We narrowed to 110 results for: actin

1. Illuminating Epigenetics with A FRET Based Biosensor

   Type

   Blog Post

   Published

   Nov. 19, 2015, 3:30 p.m.

   ...prevents the cellular machinery from physically interacting with the genes in the compacted region and results...

2. xCas9: Engineering a CRISPR Variant with PAM Flexibility

   Type

   Blog Post

   Published

   March 28, 2018, 6:52 p.m.

   ...SaCas9 with an NNGRRN PAM by mutagenizing the PAM-interacting domain. However, these alternative PAM sequences...

3. Truncated gRNAs for Regulating Gene Expression

   Type

   Blog Post

   Published

   Jan. 10, 2017, 3:37 p.m.

   ...Schematic of native (nuclease-competent) Cas9 interacting with either a full-length gRNA (20nt complementarity...

4. What’s in Your Tube?: A Quick Guide to Measuring DNA by Spectrophotometry

   Type

   Blog Post

   Published

   July 1, 2025, 1:15 p.m.

   ...final concentration calculation and potentially impacting downstream experiments. The usual suspects, such...

5. SciComm: Taking Science to Elementary and Middle Schools

   Type

   Blog Post

   Published

   July 9, 2019, 12:52 p.m.

   ...schools have dedicated science teachers. I tried contacting our school district’s science curriculum coordinator...

6. A Career in Grants: How To Become a Grant Professional

   Type

   Blog Post

   Published

   Aug. 20, 2015, 2:30 p.m.

   ...up-to-date on the changing guidelines and regulations impacting grant management, we track grant opportunity deadlines...

7. Fluorescent Proteins 101: Green Fluorescent Protein (GFP)

   Type

   Blog Post

   Published

   May 15, 2014, 3:33 p.m.

   ...elucidating their expression or localization profiles, acting as a biosensor or cell marker, studying protein-protein...

8. Your Lentiviral Plasmid FAQs Answered

   Type

   Blog Post

   Published

   April 23, 2014, 1:08 p.m.

   ... follows: Transfer vectors contain minimal cis-acting HIV components: LTRs, PPT, RRE, and psi packaging...

9. "Hall of Fame" AAV Enhancers from the Allen Institute for Brain Science

   Type

   Blog Post

   Published

   Sept. 9, 2025, 1:15 p.m.

   ...sequences of DNA that regulate gene expression by interacting with proteins such as transcription factors and...

10. Tips and Tricks for Using Golden Gate Modular Cloning (MoClo)

    Type

    Blog Post

    Published

    July 8, 2025, 1:15 p.m.

    ...can check colonies either by PCR, by directly extracting and sequencing the plasmid, or both! We strongly...

11. Transferable Skills Guide: Career Planning Resources

    Type

    Blog Post

    Published

    Nov. 14, 2017, 2 p.m.

    ...information, but not a job! This is good etiquette when contacting someone professionally that you do not know very...

12. Career Insights: Technical Support Specialist

    Type

    Blog Post

    Published

    Nov. 9, 2017, 2 p.m.

    ...and more. Technical support roles also involve interacting with other departments such as research and manufacturing...

13. Supporting Reproducibility with a Connected ELN

    Type

    Blog Post

    Published

    Jan. 17, 2019, 1:38 p.m.

    ...one foot on the other side of the pond through interacting with colleagues in Edinburgh. You can follow ...

14. Hot Plasmids Spring 2024

    Type

    Blog Post

    Published

    May 23, 2024, 1:15 p.m.

    ...CRISPRi screen to identify additional host factors impacting prime editing and found the protein La (a small...

15. CRISPR 101: Which Cas9 Do I Choose for My CRISPR Experiment?

    Type

    Blog Post

    Published

    Oct. 13, 2022, 1:45 p.m.

    ...fusion and a modified gRNA that is capable of interacting with an additional RNA-binding transcriptional...

16. The PAM Requirement and Expanding CRISPR Beyond SpCas9

    Type

    Blog Post

    Published

    Aug. 20, 2020, 2:30 p.m.

    ...7 mutations found in the REC2, REC3, and PAM interacting domains and allows for expanded PAM recognition...

17. An Inside Look into Addgene's Dev Team

    Type

    Blog Post

    Published

    Jan. 23, 2020, 2:45 p.m.

    ...sharing knowledge allows for life to happen without impacting the overall work. Each of our sprints is themed...

18. Plasmids 101: Expressing shRNAs for RNA Interference

    Type

    Blog Post

    Published

    June 17, 2025, 1:15 p.m.

    ...RNA (shRNA) Short interfering RNA (siRNA) Piwi-interacting RNA (piRNA) miRNA and piRNA can be found endogenously...

19. Transferable Skills Guide: Time Management

    Type

    Blog Post

    Published

    Dec. 5, 2017, 1:52 p.m.

    ...internet and all it’s enticements are far too distracting. (As a side note, I’d recommend the book, “Bored...

20. Tips for Getting a Faculty Position

    Type

    Blog Post

    Published

    July 20, 2023, 1:15 p.m.

    ...focus on you and your ideas. Slides will be     distracting. What is the big question? This should be...