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Showing: 81 - 100 of 280 results

  1. Plasmids 101: Knockout/Knock-In Plasmids

    Type
    Blog Post
    Published
    Dec. 1, 2016, 3:30 p.m.
    ...described in the preceding sections are simple ways of knocking out a non-essential gene of interest. However,...Although CRISPR is very good at making knockouts, knocking in large sections of DNA can be more difficult...

  2. Google Forums Round Up: First Impressions of NgAgo

    Type
    Blog Post
    Published
    Aug. 4, 2016, 2:30 p.m.
    ... side, successful indel generation and epitope knockin was achieved by a few respondents suggesting that...inconvenient. Another possible reason for the trickiness of NgAgo is the inability of mature NgAgo protein...

  3. Cloning Mammalian Cells with the Agarose Method

    Type
    Blog Post
    Published
    Sept. 7, 2017, 12:17 p.m.
    ...cloning rings. A picture of a 60 mm dish ready for picking is shown in figure 1.   Find Additional Useful ...firmer 2% agarose gel solution discussed above for picking. Many thanks to our guest blogger Iris Lindberg...

  4. Experimenting with New Careers while in Grad School

    Type
    Blog Post
    Published
    Feb. 25, 2016, 3:30 p.m.
    ...geared toward careers in academia or industry. Luckily, I discovered that there are many ways to get a...of others were key to creating my career path. Luckily, in grad school much of the up front work was done...

  5. AAVs in Retinal Gene Therapy

    Type
    Blog Post
    Published
    July 31, 2018, 12:55 p.m.
    ... this limitation, with limited success so far. Luckily for patients, this gene can be targeted to RPE ...Forever Fix: Gene Therapy and the Boy Who Saved It by Ricki Lewis. ISBN-13: 978-1250015778 7. Yu W and Wu Z....

  7. Finding nucleic acids with SHERLOCK and DETECTR

    Type
    Blog Post
    Published
    April 16, 2020, 1 p.m.
    ... Specific High Sensitivity Enzymatic Reporter UnLOCKING (SHERLOCK and SHERLOCKv2) (Gootenberg et al., ... Specific High Sensitivity Enzymatic Reporter UnLOCKING Cas13 (C2c2) was first identified in 2016 by the...

  8. Plasmids 101: Plasmid Incompatibility

    Type
    Blog Post
    Published
    March 31, 2020, 1:15 p.m.
    ...replication by binding to the origin of replication and blocking replication machinery (del Solar, 1998). As the...occurs for several reasons, including one plasmid blocking replication of another and one plasmid outcompeting...

  9. Plasmids 101: Stringent Regulation of Replication

    Type
    Blog Post
    Published
    June 20, 2024, 1:15 p.m.
    ...different ways: methylating newly synthesized DNA, blocking binding sites, or trading out ADP for ATP to re-activate...mRNA and prevent this pseudoknot from folding, blocking replication. Created with BioRender.com. Plasmids...

  10. Neuronal labeling with Spaghetti Monster

    Type
    Blog Post
    Published
    Aug. 14, 2018, 12:42 p.m.
    ... used for protein localization, isolation, and tracking experiments (Rizzo, 2009). With many different...Davidson, and David W. Piston. "Fluorescent protein tracking and detection: fluorescent protein structure and...

  11. The Challenges of Cell Culture

    Type
    Blog Post
    Published
    May 5, 2016, 2:30 p.m.
    ... good recordkeeping, you need to have a way of tracking how many passages the cells have undergone and... cell line’s age and monitor any changes. Your tracking system could be as simple as a piece of paper ...

  12. Filming Science Videos in the Age of Social Distancing

    Type
    Blog Post
    Published
    May 20, 2021, 1:15 p.m.
    ...we developed a great plan to start consistently knocking out high-quality videos for you all. The next ... me, and to move it out of the way when it was blocking some more important lab work from being done. ...

  14. Transferable Skills Guide: Time Management

    Type
    Blog Post
    Published
    Dec. 5, 2017, 1:52 p.m.
    ...time it will take you to complete the task When picking which tasks to actually do, you should err on the...experience, planning has 3 components: A mechanism for blocking off time for different tasks. I use google calendar...

  15. New Optogenetic Tools for Cytoskeleton and Membrane Control

    Type
    Blog Post
    Published
    Sept. 28, 2023, 1:15 p.m.
    ..., 2023). Rab family proteins support vesicle trafficking and organelle dynamics, but with dozens of family...Optogenetic inhibition of Rab protein vesicle trafficking via CRY2-CIB1 clustering. Image adapted from ...

  16. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...antibody is extremely rare. While many function-blocking integrin antibodies exist, almost all bind outside...to the Springer lab, a highly specific function-blocking antibody, αVβ3.13, may also block function of ...

  17. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    ... GFP or any of the other fluorescent proteins. Luckily there are often solutions for many of the limitations...Chartrand. "Using fluorescent proteins to study mRNA trafficking in living cells." Methods in cell biology 85 ...

  18. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...nucleotides, called dideoxynucleotides (ddNTPs), lacking the 3’ hydroxyl group required for the extension...mouse lines, such as BAC transgenic or traditional knockins/knockouts, a thing of the past. In the sequencing...

  19. Easi-CRISPR: Generating Knock-In and Conditional Mouse Models

    Type
    Blog Post
    Published
    April 5, 2018, 12:42 p.m.
    ...sites for conditional deletion of exon 6 of Syt1, mimicking a previously made mouse model. To avoid disrupting...Easi-CRISPR works best with insertions of 2 kb or less. Luckily, most of the commonly used cassettes, such as fluorescent...

  20. Antibodies 101: Multiplex Immunofluorescence

    Type
    Blog Post
    Published
    May 23, 2023, 1:15 p.m.
    ...binding non-specifically – try optimizing your blocking and washing conditions or consider using a different...concentrations of all the different antibodies, blocking, washing, and sample preparation steps, in order...
Showing: 81 - 100 of 280 results