...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...

...highlight a subset of the new plasmids and viral preps in the repository through our hot plasmids articles...

...not just the antibodies that matter when you’re prepping for your experiment - there are a number of outside...

...Addgene's CRISPR resources Request ready-to-use viral preps of select CRISPR lentiviral plasmids Additional...

...research and how they will use plasmids and viral preps from Addgene to further their work. The Association...

...to produce virus? Get the ready-to-use lentiviral prep from the Addgene viral service. CRISPR technology...

... on neuron activity (firing or inhibition). AAV preps from DREADD plasmids allow you to easily express...

... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors References Juettner, J...

...events. Intradermal injections require a bit of extra prep, as the injection site area must be shaved first...

...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...variant (Piatkevich et al., 2023). Check out their preprint for more details. Not only will these mStayGold...doi.org/10.1038/s41592-023-02085-6. PMID: 38036853. Preprint: https://doi.org/10.21203/rs.3.rs-2941917/v1.  ...doi.org/10.1038/s41587-023-02018-w. PMID: 38081970. Preprint: https://doi.org/10.21203/rs.3.rs-2684100/v1.  ...proteins derived from StayGold. ResearchSquare (Preprint). https://doi.org/10.21203/rs.3.rs-3188559/v1....

...chemogenetics in our chemogenetics guide Find viral preps for your research Find chemogenetics plasmids for...

...about your research goals and education, too! Prepping for the interview Many universities offer free...

...Quantify RNA levels Molecular Diagnostics NGS library prep QC ddPCR Determine ultrasensitive and absolute...

...some of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...//doi.org/10.1038/s41477-024-01786-w. (bioRxiv preprint: https://doi.org/10.1101/2024.02.11.579803).  ...

...subset of the new plasmids, antibodies, and viral preps in the repository through our Hot Plasmids articles...doi: 10.1038/s41592-023-01880-5. PMID: 37188954. Preprint available on bioRxiv – doi: 10.1101/2023.03.09.531939...doi: 10.1016/j.cell.2023.05.044. PMID: 37385249. Preprint available on bioRxiv – doi: 10.1101/2023.02.07.527548...doi: 10.1038/s41587-022-01524-7. PMID: 36593408. Preprint on bioRxiv — doi: 10.1101/2021.10.13.463862.  ...

...genome-containing viral particles that are present in an AAV prep. AAV samples are first DNaseI digested to remove...still necessary. To save time and money, I like to miniprep 5 mL of my AAV transfer plasmid cultures and then...pellet for the remainder of the cultures and later maxiprep only the cultures with intact ITRs. I use a spreadsheet...culture supernatant because it’s time consuming to prepare the 40% PEG solution. PEG takes several hours on.... Rather than investing all of this effort in preparing reagents, I choose to only harvest the cell pellet...separate out contaminants from an impure AAV preparation. 15%, 25%, 40%, and 60% iodixanol solutions are...double-check that the iodixanol gradient solutions were prepared correctly. When aliquoting purified and buffer...

...Addgene Browse all AAV vectors Find ready-to-use AAV preps ...STORM imaging, and electron microscopy. Sample preparation for these techniques sometimes makes it more ...retain their ability to be detected after sample preparation better than standard proteins tags like GFP. ...

... Find AAV plasmids Find ready-to-use AAV viral preps Browse all viral vectors ...

... minutes. Additionally, while commercial plasmid prep kits come in discreet sizes (mini, midi, maxi, giga...purification kits. Kit-free alkaline lysis plasmid miniprep Starting material: 2 mL bacterial culture Product...Product: plasmid DNA This kit-free plasmid miniprep protocol from Addgene follows a similar workflow as ...authors estimate that a gravity-based column plasmid maxiprep kit takes 1-1.5 hours to complete, while this ....0007750  Vogelstein, B., & Gillespie, D.A. (1979). Preparative and analytical purification of DNA from agarose...

...vector preparations, we have used quantitative PCR to evaluate the physical titer of our preps. We often... for ddPCR set-up. Prepare the master mix in a separate area from where you prepare your sample dilutions...