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We narrowed to 872 results for: ica;

Showing: 81 - 100 of 872 results

  1. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ... receptors which play a critical role in development and are often implicated in disease progression. ...inability to replicate the spatio-temporal precision with which signaling events occur in a physiological setting...brighter mutant S65T-GFP, and the subsequent biochemical characterization, has enabled scientists to engineer...observations: Rapamycin is a membrane-permeable pharmacological agent that can rapidly induction heterodimerization...flexible cloning process provides improvements to classical cloning methods, particularly for complex transgenesis...enabled the group to perform independent two-color optical excitation of neurons. The paper describes five...setting. Genetic techniques typically rely on overexpression or knock-down, where signaling dynamics are...

  2. Viral Vectors 101: Systemic Capsids

    Type
    Blog Post
    Published
    Oct. 12, 2023, 1:15 p.m.
    ...rather than being geographically constrained to the brain. In cases where it’s critical to minimize or eliminate...recommended for all systemic capsid applications. For example, AAV-PHP.eB is typically administered at a total dose...your specific application and animal model.  Other model organisms Tropism and efficacy can vary depending... 2023 * indicates viral preps available. Mechanisms of crossing the BBB are indicated in parentheses...crossing into the brain. While helpful from a biological standpoint, it makes delivering AAVs to the brain...throughout the organism to target large or diffuse biological structures. Of primary interest here are systemic...suitability of a systemic capsid for an individual application is dependent on a number of factors, including...

  3. 27 Hot Plasmids from 2016

    Type
    Blog Post
    Published
    Dec. 22, 2016, 3:03 p.m.
    ...FAIRE-seq, and CHIP-seq), genome-wide identification and quantification of enhancer activity have remained...Identifying new ligands for orphan GPCRs is of significant medical interest, however efforts to do so have been...GMAP-compatible vectors for a wide variety of biological applications. First, GMAP-compatible backbones for lentivirus...mutant library generation in several biotechnologically and clinically relevant bacterial species. pORTMAGE...backgrounds can lead to disparate physiological effects and complicate any metabolic study. The Ralser ...brief articles highlight the main features and applications of a partiular plasmid or set of plasmids. We...Wu Lab library can be found in the associated publication. Ma, et al. Cell Rep. 2015. PubMed PMID: 26190106...

  4. GCE4All: Making Genetic Code Expansion Accessible

    Type
    Blog Post
    Published
    June 15, 2023, 1:15 p.m.
    ...protein site-specifically to alter its function, mimic a biologically relevant modification, or add in ...function relationships or, in more practical applications, allow a protein to be more effectively used in ...presents a significant barrier for those wishing to use it as a tool. In short, while the technical skills...proteins, you’re probably quite interested in the canonical 20 amino acids. But in your quest to learn more...code expansion (GCE): techniques in which non-canonical amino acids (ncAAs, also sometimes called unnatural...in fluorescent or chemically reactive side chains, all of which can help determine structure and function...was developed into an E. coli system, with the noncanonical amino acid in the media and the tRNAs and synthetase...

  5. Fluorescent Proteins 101: When GFP lets you down

    Type
    Blog Post
    Published
    Aug. 23, 2018, 12:05 p.m.
    .... "A genetically encoded fluorescent probe in mammalian cells." Journal of the American Chemical Society...). GFP is the most popular, most widely used genetically encoded fluorescent probe. Several factors contribute...probe, it is the first choice when selecting a genetically encoded fluorescent tag. There are, however, ... proteins.Therefore, GFP is not suitable for applications that require anaerobic conditions. In experiments...of these strategies works best for a specific biological system under anaerobic conditions needs to be...adding GFP may alter diffusion rates. Smaller genetically encoded probes are based on cofactor binding ...tags are peptides that are engineered to bind specifically and with high affinity to fluorophores. Examples...

  6. Summer SciComm: Preprints

    Type
    Blog Post
    Published
    Aug. 16, 2022, 1:15 p.m.
    ...or feedback. One of the most significant shifts in scientific communication is the rising use of preprints...arXiv.org), but have been only adopted by biological and biomedical scientists (see: bioRxiv.org or medRxiv.org...the most important part of your research is communicating the results of the work. Yes, those control ...any impact! There are lots of ways scientists communicate with each other: from formal mechanisms like ...poster or talk, or submitting patent or grant applications; to informal settings like lab meetings, social...publishing in a journal.  Accelerating scientific communication Preprints have been common in fields like math...increasingly growing time from submission to publication. It takes many months, often more than a year...

  7. Troubleshooting and Optimizing a Western Blot

    Type
    Blog Post
    Published
    Sept. 17, 2024, 1:15 p.m.
    ...people will simply load an extra ladder or technical replicate (ie, leftover sample) to fill the relevant...background, your issue is likely technical. White circles, for example, could indicate that you had air trapped...Sensitivity in Western Blotting. Journal of Pharmacological and Toxicological Methods, 64, 233–237. https://doi....to get right. If you’ve thoughtfully done the technical design of your blot, but are still finding yourself... be done at 4 °C or on ice, whichever is more practical. If your lysing method is not resulting in your...nuclear- or DNA-binding proteins, you may need to sonicate your lysates in order to release the proteins ...you may want to consult Tables 1 and 2 in our Technical Design of a Western Blot post to make sure you...

