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  2. Data Freedom: The Expansion of Data Sharing in Research Publications

    Type
    Blog Post
    Published
    Aug. 5, 2014, 6:51 p.m.
    ...strong incentives to publish such as precedence, recognition and “productivity”, so this is unlikely. Usually...important, what data should be included? We should recognize that storing data in a sharable form is indeed...

  3. Antibodies 101: Introduction to Immunofluorescence

    Type
    Blog Post
    Published
    Nov. 16, 2021, 2:15 p.m.
    ...considerations are to make sure your secondary 1) will recognize the species of your primary antibody and 2) is...primary antibody, first, remember that antibodies recognize very specific 3-dimensional epitopes. Different...

  4. Hot Biosensors 2022: Year-End Roundup

    Type
    Blog Post
    Published
    Dec. 6, 2022, 2:15 p.m.
    ...regions of any G-protein-coupled receptor (GPCR) recognizing a specific ligand (Ino et al. 2022). The oxytocin...more than 20 other MTRIA sensors based on GPCRs recognizing various neurotransmitters, neuropeptides and ...

  5. CRISPR 101: Validating Your Genome Edit

    Type
    Blog Post
    Published
    Nov. 3, 2022, 12:15 p.m.
    ...existing restriction enzyme recognition sequence or generate a new recognition sequence. In these cases,...

  6. CRISPR 101: Engineering the Plant Genome Using CRISPR/Cas9

    Type
    Blog Post
    Published
    Oct. 11, 2016, 2:30 p.m.
    ...plasmid between the T-DNA borders (yellow) are recognized by the Vir genes on the helper plasmid and used...with chemical cargoes in plants enabled by a biorecognition motif. Nat Commun 11: . https://doi.org/10.1038...

  7. Site Directed Mutagenesis by PCR

    Type
    Blog Post
    Published
    Aug. 2, 2016, 2:30 p.m.
    ...that is methylated at the adenosine of the GATC recognition site. Transformation: After the PCR reaction,...can be achieved using a restriction enzyme that recognizes a unique site in the coding region (preferably...

  9. With an Eye Towards the Future, We Look Back at the March for Science

    Type
    Blog Post
    Published
    Oct. 24, 2017, 1:54 p.m.
    ...courage to make mistakes and 2) the integrity to recognize these mistakes quickly. However, many scientists... I was proud to be a part of a community that recognizes doing science (and doing it ethically) runs hand...

  10. 22 Hot Plasmid Technologies from 2014

    Type
    Blog Post
    Published
    Jan. 6, 2015, 5:21 p.m.
    ...ability of Type IIS enzymes to cut outside their recognition site, and permits DNA fragments with compatible...assembled. Scientists can engineer unique enzyme recognition sites that flank a DNA module in an inverse orientation...

  12. Addgene's 20th Anniversary Party

    Type
    Blog Post
    Published
    June 11, 2024, 1:15 p.m.
    ...directors, we can't celebrate Addgene without recognizing our incredible depositors and requestors. Thank...

  13. Call for Guest Bloggers

    Type
    Blog Post
    Published
    March 2, 2016, 3 p.m.
    ...techniques that simplify and expedite research. We recognize, however, that we can’t do it all ourselves. Therefore...

  16. What's New in CRISPR - May 2019

    Type
    Blog Post
    Published
    May 28, 2019, 12:49 p.m.
    ...protein from Neisseria meningitidis, Nme2Cas9, that recognizes a simple dinucleotide PAM (N4CC), lending to ...

  20. Why Do I Need an MTA Anyway?

    Type
    Blog Post
    Published
    May 1, 2014, 8:23 p.m.
    ... The UBMTA used by Addgene is internationally recognized as being protective of both the providing institution...
Showing: 81 - 100 of 225 results