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We narrowed to 486 results for: mme

Showing: 81 - 100 of 486 results

  1. How Dry Ice Affects Viral Vector Sample pH & How to Avoid It

    Type
    Blog Post
    Published
    June 20, 2017, 2:30 p.m.
    ...the CO2 goes out. Conclusions and handling recommendations Not all O-rings function optimally at -80°C...results and the results reported elsewhere, we recommend that once you receive your AAV from Addgene, you...to thaw. However, if this is not possible, we recommend both venting the tube for at least 30 seconds ...

  2. Plasmids 101: Gram Negative and Positive Bacteria

    Type
    Blog Post
    Published
    Aug. 2, 2022, 1:15 p.m.
    ...onto the smear until the alcohol runs clear and immediately rinse. Flood the smear with the safranin counterstain...blot gently to dry, and then view under an oil immersion using a light microscope. Gram positive bacteria...a lab.   Anna Kamens is a science communication summer intern at Addgene and a rising third-year at Old...

  3. Predicting Adverse Reactions to Monoclonal Antibody Drugs

    Type
    Blog Post
    Published
    Feb. 13, 2024, 2:15 p.m.
    ...are multiple algorithms, both open-access and commercial, that are commonly used for the prediction of...common open-access tools, while EpiMatrix is a commercially available algorithm to evaluate the immunogenicity...doi.org/10.1016/B978-0-12-417144-2.00027-5van Brummelen, E. M., Ros, W., Wolbink, G., Beijnen, J. H., ...

  4. A Deep Dive into BioBuilder

    Type
    Blog Post
    Published
    Oct. 11, 2017, 1:44 p.m.
    ...economically disadvantaged students for paid summer internships. A summer teacher professional development program...with both of these resources road tested over the summer with dozens of teachers around the country. How...

  5. Antibodies 101: Buffers, Storage, and Conjugates

    Type
    Blog Post
    Published
    Feb. 1, 2022, 2:15 p.m.
    ...conjugates, blocking the conjugation reaction. Many commercial antibodies come with sodium azide already included...reaction is done, through a variety of methods or commercial kits.  However, antibodies conjugated to horseradish...use it for your ELISA and Western, I’d highly recommend sticking with what works.  To balance this out...

  6. How Addgene Creates Content

    Type
    Blog Post
    Published
    Nov. 17, 2022, 2:15 p.m.
    ...blot out quickly." Final design (right). Immediate and immense regret of the graphic designer not shown...microscopy and flow cytometry experts for the panel recommendations. A few discussions in the review process led...

  7. Top Requested Lentivirus and AAV of 2016

    Type
    Blog Post
    Published
    Jan. 6, 2017, 3:56 p.m.
    ... Roth Lab recommends verifying cre expression prior to use. The lab additionally recommends using an anti-mCherry...effect doesn’t confound your experimental data, we recommend generating a monoclonal Cas9-expression line prior...

  8. REPLACR Mutagenesis: Replacing In Vitro Recombination Methods

    Type
    Blog Post
    Published
    Feb. 10, 2016, 3:30 p.m.
    ...but plasmid-based SDM has lagged behind. While commercial kits are available for making small point mutations...plasmid, which could introduce mutations. Using commercially available high-fidelity polymerases such as ...as KOD and Phusion, which are recommended for plasmids up to 25 kb, most cloners should be fine, especially...

  9. Dos & Don'ts When Publishing a Scientific Manuscript

    Type
    Blog Post
    Published
    May 22, 2014, 4:02 p.m.
    ...reviewer’s comments without explaining why. If it looks like you have ignored their comments, they will.... In addition, authors and readers are able to comment on the referee reports. If the reviewers suggest...

  10. Antibodies 101: Conjugation

    Type
    Blog Post
    Published
    March 26, 2024, 1:15 p.m.
    ...reactions. After all, there is an abundance of commercially available conjugated antibodies that are ready...talking about this? Even though there are so many commercially conjugated antibodies available, that never ...antigen recognition and affinity. Hopefully, a commercially available conjugated antibody will have been...

  12. Seven Tips for Using LinkedIn as a Scientist

    Type
    Blog Post
    Published
    Oct. 1, 2019, 1:21 p.m.
    ...connecting. You can see what they post and occasionally comment on their posts as appropriate, possibly giving ...Keep it professional at all times Do not post or comment on anything that is not relevant to business or...inappropriate content see this article. However, do comment on other people’s news and updates. Share your ...

  13. 5 Steps to Writing Grant Proposals for Grad Students

    Type
    Blog Post
    Published
    Feb. 11, 2014, 12:13 p.m.
    ...consider the reviewer's comments. Whether the proposal is funded or not, the comments help gauge room for ...include for the next application. Although some comments may make it clear that the reviewer misunderstood...

  14. Antibodies 101: Stripping and Reprobing Western Blots

    Type
    Blog Post
    Published
    Aug. 1, 2024, 1:15 p.m.
    ...trying to strip and reprobe. In this case, we recommend using mild stripping solution (see below) to minimize...Run your gel and membrane transfer as normal. Immediately after transfer, dry the membrane to maximize ...retention. Following this step, most PVDF membranes recommend re-wetting with methanol before rinsing with wash...

  15. Corralling and Cornering the Goldilocks Antibody

    Type
    Blog Post
    Published
    Aug. 9, 2022, 1:15 p.m.
    ...scientists, I have spent hours sifting through comments and papers to find the most reliable (and cost-effective... crisis” came to light as antibodies became commercialized, revealing the unreliability of antibodies....antibody crisis states that up to 50% of all commercially available antibodies are unreliable (Baker, ...

  16. Viral Vectors 101: Chemogenetics

    Type
    Blog Post
    Published
    July 11, 2023, 1:15 p.m.
    ...takes on the order of minutes. Studies looking at immediate effects of specific neuron firing are better equipped...protein-coupled receptors (GPCRs), that have been reprogrammed to respond only to inert, nonnative ligands....Mouravlev A, Carpenter JC, Snowball A, Knauss S, von Schimmelmann M, During MJ, Lignani G, Schorge S, Young D,...

  17. Design Tips for Prime Editing

    Type
    Blog Post
    Published
    Jan. 23, 2025, 2:15 p.m.
    ...software tools they recommend — feel free to share your favorite software in the comments below! References...2019). When possible, the PE3b/PE5b approach is recommended over the PE3/PE5 approach. Figure 7: ...

  18. Overcoming the Challenges of Lentiviral Production

    Type
    Blog Post
    Published
    Aug. 7, 2018, 12:31 p.m.
    ...optimization. Before beginning an experiment, it is recommended that you try a few different ratios of transfer...will need to be optimized prior to starting. We recommend calculating the ratio of the surface area of the... target cell line. To overcome this issue, we recommend using a range of doses of lentivirus when transducing...

  19. Lab Automation at Addgene

    Type
    Blog Post
    Published
    March 22, 2018, 1:28 p.m.
    ...you can get one. As a starting point, I would recommend conferences like Lab Robotic Interest Group (LRIG...machine fits on a table top and can be easily programmed for a variety of lab needs. Addgene uses it to... tips and tricks, be sure to share them in the comments section.   Additional Resources on the Addgene...

  20. 5 Tips for Troubleshooting Viral Transductions

    Type
    Blog Post
    Published
    Aug. 11, 2016, 2:23 p.m.
    ...the virus immediately upon harvesting. Interestingly, freeze-thawing host cells immediately prior to their...of cell culture medium on the packaging cells immediately following transfection. In other words, when ...
Showing: 81 - 100 of 486 results