like to use the plasmids he acquires through Addgene to produce molecular biological...