...fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision, the resulting...the FP-SEC vector using Gibson assembly. This construct will serve as the repair template plasmid to create...

...confirm that you are working with the correct construct, and verify that the plasmid you received matches...and accurately visualize the full size of your construct. Consider buffer and temperature compatibility...

...A>G or T>C mutations in the plant genome. We constructed three versions of the vector (pKABE6.3, pKABE7.9...Agrobacterium-mediated transformation. We also constructed smaller vectors suitable for transient assays...require other base editor platforms. Hua et al. constructed a number of plant base editors replacing the ...

... to infect a cell is dependent on its capsid construction and the cell it’s infecting, getting these particles...not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer...

...permit five-color fluorescent imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc...photobleaching and phototoxicity are a concern, these constructs come in very handy. This approach is especially...

... common E. coli histidine kinase. This hybrid construct senses light, and an obligate chromophore allows...intensity. In addition to these improvements, the constructs have been greatly streamlined; only two plasmids...

...cells.  Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve ...cells are then transfected with several different constructs for the reporter to work and to make the desired...

...experimental work, spend some time outlining the construction of the plasmid and all the steps you will have...used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds of publications...

...suppresses neuronal transmission within minutes. Constructs using the photodimerizer system CRY2-CIB1 to ...immune genes into pXPR_053. They expressed this construct in bone marrow stem cells from Cas9-expressing...

... plasmids episode! New, highly efficient RNAi construct for C. elegans gene knockdown Article contributed...phage display selections. In addition to these constructs, the Sidhu lab has made hundreds of additional...

... Figure 1: (a) Anti-FLAG frankenbody (FB) construction from the complementarity determining region ...and HA tags, you may not even need to clone new constructs to label your favorite protein! While the target...

...experiments. Manufacturers rarely provide the construction plans or CAD (computer aided design) drawings... that allow for on-going creativity with the construction plans. Appropriate open-source licenses for ...

...Cre-lox technology, transgenic mice have been constructed that express Cre under a variety of ubiquitous... many loxP-containing alleles have also been constructed. Additionally, Cre-containing adenovirus (Ad-...

...transposon RNA homology both help direct the fusion construct to the new genomic locus. Image courtesy of Christopher...2025).More than a dozen of these enhancer-AAV constructs are now available as in-stock viral preps using... Look for more of these interneuron-targeting constructs and other serotypes available later this year...

...file. What's available at Addgene? In total, we constructed and tested eight two-input RSMs and three three-input...three-input RSMs. The plasmids used to construct these RSMs are listed in the table below and can be requested...S10A pNR64 pNR166 Fig. S10B pNR64 pNR187 To construct your own two-input or three-input GRSMs, we recommend...

...AID* tagged CRISPR knock-in repair constructs. These repair constructs can be used to integrate the tagged...

...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...

...interest. These plasmids will be most useful for constructing intermolecular FRET probes, where the donor ...may be able to boost FRET efficiency. Before constructing your own FRET probe, try searching PubMed for...

...Figure 1:Schematic diagram of different FingR constructs and experimental results. Image from Bensussen...Bensussen et al., 2020. The FingR constructs target the synaptic scaffolding proteins PSD95 and gephyrin...

...library delivers 2 randomly paired guides per construct and allows a reduced scale in screens without ...serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1...