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We narrowed to 461 results for: MEA;

Showing: 81 - 100 of 461 results

  1. Sequencing Options for CRISPR Genotyping

    Type
    Blog Post
    Published
    Oct. 4, 2016, 2:30 p.m.
    ...They also have a limit of detection of ~5%. This means they do not reliably detect editing events that ...population (Fu et al. 2013, Vouillot et al. 2015). Meanwhile, Sanger sequencing is laborious, time consuming...as .01% (Hendel et al. 2015). Low detection rates mean the investigator can be relatively certain that ...the genome. However, their decreased sensitivity means that the best option moving forward may be to integrate...

  2. Antibodies 101: Designing Your First Flow Panel

    Type
    Blog Post
    Published
    May 28, 2024, 1:45 p.m.
    ...cells using flow cytometry, you are typically measuring the presence or absence of certain markers on ... the laser type. Table adapted from: https://emea.bd.com/panel-design/en/flow-cytometry-fluochrome-brightness-spillover...When two fluorophores bleed into each other, it means that the cytometer could detect a certain photon... parameter in the cytometer's software. It also means we should exclude APC-Cy7 from the rest of our panel...

  3. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...Paramyxoviridae family of viruses, which also includes measles and mumps. Sendai transduces a wide range of cell...endogenous pluripotency genes (i.e. Oct4) are other measures of reprogramming. If a retrovirus was used, then...three germ layers (ectoderm, mesoderm, endoderm). Measuring pluripotency of mouse cells typically involves...injecting tetraploid blastocysts with iPSCs and measuring their ability to direct the development of a mouse...

  4. New Norepinephrine Indicators: nLightG and nLightR

    Type
    Blog Post
    Published
    Dec. 12, 2023, 2:15 p.m.
    ...enhanced response time allows for more precise measurement of norepinephrine uptake throughout the brain...more sensitive than nLightR, both generating a measurable signal after electrical stimulation with a single...single pulse, while nLightR gave a measurable signal not at one pulse, but at the second test point, ten...

  5. An Addgene Co-op: The Intersection of Fun, Friendship, and Work

    Type
    Blog Post
    Published
    June 29, 2023, 7 p.m.
    ...connections with coworkers and doing something meaningful with my career. I can’t pinpoint exactly when...that had never been done at Addgene before. This meant I was responsible for establishing a new report ...present, I thought I would be extremely nervous (I mean I WAS presenting to three department leads, I probably...

  6. Fluorescent Proteins 101: Fluorescent Biosensors

    Type
    Blog Post
    Published
    May 18, 2017, 2:30 p.m.
    ... first need to decide the best way to track or measure the process you’re interested in. Depending on ...examine, i.e. for an enzymatic reaction, you could measure consumption of a substrate OR production of a product...molecule biosensors Addgene has many sensors that measure the concentration of small molecules like arabinose...

  7. Management for Scientists: What Makes a Good Manager Anyway?

    Type
    Blog Post
    Published
    Dec. 9, 2014, 2:22 p.m.
    ...-life balance. It is harder to manage when the measure of work is not just time spent at the desk, but...This requires a focus on deliverables. It does not mean a manager must allow his team to work mostly at ...relationships with clients and colleagues Sometimes this means you have to fire a good performer if they are enough...

  8. Hot Plasmids - June 2020 - Barcoded CRISPR Library, Sparse Cell Labeling, Calcineurin Reporter, and DNA Staining Dye Alternative

    Type
    Blog Post
    Published
    June 2, 2020, 1:15 p.m.
    ...target. Calcineurin reporters allow scientists to measure calcineurin dynamics and can serve as a drug-screening...high throughput screening, calcineurin dynamics measurements, or monitoring in vivo. To overcome these technical...iodine (PI), allowing for calcineurin activity measurements in stressed or aging cell populations. ...

  9. The MTA at Addgene

    Type
    Blog Post
    Published
    July 23, 2024, 2:17 p.m.
    ... a form needs to be signed. But the truth is, meaningful progress depends on things being organized, tracked...forward new discoveries and developments.  MTAs also mean that everyone is on the same page as to how the ...becomes available. Our one-time deposit agreement means we only have to work with one tech transfer office...

  10. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ...increases exponentially with an increase in titer. That means that once you drop below a certain (very high) threshold...concentration to avoid over-diluting, and make sure to remeasure your titer after diluting to confirm. Luke ...Titers: Where do they come from and what do they mean? AAV Vector Quality Control: Going the Extra Mile...