  8. Viral Vectors 101: Preparing Pooled Libraries

    Type
    Blog Post
    Published
    July 13, 2023, 1:15 p.m.
    ...you.  Starting from plasmids requires amplification, verification, and lentiviral production steps before...the same proportion after amplification as they were prior to amplification. And as anyone who has worked...library can drastically impact what conditions it needs to properly amplify.  Amplification protocols therefore... prep library. Addgene’s pooled libraries are typically DNA preps, but we do distribute several of our...process for using pooled lentiviral libraries. A) Amplification, validation, and lentiviral generation of a ...pooled library is transformed into E. coli for amplification. Transformed E. coli are allowed to grow on ...into HEK cells allows production of lentivirus. Purification of this lentivirus results in the pooled library...

  9. pCXLE toolkit: Efficient episomal plasmid-based method to reprogram peripheral blood cells to iPSCs

    Type
    Blog Post
    Published
    Dec. 14, 2017, 2:08 p.m.
    ...Diecke, S., et al., Recent technological updates and clinical applications of induced pluripotent stem...opened up vast possibilities for the clinical and therapeutic applications of iPSCs. The generation of human... patients, specifically children and patients with skin disorders for research and clinical trials, as...derivation of clinical grade hiPSCs, reprogramming methods that do not involve modification of the original... OriP and induces amplification of the episomes concurrently with DNA amplification in the host cell. ...system thus provides a practical way to generate transgene-free, virus-free clinical grade hiPSCs from easily...therapy trials and remains a safety concern in the clinical field [4]. Three major reprogramming methods are...

  10. Scientific Reproducibility - Focusing on Solutions at the Minisymposium on Reproducibility

    Type
    Blog Post
    Published
    May 18, 2018, 7:32 p.m.
    ...experiments with too few replicates leading to suspect statistical significance (you can read more about...someone has replicated it precisely. You can find a great discussion of different types of replication in Nosek...formulating new biological concepts. However, rates of reproducibility for preclinical studies, as reported...perfect story for a “high impact” publication can lead to the publication of rare flukes showing positive...more practical) is pre-registration and review of experimental design as part of the publication process...on solutions to reproducibility issues in the biological sciences and featured speakers from academia,...Overview - Jeffrey S. Flier, Researcher at Harvard Medical School, former dean of the faculty of Medicine ...

  11. 6 Tips for Grant Writing

    Type
    Blog Post
    Published
    Oct. 18, 2016, 2:30 p.m.
    ...Series 4. Become a science communicator More and more fellowship applications are asking for a 200-300 ...good quality application. Even following this advice, only about 50% of my grant applications have been ...your grant application to your peers and collaborators can also help you get some critical insight into...do need to be able to research and write your application, then give it to someone else to read and / or...it). It’s easy for reviewers to spot when an application is rushed (whether the idea isn’t properly researched...researched / developed or the application is full of typos) and these are unlikely to get past the initial...someone else in the department) to read over your application and give you feedback. They’ve most likely been...

  12. Using AAV for Neuronal Tracing

    Type
    Blog Post
    Published
    Aug. 9, 2018, 1:04 p.m.
    ...production and purification that yield high titers (see also AAV protocols for production, purification and titration...higher specificity and reduced technical variability compared to chemical tracers. rAAV2-retro also has...ability to spread trans-synaptically on to subsequent neurons. AAV vectors are replication incompetent and cannot...intracerebral application, which is more invasive and has a much higher risk of complications. References...between different brain regions. For example, communication between the hippocampus and the prefrontal cortex...memory, a special type of memory which includes autobiographical events (see Jin & Maren, 2015). Directional...intracellularly spread away from the site of application and aid in visualization of the morphology of...

  13. A Guide to Getting Started in Undergrad Research

    Type
    Blog Post
    Published
    June 8, 2021, 1:15 p.m.
    ...research? I grew up knowing little to no biological or biomedical scientists and had no idea what the daily... global problems. Biomedical research, for instance, contributes heavily to medical practice, and understanding...comes your way. Think critically about whether it’s something you want to dedicate your time and energy...These conferences have a lot of programming specifically dedicated for career advancement, and for students...entire page dedicated to different career options from science policy to science communication and much ...the Daily Psychological Experiences of First-Generation College Students. Social Psychological and Personality...hazardous liquids. Working in a lab is nothing like a typical part-time or 9-to-5 job. I had to ask to find out...

