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We narrowed to 256 results for: idua

Showing: 81 - 100 of 256 results

  1. An Integrin Antibody Toolkit from IPI

    Type
    Blog Post
    Published
    March 28, 2024, 1:15 p.m.
    ...needed specific tools to interpret the roles of individual integrins. The Ha lab — in collaboration with...investigators inhibited other RGD-binding integrins, individually and in combination, showing that among the three...

  2. Deep Mutational Scanning with One Pot Saturation Mutagenesis

    Type
    Blog Post
    Published
    Feb. 22, 2017, 3:30 p.m.
    ...a single mutagenic primer pair to generate an individual mutant, this technique uses mutagenic primers...The PCR product is digested with DpnI to remove residual starting template. After transformation and harvest...

  3. Evolution of Lab Techniques

    Type
    Blog Post
    Published
    June 21, 2016, 2:30 p.m.
    ...southern blotting are conducted on a sample from an individual without sickle cell anemia, two DNA fragments...sequencing could move from sequencing cores to individual labs and even out into the field. Current versions...

  4. Treating Muscular Dystrophy with CRISPR Gene Editing

    Type
    Blog Post
    Published
    Jan. 26, 2016, 3:30 p.m.
    ... function. Without functioning dystrophin, an individual experiences progressive muscle wasting leading..., but at least partially functional protein. Individuals with these mutations are often asymptomatic, ...

  6. A Control for All Seasons

    Type
    Blog Post
    Published
    Oct. 5, 2023, 1:15 p.m.
    ... are likely just natural differences between individuals. Recommended controls for antibody-based experiments...include a control wherein each primary antibody is individually omitted from the staining but all secondary ...

  8. Recombinase-based State Machines Enable Order-dependent Logic in vivo

    Type
    Blog Post
    Published
    July 28, 2016, 2:30 p.m.
    ...sequence the register. In our paper (1), we allowed individual bacterial cells to form colonies on a plate and...annealing temperature, and ~50% GC content) so that individual parts and/or strings of parts may be PCR-ed out...

  9. Plasmids 101: Restriction Cloning

    Type
    Blog Post
    Published
    Feb. 18, 2016, 3:42 p.m.
    ...different locations and can be cut out of the gel individually. There are a variety of ways to visualize the...your ligation has worked, you will need to pick individual bacterial colonies and check them for successful...

  10. How to Deposit Your Plasmids with Addgene

    Type
    Blog Post
    Published
    Feb. 27, 2024, 2:15 p.m.
    ...multiple inserts, enter details for each insert individually.   Figure 9: Add cloning information for the...for your first plasmid and can be used on each individual page (Gene and Insert, Cloning Information, Growth...

  11. Deep Dive: qPCR

    Type
    Blog Post
    Published
    Sept. 29, 2022, 1:15 p.m.
    ...target in the reaction should be compared to an individually run sample when developing the protocol and ...and optimized until the multiplexed and individual values match across the board. If the samples or sample...

  12. Delivery Methods for Generating iPSCs

    Type
    Blog Post
    Published
    April 17, 2018, 1:37 p.m.
    ...Insertional mutagenesis, transgene reactivation, residual expression Lentivirus Integrating High Low ...Stable Genome integration Insertional mutagenesis, residual expression piggyBac Excisible Medium Medium...

  13. Technical Design of a Western Blot

    Type
    Blog Post
    Published
    Aug. 22, 2024, 1:15 p.m.
    ...the manufacturer, each rAb in a mixture can be individually validated. ‌rAbs are not as commonly available...fast (often <10 minutes) and simple, utilizing individually packaged membrane ‘sandwiches’. They work well...

  14. 15 Hot Plasmids from 2017

    Type
    Blog Post
    Published
    Jan. 9, 2018, 3:02 p.m.
    ...fusion protein retained the PAM properties of the individual Cas9. Kim et al. also mutagenized the cytidine...proposed to be essential in B. subtilis 168. Each individual strain includes a xylose-inducible dCas9 paired...

  15. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...and more. It's this diverse group of talented individuals that makes Addgene — well, Addgene. We're so ...

  17. Congratulations, Deck The Lab winners!

    Type
    Blog Post
    Published
    Jan. 14, 2021, 1:30 p.m.
    ...place, the BTC Institute decorated with dozens of individual virus snowflakes, since snowflakes and viruses...

  20. 500,000 Plasmids Shipped and Counting!

    Type
    Blog Post
    Published
    March 26, 2015, 3:24 p.m.
    ... the daily workings in Addgene's lab. We ship individual plasmids as transformed bacteria in stab culture...
Showing: 81 - 100 of 256 results