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We narrowed to 245 results for: construct

Showing: 81 - 100 of 245 results

  2. Even more elegant: Single injection CRISPR/Cas9 in C. elegans

    Type
    Blog Post
    Published
    July 7, 2015, 3:36 p.m.
    ...fluorescent protein (FP) and a 3xFLAG tag (see construct schematic below.) After self-excision, the resulting...the FP-SEC vector using Gibson assembly. This construct will serve as the repair template plasmid to create...

  4. CRISPR-mediated Plant Base Editors

    Type
    Blog Post
    Published
    Jan. 3, 2019, 1:35 p.m.
    ...A>G or T>C mutations in the plant genome. We constructed three versions of the vector (pKABE6.3, pKABE7.9...Agrobacterium-mediated transformation. We also constructed smaller vectors suitable for transient assays...require other base editor platforms. Hua et al. constructed a number of plant base editors replacing the ...

  5. Luminescent Imaging with Nano-lanterns

    Type
    Blog Post
    Published
    May 25, 2017, 2:30 p.m.
    ...permit five-color fluorescent imaging! These eNL constructs use the brightest characterized luciferase, NanoLuc...photobleaching and phototoxicity are a concern, these constructs come in very handy. This approach is especially...

  6. The Importance of High Titer for AAV Transductions

    Type
    Blog Post
    Published
    March 1, 2018, 2:11 p.m.
    ... to infect a cell is dependent on its capsid construction and the cell it’s infecting, getting these particles...not need to be as high when delivering receptor constructs with AAV. In the case of channelrhodopsins, fewer...

  7. Synthetic Photobiology: Optogenetics for E. coli

    Type
    Blog Post
    Published
    Sept. 8, 2015, 2:30 p.m.
    ... common E. coli histidine kinase. This hybrid construct senses light, and an obligate chromophore allows...intensity. In addition to these improvements, the constructs have been greatly streamlined; only two plasmids...

  8. Troubleshooting Your Plasmid Cloning Experiment

    Type
    Blog Post
    Published
    Sept. 24, 2019, 12:55 p.m.
    ...experimental work, spend some time outlining the construction of the plasmid and all the steps you will have...used for cloning of more than 10,000 DNA custom constructs. His work was acknowledged in hundreds of publications...

  10. Fluorescent CRISPR Reporters: SRIRACCHA and GEmCherry2

    Type
    Blog Post
    Published
    May 26, 2020, 1:15 p.m.
    ...cells.  Figure 1: The SRIRACCHA reporter construct. Image from Wen et al., 2017. Once you’ve ...cells are then transfected with several different constructs for the reporter to work and to make the desired...

  11. Sharing is Caring: But How to Distribute Open Hardware?

    Type
    Blog Post
    Published
    Feb. 28, 2023, 2:15 p.m.
    ...experiments. Manufacturers rarely provide the construction plans or CAD (computer aided design) drawings... that allow for on-going creativity with the construction plans. Appropriate open-source licenses for ...

  12. Hot Plasmids - February 2022

    Type
    Blog Post
    Published
    Feb. 22, 2022, 2:15 p.m.
    ... Figure 1: (a) Anti-FLAG frankenbody (FB) construction from the complementarity determining region ...and HA tags, you may not even need to clone new constructs to label your favorite protein! While the target...

  14. Plasmids 101: Cre-lox

    Type
    Blog Post
    Published
    Jan. 13, 2015, 3:47 p.m.
    ...Cre-lox technology, transgenic mice have been constructed that express Cre under a variety of ubiquitous... many loxP-containing alleles have also been constructed. Additionally, Cre-containing adenovirus (Ad-...

  15. Hot Plasmids: Spring 2025

    Type
    Blog Post
    Published
    June 3, 2025, 1:15 p.m.
    ...transposon RNA homology both help direct the fusion construct to the new genomic locus. Image courtesy of Christopher...2025).More than a dozen of these enhancer-AAV constructs are now available as in-stock viral preps using... Look for more of these interneuron-targeting constructs and other serotypes available later this year...

  16. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...file. What's available at Addgene? In total, we constructed and tested eight two-input RSMs and three three-input...three-input RSMs. The plasmids used to construct these RSMs are listed in the table below and can be requested...S10A pNR64 pNR166 Fig. S10B pNR64 pNR187 To construct your own two-input or three-input GRSMs, we recommend...

  17. Hot Plasmids and Viral Preps - March 2021

    Type
    Blog Post
    Published
    March 23, 2021, 1:15 p.m.
    ...AID* tagged CRISPR knock-in repair constructs. These repair constructs can be used to integrate the tagged...

  18. Important Considerations When Using AAVs

    Type
    Blog Post
    Published
    June 13, 2017, 2:30 p.m.
    ...AAV vectors to deliver chemogenetic/optogenetic constructs into our cells of interest. Although they have...time until expression, it is possible to use AAV constructs with self-complementing structure (scAAV) (7)... would result in cell death. With several new constructs becoming available, AAVs could be much better...

  19. Fluorescent Proteins 101: Introduction to FRET

    Type
    Blog Post
    Published
    June 27, 2017, 1:03 p.m.
    ...interest. These plasmids will be most useful for constructing intermolecular FRET probes, where the donor ...may be able to boost FRET efficiency. Before constructing your own FRET probe, try searching PubMed for...

  20. Hot Plasmids and Viral Preps - January 2021

    Type
    Blog Post
    Published
    Jan. 5, 2021, 2:15 p.m.
    ...library delivers 2 randomly paired guides per construct and allows a reduced scale in screens without ...serotypes are now available for Gordon Fishell Lab constructs targeting GABA neurons. Serotypes include AAV1...
Showing: 81 - 100 of 245 results