  11. Optimizing Donor DNA for Enhanced CRISPR Genome Editing

    Type
    Blog Post
    Published
    March 24, 2016, 2:30 p.m.
    ...Understanding Cas9 biophysics enhances HDR We first measured Cas9 dissociation rates and discovered that the... of approximately 6 hours. To our surprise, we measured identical dissociation rates for catalytically...replacement rates of approximately 1%. This is by no means a large number, but it was attained without any ...

  12. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ...Sanjana et al., 2014). It is a dual-vector system, meaning it is typically used in conjunction with a second... be carried over to the gRNA lentiviruses. This means that if you received lentiCas9-blast lentivirus ...(Gi)-mCherry from this virus is cre-dependent, meaning that once injected, the DREADD will only be expressed...

  13. GPCRs: How Do They Work and How Do We Study Them?

    Type
    Blog Post
    Published
    Feb. 11, 2021, 2:15 p.m.
    ...modified Tango beta-arrestin recruitment assay to measure receptor activation for more than 300 GPCRs. In...peroxidase-catalyzed proximity labeling to quantifiably measure GPCR agonist response in vivo (Paek et al., 2017...learn about GPCR signaling, researchers in the meantime have developed GPCRs for chemogenetic studies ...

  14. Antibodies 101: Affinity Reagents

    Type
    Blog Post
    Published
    April 14, 2022, 1:15 p.m.
    ...imaging applications, smaller affinity reagents means higher resolution. Many of the below are also small...in an ~50kD protein. Minibodies are bispecific, meaning that each scFv can target a different antigen; ...That is the question… or is it? This post isn’t meant to convert anyone to downsize their affinity reagents...

  15. Channelrhodopsins with improved light sensitivity for minimally-invasive optogenetics

    Type
    Blog Post
    Published
    Nov. 3, 2020, 2:15 p.m.
    ... which often forces scientists to use invasive measures to perform in vivo optogenetic experiments. Intracranial... electrophysiology, a technique that’s used to measure electric current of ion channels in living cells...electrophysiology. All thirty were functional and their measured photocurrent amplitude, wavelength sensitivity...

  16. Antibodies 101: Fab Fragments

    Type
    Blog Post
    Published
    Sept. 5, 2024, 1:15 p.m.
    ...biorender.com     The modularity of antibodies means that it is relatively easy to physically divide ... vivo.     Of course, the lack of an Fc portion means that most of the common anti-IgG secondary antibodies...monovalent Fab’ or Fab fragments. Higher avidity means that F(ab’)2 fragments may have overall stronger...

  17. Enhancing CRISPR Targeting Specificity with eSpCas9, SpCas9-HF1, & HypaCas9

    Type
    Blog Post
    Published
    Dec. 16, 2015, 3:30 p.m.
    ...result in unwanted changes at non-target sites. Means to decrease these so-called “off-target effects”...specificity of Cas9 derived from S. aureus. This means you can likely apply similar rational mutations ..., Chen et al. 2017 used single molecule FRET measurements to determine which portions of Cas9 might be...

  18. CRISPR in the Clinic

    Type
    Blog Post
    Published
    Nov. 2, 2023, 1:15 p.m.
    ...researchers had cured 24 mice of their disease. Meanwhile, Hans Clevers and colleagues applied CRISPR to...success rate in clearing misfolded protein levels as measured in the blood. More information is expected soon...difference, then the treatment will not work in a meaningful way. Delivery, especially for systemic approaches...

  19. Early Career Researcher Toolbox: Finding Relevant Papers

    Type
    Blog Post
    Published
    Jan. 9, 2020, 1:45 p.m.
    ... highly influenced by this paper. Influence is measured by the number of citations and the context of ...You can also create intersects in your feed which means only papers than contain all of your search terms...by papers published each day, week, or month. A measure of the number of times a paper is shared online...

  20. Polymerase Chain Reaction Overview and Applications

    Type
    Blog Post
    Published
    Aug. 10, 2021, 1:15 p.m.
    ... as real time PCR is a PCR technique used for measuring a starting DNA concentration using PCR. qPCR requires...fluorometer feature built into the thermocycler to measure that fluorescent output. With this fluorescent ...droplet is analyzed by a droplet reader, which measures the fluorescence amplitude of each droplet. The...
Showing: 81 - 100 of 461 results