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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 253 results for: IRES

Showing: 81 - 100 of 253 results

  1. AAV Titers: Where Do They Come From And What Do They Mean?

    Type
    Blog Post
    Published
    Nov. 15, 2017, 3 p.m.
    ...solution. Since this technique is intensive and requires an electron microscope, it is not routinely performed...a few ways:  Absolute quantification by qPCR requires generating a standard curve of known concentration...

  3. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...look like (the 3rd sequencing trace). While it requires a little extra wet lab work, this is still a great...edits and look for off-target changes. This method requires a set of control cells to compare the sequencing...

  4. Plasmids 101: Expressing shRNAs for RNA Interference

    Type
    Blog Post
    Published
    June 17, 2025, 1:15 p.m.
    ...composition of the RISC varies, but at the very minimum requires an Argonaute protein. This Argonaute protein is...shRNAs using plasmids The design of shRNA plasmids requires three important steps: Designing the shRNA sequence...

  6. Technologies Enabled by NanoLuc® Luciferase

    Type
    Blog Post
    Published
    Feb. 8, 2018, 12:17 p.m.
    ...for how sensitive you can make a reaction. NLuc requires 100-fold less protein to produce the same amount...detecting traditional luciferases’ dim light emissions requires long acquisition times that may be impractical...

  8. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...writing can take weeks to a couple of months and requires that all of the experiments have been completed...thoughtful engaging talk takes significant effort. It requires evaluation of all of the data and any recent relevant...

  9. Transferable Skills Guide: Teamwork

    Type
    Blog Post
    Published
    Nov. 28, 2017, 2 p.m.
    ...world, so a process or project or mission that requires a broad knowledge base is going to necessarily...the type of team. Knowing what roles your team requires to function is certainly necessary, but equally...

  10. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...'t require high read counts, fast multiplexing Requires delivery of dsODN (potentially toxic) Digenome...doesn't require high read counts (fast multiplexing) Requires large cell population, senstive to time since ...

  11. Fluorescent Proteins 101: Photoactivatable Fluorescent Proteins

    Type
    Blog Post
    Published
    April 25, 2017, 2:30 p.m.
    ...one since the formation of mEosFP’s chromophore requires a temperature below 30°C and this is not ideal...fluorescence localization data. However, this technique requires harsh fixation conditions to preserve cellular...

  12. Selecting Your Plasmid Purification Kit

    Type
    Blog Post
    Published
    May 27, 2025, 1:15 p.m.
    ...spectrophotometry. Achieving the highest-quality DNA requires taking care at each step, but some purification... Using non-silica-based purification protocols requires less plastic and avoids the use of more hazardous...

  13. CRISPR 101: Cytosine Transversion Editors

    Type
    Blog Post
    Published
    March 25, 2025, 1:15 p.m.
    ...the R-loop structure. In order to bind DNA, Cas9 requires (1) a guide RNA, (2) a complementary DNA sequence...maybe because repairing a GBE-derived abasic site requires more cellular factors binding to DNA. However,...

  15. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...neuronal tracing compounds and this is an area that requires further study. Traditional neuronal tracing methods...for neuronal tracing and network identification requires both antero- and retrograde labelling, synaptic...

  16. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...on or off the expression of shRNA. This system requires less work than using established methods and minimizes...thorough understanding of complex biological systems requires both a catalog of molecular players and contextualized...

  17. Bringing Renewable Energy to Addgene

    Type
    Blog Post
    Published
    Aug. 27, 2019, 1:08 p.m.
    ...biosafety cabinet ventilation, and lab lighting/HVAC requires a significant energy expenditure here at Addgene...

  19. Celebrating One Million Plasmids Shared!

    Type
    Blog Post
    Published
    Sept. 25, 2018, 2:39 p.m.
    ...would be an average of 673 plasmids each day. This requires tremendous coordination amongst the lab team, ...
Showing: 81 - 100 of 253 results