We narrowed to 489 results for: RET

...Filonov et al., 2013). FRET biosensors were developed based on miRFP760-miRFP720 FRET pair, including Rac1...indicator (Shemetov et al., 2021). In addition to FRET, intensiometric calcium indicators NIR-GECOs were...optogenetics of Rho GTPases enabled by near-infrared FRET. Nat Chem Biol 14, 591-600 (2018). Shemetov, A.A...

...Evaluating Efficiencies of Dual AAV Approaches for Retinal Targeting. Front Neurosci 11: . https://doi.org...(2013) Effective delivery of large genes to the retina by dual AAV vectors. EMBO Mol Med 6:194–211 . https...truncated proteins are safe and effective in the retina of a mouse model of Stargardt disease. Hum Mol ...first time AAV user Learn about how AAV is used in retinal gene therapy Learn about the parts of an AAV transfer...

...For cloning into and storage of lentiviral and retroviral vectors or cloning or repeated sequences with...cloning and storage of unstable DNA and lentiviral/retroviral vectors. Used by Addgene for pooled library amplification...

...or PNS transduction, cell type specificity, or retrograde transport in neurons. (b) Genetic cargo can be...typically administered in the lateral tail vein or retro-orbital sinus. IT injections are administered in... Chen et al., 2022 PAL2 CNS: cerebrum; neuroretina Macaques N/A Stanton et al., 2023 AAV-MDV1A...could be used to target endothelial cells in the retina and spinal cord (Krolak et al., 2022). Systemic...macaques. It also effectively transduces the neuroretina. PAL2 detargets the liver and has increased DRG...Zheng, Q., Kozareva, V., Beddow, T., Tobey, I. G., Pacouret, S., Chen, A. T., Chan, Y. A., Ryvkin, D., Gu,...

...in real time Proteins involved in endoplasmic reticulum quality control navigate a large and interconnected...factors present unique challenges for study. These secreted signaling proteins regulate vital cell and tissue...subtle changes in co-receptor dynamics, including NanoBRET assays to monitor the binding of fluorescently-labeled..., J., & Hill, S. J. (2021). Use of NanoBiT and NanoBRET to monitor fluorescent VEGF-A binding kinetics...

...remember that some serotypes can be transported retrogradely and/or anterogradely along the axon. Others,...your gene and could lead to toxicity or trouble interpreting your results, while a low transduction efficiency...Puschnik, A. S., Nagamine, C. M., Cheng, F., Qiu, J., Carette, J. E., & Vandenberghe, L. H. (2018). An Alternate...Introduction to AAV Viral Vectors 101: The AAV Retrograde Serotype Viral Vectors 101: Viral Applications...

...to not have any effect on each other, they have discrete effects on the dependent variable, and those variables...others. Each test has different likelihoods of returning false positives/negatives, but they all generally...numerical value relative to quantiles derived from a theoretical distribution. If your data is normally distributed...130 (2012). 10.1097/SMJ.0b013e3182498ad5 Curran-Everett, Douglas. Explorations in statistics: statistical...

...Working on the Eye 9:05-10:16 : Cell Targeting in Retinal Gene Therapies 10:17-14:34 : Progress in Treating...Treating Macular Degeneration and Other Retinopathies 14:39-25:59 : Strategies to Make Gene Therapies More... retina...

...Wilson New serotype AAVrg = AAV retrograde (also called AAV2-retro) Looking for more viral vectors? ...marker, a Flp-dependent channelrhodopsin in the retrograde serotype, and a dTomato under the control of ...

... user, instead, don’t fret; we have backgrounds for you as well! If you use FRET, you might be interested...Fluorescent Proteins blog topic to learn more about FRET and other techniques using FPs! Otherwise, check...

...For example, searching the term "breast cancer" returns 40 screens performed in 26 different breast cancer...and other details relevant to screen result interpretation. The analytical method for score assignment...maintain consistency with published screen interpretations and allows users to query screen data without...

...find this hard so get used to it by making it a concrete goal to say “thank you, job well done” to one ...not the person - intent is usually good. Don’t pretend something went well when it didn’t… but don’t “...compare with your own perception? Tell me your interpretation of my feedback to you. What are We Learning...

...translated into proteins, or, in the case of retroviruses, reverse transcribe their gRNA into DNA, which...replicative ability. The remaining particle, which retains the protein coat (and envelope), has a “gutted”...they’re derived from: AAVs, lentivirus, gamma retrovirus, and adenovirus. And now that you know what viral...

... used as a FRET donor to mCitrine and mOrange or for live cell imaging of fusions and FRET reporters. ...range of applications from traditional imaging to FRET and stochastic single-molecule super resolution ...

...increased yields in adenoviral (Coroadinha, 2006) and retroviral production systems (Shen, 2011). To our knowledge...also improved yields of the AAV2 derivative, rAAV2-retro, but had no effect on AAV5 yields. We further show...Effect of osmotic pressure on the production of retroviral vectors: Enhancement in vector stability." Biotechnology...

...selected to perform the transduction with: either retrovirus, lentivirus, adenovirus, or AAV. Then, your desired...for the transduction step. Viral particles are secreted into the cellular media, making viral collection...integrate into the host genome (lentivirus and retrovirus) while others are non-integrating (adenovirus...

...protein secretion, making harvesting recombinant proteins a cinch. This is because it doesn’t secrete its ...proteins, making the recombinant protein its major secretion. Beyond sheer protein production, K. phaffii is...

... that a longer vent time would be sufficient to return the sample back to the original pH. What about...it back in the freezer? We then tested whether returning the tubes to the -80°C freezer after storage on...the pH changes in our solutions are transient and return to normal once the tube is opened and equilibrium...

...book! Bonus, Saini's following book Superior: The Return of Race Science is a terrifying and equally good...conclusion? In this series, there is always another secret."     - Marcy Patrick, Associate Director of Quality...force them on everyone I know. I am, of course, pretty biased as the founder, but our book club has become...

...team members have opportunities to develop and stretch then they will be happier and more productive in...the person doing the task will have to learn and stretch a little, but be careful not to go too far. Is...Organization Can Help You Develop Leadership Skils Set Concrete Goals with Your Mentor through an Individual Development...