  14. 9 tips for a successful postdoctoral experience

    Type
    Blog Post
    Published
    Oct. 9, 2018, 12:21 p.m.
    ...get there and more practically, when do you need to be there? A typical biomedical postdoc fellowship ... findings that fail to replicate. Plan experiments logically and strategically to increase your rate of...theJanelia Research Campus of the Howard Hughes Medical Institute. Today's postdoctoral fellows (PFs) face...need? Multiple first or corresponding author publications (published, not in preparation) A history of...submitted manuscript in a high-profile journal typically takes 6-12 months depending on the revisions and... to be ready to write by Year 3.5. Thus, it is critical to get an experimental system up and running very...The other items will follow. If you get more publications, you will be more competitive for fellowships...

  15. Antibodies 101: Affinity Tags

    Type
    Blog Post
    Published
    Sept. 19, 2023, 1 p.m.
    ...Waugh, 2002). The tag can also be proteolytically cleaved after purification, so as not to perturb protein...for downstream applications. Tag cleavage must be performed after primary purification and requires a ...thoughtful planning, affinity tags can make protein purification a cinch. Types of affinity tags, the proteins... Affinity tags are used primarily as protein purification tools (and can have secondary functions as detection...single protein is expressed, with the intent of purification, it will be expressed along with all other cellular...available antibodies, resins, beads, and other purification aids readily available. Choosing the best tag...tag to remain on your protein for downstream applications Below, we describe a few of the most common...

  16. Antibodies 101: The Basics of Western Blotting

    Type
    Blog Post
    Published
    March 8, 2021, 2:41 p.m.
    ...the sandwich and typically takes less than an hour. However large proteins typically do not transfer as...validated applications, pay close attention to the controls listed on the vendor’s website. Typically, the ...downregulated in a system, detect post-translational modifications, quantify protein levels relative to standards... boiling in the presence of a reducing agent, typically containing thiols, to cleave disulfide bonds. ...gel on top of the resolving gel. A stacking gel typically has a different ionic strength and lower pH and...are “sticky” and have the potential to bind nonspecifically to the antibodies used for staining. To address...search, first pay attention to the validated applications listed on the vendor’s website and choose an...

  17. Plasmids 101: NGS Quality Control for Pooled Libraries

    Type
    Blog Post
    Published
    Oct. 26, 2017, 1:59 p.m.
    ...would be a good time to talk about the amplification and verification processes we use to ensure high-quality...your own experiments. Library amplification 101 Library amplification seems straightforward: E. coli ...represented. Typically, Addgene uses electrocompetent Stbl4 cells when performing amplifications, and we use...transformed with the library DNA, they grow and replicate, and then their DNA is harvested, much like one...library has its own depositor transformation and amplification protocol, but the overall workflow is similar...ll need to remember when undertaking library amplification and sequencing. Maintain representation of the...ensure a good transformation efficiency. For a typical library, Addgene usually does four to eight electroporation...

  18. Plasmids 101: Protein Expression

    Type
    Blog Post
    Published
    June 7, 2018, 1:17 p.m.
    ... expression for experimental, biotechnological, and medical applications. Researches can visualize proteins...of complex post-translational modifications. Extraction and purification of recombinant proteins from ...of proteins including antibodies and pharmaceuticals, specifically interleukins. Yeast expression systems...downstream applications. Cell-free expression systems however, are expensive and very technically challenging...modified via an array of post-translational modifications including protein folding, formation of disulfide...mRNA and the addition of post-translational modifications Learn more molecular biology in our Plasmids...expression systems used for protein production and purification. These include mammalian, insect, bacterial,...

  19. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...colleagues have developed a new genetically-encoded voltage indicator (GEVI) called JEDI-2P (Liu et al...Verkhusha’s lab has generated a new genetically encoded calcium indicator using small monomeric NIR fluorescent...Tuning the Sensitivity of Genetically Encoded Fluorescent Potassium Indicators through Structure-Guided...colleagues present an improved genetically encoded temperature indicator B-gTEMP designed to meet those... B-gTEMP itself is a significant improvement over previous temperature indicators, it also demonstrates...doi: 10.1038/s41592-022-01597-x. New glutamate indicator iGluSnFR3 Improving on the glutamate sensor iGluSnFR...They generated variants optimized for different applications (v82 has higher affinity for glutamate but slower...

  20. Plasmids 101: Biotinylation

    Type
    Blog Post
    Published
    Nov. 15, 2018, 1:50 p.m.
    ...or macromolecule. Biotin can be added enzymatically or chemically. Enzymatic biotinylation occurs naturally...molecular biology applications for biotin and avidin. A short list of some other common applications and links...downstream application. Biotin labelled oligonucleotides can be used in a multitude of applications including...post-translational modification of exceptional specificity.” Trends in Biochemical Sciences, 24.9 (1999b... M. “Biotin: The forgotten vitamin.” American Journal of Clinical Nutrition 75.2 (2002): 179–180. PubMed...dye-labeled molecules on surfaces.” Journal of the American Chemical Society 125.27 (2003): 8074–8075. PubMed PMID...this highly specific post-translational protein modification. Most organisms have less than five enzymes ...
Showing: 81 - 100 of 872 results